:: Volume 9, Issue 3 And بخش 2 (Stem Cell Supplement , Autumn 2012) ::
Sci J Iran Blood Transfus Organ 2012, 9(3 And بخش 2): 226-238 Back to browse issues page
Effect of human hematopoietic stem cells on differentiation of mesenchymal stem cells to osteoblast cells
R. Fadaei, N. Amirizadeh , M. Nikougoftar, M. Habibi Roudkenar
Abstract:   (18087 Views)


 Background and Objectives

 The correlation between hematopoietic stem cells (HSCs) and the cells comprising the niche especially osteoblast cells is critical for maintaining stem cell activities. Yet little evidence supports the concept that HSCs regulate development of the niche. If true, it may explain why many hematopoietic defects are accompanied by changes in the osseous architecture and provide new therapeutic targets for regulating bone formation.


 Materials and Methods

 In this exprimental study, we cocultured bone marrow mesenchymal stem cells (MSCs) with umbilical cord blood HSCs in stem span media for 3 days. Then MSCs were differentiated to osteoblast cells by using osteogenesis kit. Evaluation of osteogenic differentiation of MSCs in different days came out to be: expression of osteopontin and osteocalsin (RT-PCR on days 4 and 6), expression of CD90 (flow cytometry on day 6), expression of alkaline phosphatase enzyme (alkaline phosphatase staining) and mineralization of MSCs (alizarin red staining on day 10).



 Our results showed early expression of osteopontin and osteocalsin in HSCs coculture with MSCs. These findings support the role of HSCs in induction of osteoblastic differentiation of MSCs. Decrease in the expression of CD90, higher activation in cell alkaline phosphatase enzyme, and mineralization confirmed the results.



 In the field of human stem cells, our ex vivo findings demonstrated that HSCs can enhance differentiation of MSCs toward osteoblast cells thereby participating in formation of their niche.



Keywords: Key words : Hematopoietic Stem Cells, Mesenchymal Stem Cell, Coculture, Osteogenesis, Osteoblasts
Full-Text [PDF 560 kb]   (2101 Downloads)    
Type of Study: Research | Subject: Stem cells
Published: 2013/08/27

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Volume 9, Issue 3 And بخش 2 (Stem Cell Supplement , Autumn 2012) Back to browse issues page