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:: Volume 16, Issue 3 (Autumn 2019) ::
Sci J Iran Blood Transfus Organ 2019, 16(3): 161-171 Back to browse issues page
Red blood cells storage lesion: the effect of blood donation time on biochemical parameters
ُS. Jahanshahi Ghajar, M.R. Deyhim Dr., F. Sotoudehnejad Nematollahi Dr., M.H. Rafiee Dr.
Abstract:   (420 Views)
Abstract
Background and Objectives
There is a circadian rhythm and diurnal variation in the redox capacity in blood plasma and there is a decrease in antioxidant capacity (TAC) during the RBCs storage in blood banking. So it is possible that donated blood, based on donation time, has a different antioxidant capacity and as a result a different biochemical response during storage. This study attempts to uncover this issue.
 
Materials and Methods
In this experimental study, Twenty RBC units were collected from two donor groups in the morning (8-11am) and evening (5-8pm) and were stored for 3 to 42 days at 4 to 8 degrees Celsius. Glucose, Sodium, potassium, lactate, lactate dehydrogenase (LDH), pH, malondialdehyde (MDA), and total antioxidant capacity were measured by a commercial kit. Nitrate/nitrite was measured by Griess method.
 
Results
The levels of nitrate/nitrite, MDA, and LDH in the evening group increased significantly when compared to the morning group (p < 0.05). However, a decreasing trend in TAC, pH, Glucose, and sodium levels (p < 0.05) versus an increasing trend in potassium, lactate, LDH, and MDA was seen in both groups during storage (p < 0.05).
 
Conclusions 
This study showed that oxidative damage in donated blood in the evening was higher than in the morning during storage, which may reduce RBC survival. Therefore, more studies are required with more sample sizes to examine the effect of donation time on the RBC storage lesion.
 
 
Keywords: Key words: Blood Donation, Blood Banking, Circadian Rhythm
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Type of Study: Research | Subject: Biochemistry
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Jahanshahi Ghajar ُ, Deyhim M, Sotoudehnejad Nematollahi F, Rafiee M. Red blood cells storage lesion: the effect of blood donation time on biochemical parameters. Sci J Iran Blood Transfus Organ. 2019; 16 (3) :161-171
URL: http://bloodjournal.ir/article-1-1243-en.html


Volume 16, Issue 3 (Autumn 2019) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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