Volume 16, Issue 4 (Winter 2019)                   Sci J Iran Blood Transfus Organ 2019, 16(4): 259-269 | Back to browse issues page

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Sarihi R, Amirizadeh N, Oodi A. Study of Kell blood group genotype in alloimmiunized thalassemia patients. Sci J Iran Blood Transfus Organ 2019; 16 (4) :259-269
URL: http://bloodjournal.ir/article-1-1275-en.html
Study of Kell blood group genotype in alloimmiunized thalassemia patients
R. Sarihi * , N. Amirizadeh , A. Oodi
Keywords: Key words: Genotype, Kell Blood-Group System, Thalassemia
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Sci J Iran Blood Transfus Organ 2019;16 (4): 259-269
Original Article
 

 

Study of Kell blood group genotype in alloimmiunized thalassemia patients
 
Sarihi R.1, Amirizadeh N.1, Oodi A.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
Abstract
Background and Objectives
Alloimmunization is the most serious problem in thalassemia patients and Anti-K is the most prevalent antibody in these patients. So accurate identification of this antigen can significantly decrease the rate of alloimmunization. Serological phenotyping is usually not reliable in multi-transfused patients. Molecular genotyping can overcome limitations of hemagglutination assays, resolve discrepant serologic typing and guide RBC selection for them. In this regard, we intended to determine the K and k among alloimmunized thalassemia patients using molecular methods and compare the results between different methods. 
 
Materials and Methods
In this descriptive study, a total of 200 blood samples were collected randomly from alloimmunized thalassemia patients of Tehran Adult Thalassemia Clinic. The phenotype of all samples was determined for K and k. PCR-SSP was performed for all samples. The discrepant results between the phenotype and genotype were re-evaluated by PCR-RFLP and were confirmed by DNA sequencing.
 
Results
Sixty-three (28%) out of 200 patients developed Anti-K. Molecular typing of samples for K and k antigens revealed 96% (192 patients) KEL*02/KEL*02 and 4% (8 patients) KEL*01/KEL*02 among our samples. Discrepancy between the serology and genotyping results were detected in 8 cases that in all cases correction of genotype results was confirmed by DNA sequencing.
 
Conclusions 
This study demonstrates that antibodies against K antigen continue to develop in thalassemia patients at high rate. Our findings suggest that RBC molecular genotyping is superior to serological phenotyping and is a good alternative and more reliable method especially in multi transfusion patients such as thalassemia patients.
 
Key words: Genotype, Kell Blood-Group System, Thalassemia
 
 
 
 
 
Received: 22 May 2019
Accepted: 18 Aug 2019
 
 

Correspondence: Oodi A., PhD of Hematology and Blood Banking. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601606; Fax: (+9821) 88601606
E-mail: a.oodi@ibto.ir
Type of Study: Research | Subject: Imunohematology
Published: 2019/12/31
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