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Sci J Iran Blood Transfus Organ 2016, 13(3): 176-184 Back to browse issues page
Molecular evaluation of Human Neutrophil Antigen (HNA-3) in some blood donors
F. Shahin, M. Shaiegan , Sh. Samie, B. Bayat, F. Nadali, Z. Ataei, M. Sayyadi, A. Chegini
Keywords: Key words: HNA-3a antigen٬ human, Blood Donors, EDTA
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Type of Study: Research | Subject: Imunohematology
Published: 2016/09/7
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Original  Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2016; 13(3): 176-184
 
 

Molecular evaluation of Human Neutrophil
Antigen (HNA-3) in some blood donors
 
Shahin F.1, Shaiegan M.1, Samiee Sh.1, Bayat B.2, Nadali F.3, Ataie Z.1
Sayyadi M.1, Chegini A.1
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Institute for Clinical Immunology and Transfusion Medicine, Justus Liebig University, Giessen, Germany
3School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran 
 
 
Abstract
Background and Objectives
Neutrophil alloantigens are involved in several blood transfusion reactions including transfusion related acute lung injury (TRALI), refractoriness to granulocyte transfusions and febrile transfusion reactions (FNHTRs). Due to lack of reports on neutrophil antigens,  we aimed to detect the frequency rates of HNA-3a and HNA-3b antigens in some blood donors living in Tehran.
 
Materials and Methods
Blood samples were collected in EDTA (anticoagulant) containing tubes from 110 blood donors having referred to Tehran Blood Center. DNA was extracted by silica filtered columns. PCR-SSP was used for evaluation of HNA-3a, HNA-3b. The gene frequency rates of HNA 3a and HNA-3b antigenes were calculated by  Hardy Weinberg equilibrium.
 
Results
The gene frequencies of HNA-3a, HNA-3b were 0.74 and 0.26, respectively showing consistency with the Hardy-Weinberg equilibrium. HNA-3a phenotypic frequency was found in 103 (93.6%) blood donors while 7 (6.4%) showed lacking it. HNA-3b was found in 50 (45.5%) blood donors while 60 (54.5%) lacking. There was not found any differences in gene and antigene frequency rates between the two genders.
 
Conclusions
The frequencies of HNA-3a and -3b were similar to those found in the previous study conducted on German and Turkish populations.
 
Key words: HNA-3a antigen٬ human, Blood Donors, EDTA
 
 
 
 
 
 
 
Received: 28  Nov 2015
Accepted: 30 Apr 2016
 
 
 

Correspondence: Shaiegan M., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601564; Fax: (+9821) 88601599
E-mail:
M.Shaiegan@ibto.ir
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Shahin F, Shaiegan M, Samie S, Bayat B, Nadali F, Ataei Z, et al . Molecular evaluation of Human Neutrophil Antigen (HNA-3) in some blood donors. Sci J Iran Blood Transfus Organ. 2016; 13 (3) :176-184
URL: http://bloodjournal.ir/article-1-1002-en.html


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Volume 13, Issue 3 (Autumn 2016) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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