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:: Volume 16, Issue 4 (Winter 2019) ::
Sci J Iran Blood Transfus Organ 2019, 16(4): 259-269 Back to browse issues page
Study of Kell blood group genotype in alloimmiunized thalassemia patients
R. Sarihi , N. Amirizadeh, A. Oodi Dr.
Abstract:   (409 Views)
Abstract
Background and Objectives
Alloimmunization is the most serious problem in thalassemia patients and Anti-K is the most prevalent antibody in these patients. So accurate identification of this antigen can significantly decrease the rate of alloimmunization. Serological phenotyping is usually not reliable in multi-transfused patients. Molecular genotyping can overcome limitations of hemagglutination assays, resolve discrepant serologic typing and guide RBC selection for them. In this regard, we intended to determine the K and k among alloimmunized thalassemia patients using molecular methods and compare the results between different methods. 
 
Materials and Methods
In this descriptive study, a total of 200 blood samples were collected randomly from alloimmunized thalassemia patients of Tehran Adult Thalassemia Clinic. The phenotype of all samples was determined for K and k. PCR-SSP was performed for all samples. The discrepant results between the phenotype and genotype were re-evaluated by PCR-RFLP and were confirmed by DNA sequencing.
 
Results
Sixty-three (28%) out of 200 patients developed Anti-K. Molecular typing of samples for K and k antigens revealed 96% (192 patients) KEL*02/KEL*02 and 4% (8 patients) KEL*01/KEL*02 among our samples. Discrepancy between the serology and genotyping results were detected in 8 cases that in all cases correction of genotype results was confirmed by DNA sequencing.
 
Conclusions 
This study demonstrates that antibodies against K antigen continue to develop in thalassemia patients at high rate. Our findings suggest that RBC molecular genotyping is superior to serological phenotyping and is a good alternative and more reliable method especially in multi transfusion patients such as thalassemia patients.
 

Keywords: Key words: Genotype, Kell Blood-Group System, Thalassemia
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Type of Study: Research | Subject: Imunohematology
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Sarihi R, Amirizadeh N, Oodi A. Study of Kell blood group genotype in alloimmiunized thalassemia patients. Sci J Iran Blood Transfus Organ. 2019; 16 (4) :259-269
URL: http://bloodjournal.ir/article-1-1275-en.html


Volume 16, Issue 4 (Winter 2019) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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