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:: Volume 10, Issue 2 (Summer 2013) ::
Sci J Iran Blood Transfus Organ 2013, 10(2): 173-181 Back to browse issues page
Effect of a protrombin activator isolated from Iranian Echis carinatus venom on hemostasis
M. Babaie , H. Zolgagharian , H. Salmanizadeh , A. Zare Mirakabadi , H. Alizadeh
Keywords: Key words: Blood Coagulation, Prothrombin activator (Echis carinatus venom), Venom
Full-Text [PDF 437 kb]   (2597 Downloads)     |   Abstract (HTML)  (17229 Views)
Type of Study: Research | Subject: Biochemistry
Published: 2013/08/24
Full-Text:   (5907 Views)
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Original Article
 
 
 
 
Sci J Iran Blood Transfus Organ 2013; 10(2):173-181
 
 
 

Effect of a protrombin activator isolated from Iranian
Echis carinatus venom on hemostasis
 
 
Babaie M.1, Zolfagharian H.2, Salmanizadeh H.1, Zare Mirakabadi A. 2, Alizadeh H. 1
 
 
 
1Young Researches and Elites Club, Science and Research Branch of Islamic Azad University, Tehran, Iran
2Department of Venomous Animals and Antivenom Production, Razi Vaccine and Serum Research Institute, Karaj, Iran
 
 
Abstract
Background and Objectives
Echis carinatus venom is a complex mixture of toxins. This venom contains metalloproteinases which convert prothrombin to meizothrombin. The prothrombin activator leads to the formation of small blood clots inside the blood vessels throughout the body. To understand the effect mechanism of Iranian Echis carinatus venom, in this study we investigated the effect of EV on human plasma proteins (prothrombin and fibrinogen) and on blood coagulation. The aim was the purification and characterization of procoagulant factor from the Iranian Echis Carinatus venom and the evaluation of the procoagulant activity on human plasma.
 
Materials and Methods
Crude venom from the Iranian snake species E. carinatus was selected. The prothrombin activator was purified from the crude venom of Echis carinatus by combination of the procedures by gel filtration, ion-exchange chromatography, and reverse phase HPLC. Electrophoresis on 12.5% polyacrylamide gel was performed.
 
Results
The Iranian E. carinatus venom was able to coagulate human plasma very rapidly. The coagulation time was reduced from 13.4 seconds (SD= ±0.59) to 8.6 seconds (SD= ±0.64) when human plasma was treated with crude venom (concentraion of venom was 1 mg/ml).
 
Conclusions 
The venom of Iranian Echis carinatus contains procoagulant factors. It seems the fraction F1B4 isolated from IEc to be like coagulation proteins which coagulate human plasma very rapidly in vitro.
 
Key words: Blood Coagulation, Prothrombin activator (Echis carinatus venom), Venom
 
 
Received: 10 Jan 2012
Accepted:18 Apr 2012
 
 
 

Correspondence: Zolfagharian H., PhD of Clinical Biochemistry. Assistant Professor of Razi Vaccine and Serum Research Institute.         
P.O.Box: 31975-148, Karaj, Iran. Tel: (+98261) 4580038; Fax: (+98261) 34502859
                E-mail: h.zolfagharian@rvsri.ir
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Babaie M, Zolgagharian H, Salmanizadeh H, Zare Mirakabadi A, Alizadeh H. Effect of a protrombin activator isolated from Iranian Echis carinatus venom on hemostasis. Sci J Iran Blood Transfus Organ 2013; 10 (2) :173-181
URL: http://bloodjournal.ir/article-1-773-en.html
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Volume 10, Issue 2 (Summer 2013) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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