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:: Volume 13, Issue 4 (Winter 2016) ::
Sci J Iran Blood Transfus Organ 2016, 13(4): 249-258 Back to browse issues page
Determination of Hepatitis C virus subtypes in thalassemia patients by phylogenetic analysis by NS5B region
A. Azadi , A. Azarkeivan Dr., F. Zamani Dr., M.S. Eslami Dr., M.R. Eshrahian Dr., K. Samimi-Rad Dr.
Abstract:   (1056 Views)

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Abstract

Background and Objectives

Patients with thalassemia are one of the at risk groups for acquiring Hepatitis C virus (HCV) infection. They need to be transfused regularly. High prevalence of this virus in thalassemia population, complications of HCV infection and the important effect of HCV genotype determination in treatment of the virus were the reasons for performing this research. The aims of the present study were to detect thalassemia patients with chronic infection  and determinate the HCV subtypes distribution in this group.

Materials and Methods

The study was concluded on 120 specimens for HCV antibody from thalassemia patients in Tehran and Amol cites. After sequencing of PCR products, determination of HCV subtypes was performed by construction of phylogenic trees. c2, t, Fisher exact test and SPSS 22 have been used for data analysis.

Results

The results showed that 67 (55/8 %) of the 120 specimens were positive for  HCV RNA,  34 (28.3%) from Tehran and 33 (27.5%) from Amol. 65 specimens could be genotyped. Subtype 1a accounted for 53/8 % (n=35), 3a for 30.7 % (n = 20), 1b for 13/8 % (n = 9) and 4a for 1/7 % (n = 1) of infected cases.

Conclusions 

Implementation of effective strategies for detection of thalassemia patients, with HCV chronic infection in thalassemia centers is necessary. The predominance of genotype 1 among thalassemia patients is important in treatment of these patients because genotype 1 indicates lower rate of sustained viral response to current therapy (Interferon and Ribavirin ) compare to HCV genotype 2 and 3.

Keywords: Key words: Hepatitis C virus, Genotype, Thalassemia
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Type of Study: Research | Subject: Virology
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Azadi A, Azarkeivan A, Zamani F, Eslami M, Eshrahian M, Samimi-Rad K. Determination of Hepatitis C virus subtypes in thalassemia patients by phylogenetic analysis by NS5B region. Sci J Iran Blood Transfus Organ. 2016; 13 (4) :249-258
URL: http://bloodjournal.ir/article-1-1042-en.html
Volume 13, Issue 4 (Winter 2016) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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