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:: Volume 6, Issue 3 (Autumn 2009) ::
Sci J Iran Blood Transfus Organ 2009, 6(3): 191-198 Back to browse issues page
Molecular study of HCV RNA among anti-HCV negative blood donors
M. Mahmoodian Shooshtari Dr. , H. Bahrami, Z. Sharifi Dr.
Abstract:   (35508 Views)

  Abstract

 Background and Objectives

 In order to identify acutely infected blood donors before seroconversion and to reduce the potential risk of transfusion-associated hepatitis C, the detection of HCV RNA by NAT has therefore been introduced recently in several developed countries. In this study, the molecular screening of HCV RNA among anti-HCV negative blood donors was carried out in Iranian Blood Transfusion Organization Research Center, Tehran.

 

 Materials and Methods

 Eight thousand samples negative for anti-HCV (EIA, third generation) were screened for HCV RNA in 25 mini-pools. A total of 320 mini-pools were tested using RT-PCR method for qualitative detection of HCV RNA, with a lower limit of detection of 200 IU/ml.

 

 Results

 All samples tested by RT-PCR method were negative for HCV RNA. On initial testing, two false positive results were defined as positive but on repeat single testing they came out to be negative.

 

 Conclusions

 HCV RNA detection by PCR can be carried out routinely without any significant delay in release of blood components. The residual risk of transmission can be reduced by identification of early infection which can lead to an improvement in safety of blood components. It was also shown that combined screening using anti-HCV and 25-mini-pool HCV RNA testing can be both useful and cost-effective.

 

  Key words : RT-PCR , Blood donors , ELISA , Plasma , Anti-HCV Antibodies

Keywords: RT-PCR, Blood donors, ELISA, Plasma, Anti-HCV Antibodies
Full-Text [PDF 239 kb]   (2660 Downloads)    
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2014/08/17
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Mahmoodian Shooshtari M, Bahrami H, Sharifi Z. Molecular study of HCV RNA among anti-HCV negative blood donors. Sci J Iran Blood Transfus Organ. 2009; 6 (3) :191-198
URL: http://bloodjournal.ir/article-1-347-en.html


Volume 6, Issue 3 (Autumn 2009) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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