Effect of epinephrine on the CXCR4 expression of human cord blood derived CD34+ hematopoietic stem cells
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F. Saba , E. Roshandel , M. Soleimani , A. Atashi , A. Gharehbaghian , S. Abroun , S. Kaviani |
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Abstract: (7984 Views) |
Abstract Background and Objectives Recently, circulating hematopoietic stem cells (HSC) have been used for the treatment of most types of leukemia. However, the G-CSF mechanism has not been known yet but it is believed that G-CSF is mostly effective by its indirect functions. The suppression of the nervous system affects G-CSF induced mobilization of HSC. The main gene involved in mobilization is CXCR4 that ligand to SDF-1. So, this study investigates the effect of the main catecholamine of nervous system-Epinephrin- on CXCR4 expression. Materials and Methods In this experimental study, isolated cord blood CD34+ cell of 20 healthy newborn with MACs columns treated with 10 μM Epinephrine and 1 μM Proporanolol. Expression of CXCR4 has been investigated at 1, 3 and 5 hours with qRT-PCR. Receptors of beta adrenergic expression have been studied with RT-PCR. Results Beta adrenergic receptors are expressed on CD34+ cells. Epinephrine led to significantly increased CXCR4 in hours 1 (3.2 ± 0.5), 3 (2.4 ± 0.4) and 5 (2.2 ± 0.4 ). Cells treated with propranolol, return increased CXCR4 induced by epinephrine. Conclusions It can be concluded that G-CSF affects the expression of CXCR4 through increasing secretion of epinephrine in bone marrow with on beta adrenergic receptors. Cells with increased CXCR4 mobilized to release SDF-1 in peripheral blood. |
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Keywords: Key words : Hematopoietic Stem Cells, Epinephrine, Granulocyte Colony-Stimulating Factor |
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Type of Study: Research |
Subject:
Stem cells Published: 2014/12/31
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