[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Editorial Board
Advisory Board
Aims & Scope
Journal News
Articles archive::
All Issues
Current Issue
Articles in press
For Authors::
Submission Instruction
For Reviewers::
Reviewers Section
Subscription::
Subscription Form
News& Events::
Journal News
Contact us::
Contact Information
Contact us
Site Facilities::
Site map
Search contents
Top 10 contents
Inform others
Ethics & Permissions::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing
                        
..
:: Volume 12, Issue 3 (Autumn 2015) ::
Sci J Iran Blood Transfus Organ 2015, 12(3): 244-254 Back to browse issues page
Retinal cells development through transfection of CD133+ HSC derived from human cord blood by PAX6 master gene
S. Balagholi , S. Amini-Kafi Abad , Z.S. Soheili , Sh. Samiee , H. Ahmadiyeh , M. Rezaei Kanavi , M. Nikougoftar Zarif , N. Daftarian
Keywords: Key words: PAX6 protein, CD133 antigen, Cord Blood
Full-Text [PDF 456 kb]   (1867 Downloads)     |   Abstract (HTML)  (7397 Views)
Type of Study: Research | Subject: Hematology
Published: 2015/09/2
Full-Text:   (2684 Views)
    References:
  1. Vugler AA. Progress toward the maintenance and repair of degenerating retinal circuitry. Retina 2010; 30(7): 983-1001.
  2. Osakada F, Jin ZB, Hirami Y, Ikeda H, Danjyo T, Watanabe K, et al. In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induction. J Cell Sci 2009; 122(Pt 17): 3169-79.
  3. Zhao X, Liu J, Ahmad I. Differentiation of embryonic stem cells into retinal neurons. Biochem Biophys Res Commu 2002; 297(2): 177-84.
  4. Cui YX, Kafienah W, Suleiman MS, Ascione R. A new methodological sequence to expand and transdifferentiate human umbilical cord blood derived CD133+ cells into a cardiomyocyte-like phenotype. Stem Cell Rev 2013; 9(3): 350-9.
  5. Paspala SA, Balaji AB, Nyamath P, Ahmed KS, Khan AA, Khaja MN, et al. Neural stem cells & supporting cells - The new therapeutic tools for the treatment of spinal cord injury. Indian J Med Res 2009; 130(4): 379-91.
  6. Faghihi F, Mehranjani MS, Mehrjerdi NZ, Baharvand H. Effect of Staurosporine on Neural Differentiation of CD133+ Umbilical Cord Blood Cells. Yakhteh Medical Journal 2008; 10(1): 33-40.
  7. Murakami Y, Ogasawara M, Sugahara F, Hirano S, Satoh N, Kuratani S.  Identification and   expression  of
the lamprey Pax6 gene: evolutionary origin of the segmented brain of vertebrates. Development 2001; 128(18): 3521-31.
  1. Sansom SN, Griffiths DS, Faedo A, Kleinjan DJ, Ruan Y, Smith J, et al. The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesi PLoS Genet 2009; 5(6): e1000511.
  2. Cvekl A, Yang Y, Chauhan BK, Cveklova K. Regulation of gene expression by Pax6 in ocular cells: a case of tissue-preferred expression of crystallins in lens. Int J Dev Biol 2004; 48(8-9): 829-44.
  3. Azuma N, Tadokoro K, Asaka A, Yamada M, Yamaguchi Y, Handa H, et al. The Pax6 isoform bearing an alternative spliced exon promotes the development of the neural retinal structure. Hum Mol Genet 2005; 14(6): 735-45.
  4. Hsieh YW, Yang XJ. Dynamic Pax6 expression during the neurogenic cell cycle influences proliferation and cell fate choices of retinal progenitors. Neural Dev 2009; 4(32): 1-19.
  5. Koike-Kiriyama N, Adachi Y, Minamino K, Iwasaki M, Nakano K, Koike Y, et al. Human cord blood cells can differentiate into retinal nerve cells. Acta Neurobiol Exp (Wars) 2007; 67(4): 359-65.
  6. Huang W, Fileta J, Guo Y, CL G. Downregulation of Thy1 in retinal ganglion cells in experimental
glaucoma. Curr Eye Res 2006; 31(3): 265-71.
  1. Burmeister M, Novak J, Liang MY, Basu S, Ploder L, Hawes NL, et al. Ocular retardation mouse caused by Chx10 homeobox null allele: impaired retinal progenitor proliferation and bipolar cell differentiation. Nat Genet 1996; 12(4): 376-84.
  2. Qiang L, Fujita R, Yamashita T, Angulo S, Rhinn H, Rhee D, et al. Directed conversion of Alzheimer’s disease patient skin fibroblasts into functional neurons. Cell 2011; 146(3): 359-71.
  3. Han DW, Tapia N, Hermann A, Hemmer K, Höing S, Araúzo-Bravo MJ, et al. Direct reprogramming of fibroblasts into neural stem cells by defined factors. Sell Stem Cell 2012; 10(4): 465-72.
  4. Nishina S, Kohsaka S, Yamaguchi Y, Handa H, Kawakami A, Fujisawa H, et al. PAX6 expression in the developing human eye. Br J Ophthalmol 1999; 83(6): 723-7.
  5. Qiu G, Seiler MJ, Thomas BB, Wu K, Radosevich M, Sadda SR. Revisiting nestin expression in retinal progenitor cells in vitro and after transplantation in vivo. Exp Eye Res 2007; 84(6): 1047-59.
  6. Yang J, Bian W, Gao X, Chen L, Jing N. Nestin expression during mouse eye and lens development. Mech Dev 1999; 94(1-2): 287-91.
  7. Giorgetti A, Marchetto MC, Li M, Yu D, Fazzina R, Mu Y, et al. Cord blood-derived neuronal cells by ectopic expression of Sox2 and c-Myc. Proc Natl Acad Sci U S A 2012; 109(31): 12556-61.
  8. Jang YK, Park JJ, Lee MC, Yoon B, Yang YS, Yang SE, et al. Retinoic acid- mediated induction of neurons and glial cells from human umbilical cord blood- derived hematopoietic stem cells. J Neurosci Res 2003; 75(4): 573-84.
  9. Slovinska L, Novotna I, Kubes M, Radonak J, Jergova S, Cigankova V, et al. Umbilical cord blood cells CD133+/CD133- cultivation in neural proliferation media differentiates towards neural cell lineages. Arch Med Res 2011; 42(7): 555-62.
  10. Zangiacomi V, Balon N, Maddens S, Lapierre V, Tiberghien P, Schlichter R, et al. Cord blood-derived neurons are originated from CD133+/CD34 stem/progenitor cells in a cell-to-cell contact dependent manner. Stem Cells Dev 2008; 17(5): 1005-16.
  11. Azuma N, Tadokoro K, Asaka A, Yamada M, Yamaguchi Y, Handa H, et al. Transdifferentiation of the retinal pigment epithelia to the neural retina by transfer of the Pax6 transcriptional factor. Hum Mol Genet 2005; 14(8): 1059-68.
  12. Cartier L, Laforge T, Feki A, Arnaudeau S, Dubois-Dauphin M, Krause KH. Pax6-induced alteration of cell fate: shape changes, expression of neuronal a tubulin, postmitotic phenotype, and cell migration. J Neurobiol 2006; 66(5): 421-36.
 
 
 
 

 

 
 
Original  Article
 
 
 
 
 
Sci J Iran Blood Transfus Organ 2015; 12(3): 244-254
 
 
 Retinal cells development through transfection
of CD133+ HSC derived from human cord blood by
PAX6 master gene
 
Balagholi S.1,2, Amini Kafi-Abad S.1, Soheili Z.S.3, Samiee Sh.1, Ahmadiyeh H.4,
Rezaie Kanavi M.4, Nikougoftar Zarif M.1, Daftarian N.4
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Tehran Regional Educational Blood Transfusion Center, Tehran, Iran
3National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
4Ophthalmic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
 
Abstract
Background and Objectives
Cord blood CD133+ stem cells are highly proliferative primitive hematopoietic progenitors. These cells can also differentiate to other cells especially neural cells. The PAX6 gene belongs to a family of genes that play a critical role in the formation of tissues and organs during embryonic development. The PAX6 protein is thought to activate genes involved in the formation of the eyes, brain and spinal cord (central nervous system), and the pancreas. The purpose of this study was to assess  PAX6 (5a) gene transfer in cord blood CD133+ stem cells and evaluate its effect on differentiation.
 
Materials and Methods
In this experimental study, cord blood stem cells were collected and mononuclear cells isolated by ficoll; then, CD133+ cells were obtained using the CD133 MicroBead Kit in combination with the autoMACS Separator. These cells were cultured in Stem Span media. Next, HEK293T packaging cells were co-transfected with PLEX-MCS, PsPAX2, and pMD2G by calcium phosphate method. Lentiviral vectors were collected and concentrated. The appropriate amount of viruses was used to infect CD133+ cells. The successful transduced cells were selected by puromycin resistance. After two weeks, the expression of Rhodopsin, CHX10, Thy1, Nestin, and PAX6 proteins was assessed by immunocytochemisrty method.
 
Results
Two weeks after infection, the expression of Rhodopsin, CHX10, Thy1, Nestin, and PAX6 proteins were detected in treatment cells and the expression of Rhodopsin and Nestin in control cells.
 
Conclusions 
The results showed CD133+ cells differentiated into progenitor neural like cells and retinal neural like cells including ganglion like cells.
 
Key words: PAX6 protein, CD133 antigen, Cord Blood
 
Received:  26 Apr 2014
Accepted: 28 Feb 2015
 
 
 

Correspondence: Amini Kafi-Abad S., MD. Pathologist. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601558; Fax: (+9821) 88601542
E-mail: s.amini@ibto.ir
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Balagholi S, Amini-Kafi Abad S, Soheili Z, Samiee S, Ahmadiyeh H, Rezaei Kanavi M, et al . Retinal cells development through transfection of CD133+ HSC derived from human cord blood by PAX6 master gene. Sci J Iran Blood Transfus Organ 2015; 12 (3) :244-254
URL: http://bloodjournal.ir/article-1-861-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 12, Issue 3 (Autumn 2015) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4645