|
Evaluation of human platelet gel effect on production process and expression level of VEGF in experimental skin wounds in rat
|
M. Mohammadipour   , N. Amirizadeh  , P. Mortazavi , A.R. Jahandideh |
|
|
| Keywords: Key words: Platelet-Rich Plasma, Rats, VEGF |
|
|
Full-Text [PDF 1148 kb]
(1157 Downloads)
| Abstract (HTML) (3374 Views)
|
Type of Study: Research |
Subject:
Hematology
Published: 2019/07/15
|
|
|
|
|
|
|
Full-Text: (3183 Views) |
References:
- Takzaree N, Hassanzadeh G, Rouini MR, Keshtkar A, Manayi A, Haajiakhondi A. Histological study of wound repair with topical aloe vera gel in rat. Tehran University Medical Journal 2015; 73(9): 660-7. [Article in Farsi]
- Ghaderi R, Afshar M, Akhbarie H, Golalipour MJ. Comparison of the Efficacy of Honey and Animal Oil in Accelerating Healing of Full Thickness Wound of mice skin. Int J Morphol 2010; 28(1): 193-8.
- Ghaderi R. Novel advancements in wound healing. J Birjand Univ Med Sci 2014; 21(1): 1-19. [Article in Farsi]
- Yahyavi Y, Teimuri NH, Amani M, Halabian R, Edalati M, Mohammadipour M, et al. Application of expired platelets in the preparation of platelet gel and study of proliferative effects of expired platelet derived growth factor on variety of cell lines. Sci J Iran Blood Transfus Organ 2010; 7(3): 127-37. [Article in Farsi]
- Seyoum M, Enawgaw B, Melku M. Human blood platelets and viruses: defense mechanism and role in the removal of viral pathogens. Thromb J 2018; 16(1): 16.
- Anitua E, Andia I, Ardanza B, Nurden P, Nurden AT. Autologous platelets as a source of proteins for healing and tissue regeneration. Thromb Haemost 2004; 91(01): 4-15.
- Green DM, Klink B. Platelet gel as an intraoperatively procured platelet-based alternative to fibrin glue. Plast Reconstr Surg 1998; 101(4): 1161-2.
- O'Neill E, Zalewski W, Eaton L, Popovsky M, Pivacek L, Ragno G, et al. Autologous platelet‐rich plasma isolated using the Haemonetics Cell Saver 5 and Haemonetics MCS+ for the preparation of platelet gel. Vox Sang 2001; 81(3): 172-5.
- Tambella AM, Attili AR, Dini F, Palumbo Piccionello A, Vullo C, Serri E, et al. Autologous plateletgel to treat chronic decubital ulcers: a randomized, blind controlled clinical trial in dogs. Vet Surg 2014; 43(6): 726-33.
- Edelblute CM, Donate AL, Hargrave BY, Heller LC. Human platelet gel supernatant inactivates opportunistic wound pathogens on skin. Platelets 2015; 26(1): 13-6.
- Amani M, Amirizadeh N, Soleimani M, Malekan H, Habibi Roudkenar M, Bashtar M. Effect of growth factors of platelet gel on proliferation and differentiation of mesenchymal stem cells. Sci J Iran Blood Transfus Organ 2009; 6(2): 71-83. [Article in Farsi]
- Suvik A, Effendy A. The use of modified Masson’s trichrome staining in collagen evaluation in wound healing study. Mal J Vet Res 2012; 3: 39-47.
- Mohan K H, Pai S, Rao R, Sripathi H, Prabhu S. Techniques of immunofluorescence and their significance. Indian J Dermatol Venereol Leprol 2008; 74: 415-9.
- Tatar C, Aydin H, Karsidag T, Arikan S, Kabukcuoglu F, Dogan O, et al. The effects of platelet-rich plasma on wound healing in rats. Int J Clin Exp Med 2017; 10(5): 7698-706.
- Chuncharunee A, Waikakul S, Wongkajornsilp A, Chongkolwatana V, Chuncharunee L, Sirimontaporn A, et al. Invalid freeze-dried platelet gel promotes wound healing. Saudi Pharm J 2019; 27(1): 33-40.
- Parazzi V, Lavazza C, Boldrin V, Montelatici E, Pallotti F, Marconi M, et al. Extensive characterization of platelet gel releasate from cord blood in regenerative medicine. Cell Transplant 2015; 24(12): 2573-84.
- João De Masi EC, Campos AC, João De Masi FD, Ratti MA, Ike IS, João De Masi RD. The influence of growth factors on skin wound healing in rats. Braz J Otorhinolaryngol 2016; 82(5): 512-21.
- Devereaux J, Nurgali K, Kiatos D, Sakkal S, Apostolopoulos V. Effects of platelet-rich plasma and platelet-poor plasma on human dermal fibroblasts. Maturitas 2018; 117: 34-44.
- Cho EB, Park GS, Park SS, Jang YJ, Kim KH, Kim KJ, et al. Effect of platelet‐rich plasma on proliferation and migration in human dermal fibroblasts. J Cosmet Dermatol 2018 Oct 2. doi: 10.1111/jocd.12780.
- Abdullah BJ, Atasoy N, Omer AK. Evaluate the effects of platelet rich plasma (PRP) and zinc oxide ointment on skin wound healing. Ann Med Surg (Lond) 2018; 37: 30-7.
|
Sci J Iran Blood Transfus Organ 2019;16(2): 103-115
|
Evaluation of human platelet gel effect on production
process and expression level of VEGF in experimental
skin wounds in rat
Mohammadipour M.1, Amirizadeh N.2, Mortazavi P.1, Jahandideh A.R.1
1Science and Research Branch, Islamic Azad University, Tehran, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Background and Objectives
Platelet gel prepared from platelet-rich plasma, containing growth factors, accelerates reconstruction of skin layers, stimulation of angiogenesis and increase of collagen synthesis in wounds healing process. In this study, the effect of human platelet gel on was investigated VEGF expression in experimental skin wounds in rat.
Materials and Methods
In this experimental study, platelet gel was prepared by adding thrombin to platelet-rich plasma. 1.5×1.5 cm wound was created on back of the body in 24 male Wistar rats. Then, the rats were divided into two control and treated groups with the application of platelet gel on the wound. Then, each group was divided into 4 subgroups with three rats for sampling on the 3rd, 5th, 7th, and 14 th days. Skin wound samples were studied by using H & E staining and Mason Trichrome methods for histopathological examination. Furthermore, fluorescent immunohistochemistry method was used to measure the expression level of VEGF.
Results
The rate of epithelial tissue formation in the fifth day was 2 ± 0.3 and 1 ± 0.1 in the treated and control groups, respectively. The results of this study revealed that angiogenesis, epithelial tissue formation and collagen formation and inflammatory cells infiltration, in the treated group were higher than the control group, especially at the early stages of wound healing. Moreover, IHC findings showed that VEGF expression level is higher in the treated group than the control.
Conclusions
The topical application of platelet gel on skin lesions has a positive effect on the healing process, especially in the early stages.
Key words: Platelet-Rich Plasma, Rats, VEGF
Received: 24 Feb 2019
Accepted: 29 Apr 2019
Correspondence: Amirizadeh N., PhD of Hematology and Blood Banking. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine .
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601599; Fax: (+9821) 88601599
E-mail: n.amirizadeh@ibto.ir
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
|
Mohammadipour M, Amirizadeh N, Mortazavi P, Jahandideh A. Evaluation of human platelet gel effect on production process and expression level of VEGF in experimental skin wounds in rat. Sci J Iran Blood Transfus Organ 2019; 16 (2) :103-115 URL: http://bloodjournal.ir/article-1-1251-en.html
|
.jpg)
.png)
