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:: Volume 20, Issue 1 (Spring 2023) ::
Sci J Iran Blood Transfus Organ 2023, 20(1): 11-18 Back to browse issues page
Evaluation of the development of specific lymphoblastoid cell line producing antibody from B lymphocytes in thalassemia patients immunized against KELL antigen by Epstein-Barr virus
P. Ahmadi Sead Mohammadi , Z. Sharifi , A. Oodi , S. Milani
Keywords: Alloimmunization, Epstein-Barr Virus, B Lymphocytes
Full-Text [PDF 390 kb]   (352 Downloads)     |   Abstract (HTML)  (416 Views)
Type of Study: Research | Subject: Imunohematology
Published: 2023/04/4
Full-Text:   (338 Views)
References:
  1. Holzlöhner P, Hanack K. Generation of murine monoclonal antibodies by hybridoma technology. J Vis Exp 2017; 2017(119): 54832.
  2. Zimring JC, Hendrickson JE. The role of inflammation in alloimmunization to antigens on transfused red blood cells. Curr Opin Hematol 2008; 15(6): 631-5.
  3. Golestani R, Pourfathollah AA, Moazzeni SM. An extreme strategy for the production of hybridoma. Hybridoma 2009; 28(2): 139-44.
  4. Dorgalaleh A, Gholami MS, Shokuhiyan M, Valikhani M, Moghaddam ES, Naderi M. Alloimmunization against Rh and Kell blood groups antigens is the main obstacle for blood transfusion in transfusion dependent thalassemia patients in Iran. Int J Case Reports Images 2017; 8(5): 358.
  5. Dhawan HK, Kumawat V, Marwaha N, Sharma RR, Sachdev S, Bansal D, et al. Alloimmunization and autoimmunization in transfusion dependent thalassemia major patients: Study on 319 patients. Asian J Transfus Sci 2014; 8(2): 84-8.
  6. Nganou BK, Selvaraj J, Tane P, Nchiozem A, Simo I, Mvnl Ch, Palanisamy D. A Review on Adenocarpus mannii; a Main Species of the Genus Adenocarpus. Current Traditional Medicine 2020; 7(6): 3-12.
  7. El Kababi S, Benajiba M, El Khalfi B, Hachim J, Soukri A. Red blood cell alloimmunizations in beta-thalassemia patients in Casablanca/Morocco: Prevalence and risk factors. Transfus Clin Biol 2019; 26(4): 240-8.
  8. Harada G, Matsumoto SE, Yamashita M, Fujii K, Shirahata S, Katakura Y. In vitro immunization of Epstein-Barr virus-immortalized B cells augments antigen-specific antibody production. Cytotechnology 2013; 65(6): 979-83.
  9. Bishop GA, Busch LK. Molecular mechanisms of B-lymphocyte transformation by Epstein-Barr virus. Microbes Infect 2002; 4(8): 853-7.
  10. Babcock GJ, Decker LL, Volk M, Thorley-Lawson DA. EBV persistence in memory B cells in vivo. Immunity 1998; 9(3): 395-404.
  11. Pelloquin F, Lamelin JP, Lenoir GM. Human B lymphocytes immortalization by Epstein-Barr virus in the presence of cyclosporin A. In Vitro Cell Dev Biol 1986; 22(12): 689-94. 
  12. Aiyer Harini P, Ashok Kumar HG, Praveen Kumar G, Shivakumar N. An overview of immunologic adjuvants-A review. J Vaccines Vaccin 2013; 4(1): 1000167.
  13. Liddicoat AM, Lavelle EC. Modulation of innate immunity by cyclosporine A. Biochem Pharmacol 2019; 163: 472-80. 
  14. Hui-Yuen J, McAllister S, Koganti S, Hill E, Bhaduri-Mcintosh S. Establishment of Epstein-Barr virus growth-transformed lymphoblastoid cell lines. J Vis Exp 2011; (57): 3321.
  15. Sarihi R, Amirizadeh N, Oodi A, Azarkeivan A. Distribution of Red Blood Cell Alloantibodies Among Transfusion-Dependent β-Thalassemia Patients in Different Population of Iran: Effect of Ethnicity. Hemoglobin 2020; 44(1): 31-6.
  16. Foung SKH, Perkins S, Raubitschek A, Larrick J, Lizak G, Fishwild D, et al. Rescue of human monoclonal antibody production from an EBV-transformed B cell line by fusion to a human-mouse hybridoma. J Immunol Methods 1984; 70(1): 83-90.
  17. Pasha  RPK ,  Roohit   A, Shokri F. Establishment of  human heterohybridoma and lymphoblastoid cell lines specific for the Rh D and C antigens. Transfus Med 2003; 13(2): 83-92.
  18. Fraussen J, Vrolix K, Martinez-Martinez P, Losen M, Meulemans E, De Baets MH, et al. A novel method for making human monoclonal antibodies. J Autoimmun 2010; 35(2): 130-4.
  19. Lemskaya NA. A modified protocol for highly efficient EBV-mediated immortalization of human B lymphocytes from small volumes of peripheral blood serum. Egypt J Med Hum Genet 2018; 19(3): 221-3.
  20. Tedesco D, Haragsim L. Cyclosporine: a review. J Transplant 2012; 2012: 230386.
  21. Liu C, Liu H, Zhang L, Deng N. An Optimized method for construction of Epstein-Barr virus-transformed immortalized lymphoblastoid cell lines. J Med Discov 2016; 2(1): 1-4.






 






Sci J Iran Blood Transfus Organ 2023;20(1): 11-18
 
Original Article
 



Evaluation of the development of specific lymphoblastoid cell line producing antibody from B lymphocytes in thalassemia patients immunized against KELL antigen by Epstein-Barr virus

Ahmadi Seyd Mohammadi P.1, Sharifi Z.1, Oodi A.1, Milani S.1

1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


Abstract
Background and Objectives
Alloimmunization is caused by exposure of B lymphocytes to foreign antigens following incompatible blood transfusions and is associated with many hemolytic complications. One of the ways to detect blood group antigens is monoclonal antibodies. The aim of the present study is transforming of alloimmunized cells against KELL antigen in thalassemia patients by EBV-Transformation method and to produce lymphoblastoid cell line producing anti-KELL antibody.

Materials and Methods
In an experimental study, peripheral blood mononuclear cells (PBMCs) of 10 thalassemia patients immunized against KELL antigen were isolated by ficoll hypaque method and exposed to EBV-containing supernatant of B95-8 cell line for 4 hours. After one to three weeks, the production of antibody-producing lymphoblastoid clones and the specificity of the antibody were examined by microscopic observation and microhemagglutination method, respectively.

Results
The results showed that exposure of PBMCs of thalassemia patients immunized against KELL antigen with EBV viral soup caused the formation of lymphoblastoid clones. Examination of the supernatant of culture of produced clones showed that the antibody produced by these cells were able to identify red blood cells containing KELL antigen and agglutinate them by micro hemagglutination test.

Conclusions 
Based on the findings, there is a possibility of PBMC transformation in thalassemia patients immunized against KELL antigen and antibody production.

Key words: Alloimmunization, Epstein-Barr Virus, B Lymphocytes




Received:  22 May 2022
Accepted: 11 Sep  2022



Correspondence: Milani S., PhD of Medical Biotechnology. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
 P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88993778; Fax: (+9821) 88993778
E-mail:
s.milani@tmi.ac.ir
 
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Ahmadi Sead Mohammadi P, Sharifi Z, Oodi A, Milani S. Evaluation of the development of specific lymphoblastoid cell line producing antibody from B lymphocytes in thalassemia patients immunized against KELL antigen by Epstein-Barr virus. Sci J Iran Blood Transfus Organ 2023; 20 (1) :11-18
URL: http://bloodjournal.ir/article-1-1449-en.html
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Volume 20, Issue 1 (Spring 2023) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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