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:: Volume 18, Issue 4 (Winter 2021) ::
Sci J Iran Blood Transfus Organ 2021, 18(4): 255-268 Back to browse issues page
Comparison of the effect of platelet lysate derived from umbilical cord blood and peripheral blood on the expansion and differentiation of MSCs
M. Rahimi Mofrad , F. Yari , M. Nikougoftar Zarif , M. Dadashi , A. Aghaie
Keywords: Key words: Platelets, Umbilical Cord Blood, Mesenchymal Stem Cells
Full-Text [PDF 1005 kb]   (573 Downloads)     |   Abstract (HTML)  (1051 Views)
Type of Study: Research | Subject: Hematology
Published: 2021/12/31
Full-Text:   (937 Views)
    References:
 
  1. McPherson RA, Pincus MR. Henry’s clinical diagnosis and management by laboratory methods. 22nd ed. USA: Saunders; 2011. p. 39-40.
  2. Elder S, Thomason J. Effect of Platelet-Rich Plasma on Chondrogenic Differentiation in  Three-Dimensional Culture. Open Orthop J 2014; 8: 78-84.
  3. Kanematsu D , Shofuda T , Yamamoto A, Ban C, Ueda T, Yamasaki M, et al. Isolation and cellular properties of mesenchymal cells derived from the decidua of human term placenta. Differentiation 2011; 82(2): 77-88.
  4. Battula VL, Treml S, Abele H, Bühring HJ. Prospective isolation and characterization of mesenchymal stem cells from human placenta using a frizzled-9-specific monoclonal antibody. Differentiation 2008; 76(4): 326-36.
  5. Harichandan A, Buhring HJ. Prospective isolation of human MSC. Best Pract Res Clin Haematol 2011; 24(1): 25-36.
  6. Phinney DG, Prockop DJ. Concise Review: Mesenchymal Stem/Multipotent Stromal Cells: The State of Transdifferentiation and Modes of Tissue Repair-Current Views. Stem Cells 2007; 25(11): 2896-902.
  7. Zhang ZY, Teoh SH, Hui J, Fisk N, Choolani M, Chan J. The potential of human fetal mesenchymal stem cell for off-the-shelf bone tissue engineering application. Biomaterials 2012; 33(9): 2656-72.
  8. Rauch C, Feifel E, Amann EM, Spötl HP, Schennach H, Pfaller W, et al. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media. ALTEX 2011; 28(4): 305-16.
  9. Bieback K. Platelet lysate as replacement for fetal bovine serum in mesenchymal stromal cell cultures. Transfus Med Hemother 2013; 40(5): 326-35.
  10. Burnouf T, Su CY, Radosevich M, Goubran HA, El-Ekiaby M. Blood-derived biomaterials: fibrin sealant, platelet gel and platelet fibrin glue. ISBT Science Series 2009; 4: 136-42.
  11. Fazzina R, Iudicone P, Mariotti A, Fioravanti D, Procoli A, Cicchetti E, et al. Culture of human cell lines by a pathogen-inactivated human platelet lysate. Cytotechnology 2016; 68(4): 1185-95.
  12. Schallmoser K, Bartmann C, Rohde E, Reinisch A, Kashofer K, Stadelmeyer E, et al. Human platelet lysate can replace fetal bovine serum for clinical- scale expansion of functional mesenchymal stromal cells. Transfusion 2007; 47(8): 1436-46.
  13. Astori G, Amati E, Bambi F, Bernardi M, Chieregato K, Schäfer R, et al. Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future. Stem Cell Res The 2016; 7(1): 93.
  14. Christou I, Mallis P, Michalopoulos E, Chatzistamatiou T, Mermelekas G, Zoidakis J, et al. Evaluation of Peripheral Blood and Cord Blood Platelet Lysates in Isolation and Expansion of Multipotent Mesenchymal Stromal Cells. Bioengineering (Basel) 2018; 5(1): 19.
  15. Kandoi S, L Pk, Patra B, Vidyasekar P, Sivanesan D, S V, et al. Evaluation of platelet lysate as a substitute for FBS in explant and enzymatic isolation methods of human umbilical cord MSCs. Sci Rep 2018; 8(1): 12439.
  16. Mu Y, Wu X, Hao Z. Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditions. BMC Cell Biol 2018; 19(1): 27.
  17. Dessels C, Potgieter M, Pepper MS. Making the Switch: Alternatives to Fetal Bovine Serum for Adipose-Derived Stromal Cell Expansion. Front Cell Dev Biol 2016; 4: 115.
  18. Canestrari E, Steidinger HR, McSwain B, Charlebois SJ, Dann CT. Human Platelet Lysate Media Supplement Supports Lentiviral Transduction and Expansion of Human T Lymphocytes While Maintaining Memory Phenotype. J Immunol Res 2019; 2019: 3616120.
  19. Muraglia A, Todeschi MR, Papait A, Poggi A, Spanò R, Strada P, et al. Combined platelet and plasma derivatives enhance proliferation of stem/progenitor cells maintaining their differentiation potential. Cytotherapy 2015; 17(12): 1793-806.
  20.  Avanzini MA, Bernardo ME, Cometa AM, Perotti C, Zaffaroni N, et al. Generation of mesenchymal stromal cells in the presence of platelet lysate: a phenotypic and functional comparison of umbilical cord blood- and bone marrow-derived progenitors. Haematologica 2009; 94(12): 1469-60.
  21. Sabapathy V, Sundaram B, Mankuzhy P, Kumar S. Human Wharton’s Jelly Mesenchymal Stem Cells Plasticity Augments Scar-Free Skin Wound Healing with Hair Growth. PLoS One 2014; 9(4): e93726.
  22. Amani M, Amirizadeh N, Soleimani M, Malekan H, Habibi Roudkenar M, et al. Effect of growth factors of platelet gel on proliferation and differentiation of mesenchymal stem cells. Sci J Iran Blood Transfus
    Organ 2009; 6(2): 71-83. [Article in Farsi]
  23. Kinzebach S, Dietz L, Kluter H, Thierse HJ, Bieback K. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells. BMC Cell Biol 2013; 14: 48.
  24. Witzeneder K, Lindenmair A, Gabriel C, Höller K, Theiß D, et al. Human-Derived Alternatives to Fetal Bovine Serum  in Cell Culture. Transfus Med Hemother 2013; 40(6): 417-23.
  25. Doucet C, Ernou I, Zhang Y, Begot L, Holy X. Platelet lysates promotes mesenchymal stem cell expansion: A safety substitute for animal serum in cell-based therapy applications. J Cell Physiol 2005; 205(2): 228-36.
  26. Crespo-Diaz R, Behfar A, Butler GW, Padley DJ, Sarr MG, Bartunek J, et al. Platelet Lysate Consisting of a Natural Repair Proteome Supports Human Mesenchymal Stem Cell Proliferation and Chromosomal Stability. Cell Transplant 2011; 20(6): 797-811.
 
 
 
 
 
 

 
 
 
 
Sci J Iran Blood Transfus Organ 2021;18 (4): 255-268
Original Article
 
 
 
 
 


Comparison of the effect of platelet lysate derived from umbilical cord blood and peripheral blood on the expansion
 and differentiation of MSCs
 
Rahimi Mofrad M.1, Yari F.1, Nikougoftar Zarif M.1, Dadashi M.1, Aghaie A.1
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
Abstract
Background and Objectives
Proliferation and differentiation of mesenchymal stem cells (MSCs) in culture media requires the presence of nutrient supplements such as bovine fetal serum (FBS), which increases the risk of prion/xenotic infections. Platelet lysates as a rich source of growth factors and cytokines can replace FBS in cell culture medium. This study aimed to compare the effect of platelet lysates derived from cord blood and peripheral blood on the proliferation and differentiation of MSCs.
 
Materials and Methods
In this experimental study, human MSCs were isolated from placental tissue and identified by flow-cytometry. 3 Three platelet bags were collected and platelet lysates were prepared by repeated freezing/thawing method. MSCs were cultured in different media and evaluated for their differentiation capacity, cultured in osteogenic and adipogenic differentiation media with Alizarin Red and Oil Red-O staining. TGF-β levels were assessed by ELISA. Statistical analysis was performed using SPSS22 software and One-Way ANOVA statistical test.
 
Results
The proliferation rate of MSCs in media containing Cord/Peripheral Blood Lysate (CBL or PBL) was not significantly different from that of media containing 5% FBS. Also, the rate of proliferation in the medium containing CBL plus cord blood serum was not significantly different from the medium containing 10% FBS. Also, the ability of MSCs’ differentiation into osteocytes and adipocytes in media containing CBL or PBL was well preserved.
 
Conclusions 
Platelet lysates can be a suitable alternative to animal serum in cell culture medium. In addition to the growth and proliferation of MSCs, these factors maintain the ability to differentiate into osteocytes and adipocytes in environments containing these factors.
 
Key words: Platelets, Umbilical Cord Blood, Mesenchymal Stem Cells
 
 
 
 
 
Received:  22 Feb 2021
Accepted: 22 Aug 2021
 
 

Correspondence: Aghaie A., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052183; Fax: (+9821) 88601599
E-mail: aghaie.a@gmail.com
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Rahimi Mofrad M, Yari F, Nikougoftar Zarif M, Dadashi M, Aghaie A. Comparison of the effect of platelet lysate derived from umbilical cord blood and peripheral blood on the expansion and differentiation of MSCs. Sci J Iran Blood Transfus Organ 2021; 18 (4) :255-268
URL: http://bloodjournal.ir/article-1-1398-en.html
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Volume 18, Issue 4 (Winter 2021) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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