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Evaluation of allelic frequency of neutrophil antigen (HNA-1) in thalassemia patients with recurrent blood transfusion and FNHTR complication
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B. Rahmati   , M. Zadsar  , Sh. Samiee , M. Shaigan |
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| Keywords: FNHTR, Neutrophils, Blood Transfusion, Thalassemia |
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Type of Study: Research |
Subject:
Imunohematology
Published: 2022/10/2
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Sci J Iran Blood Transfus Organ 2022;19 (3): 216-223
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Evaluation of allelic frequency of neutrophil antigen (HNA-1)
in thalassemia patients with recurrent blood transfusion
and FNHTR complication
Rahmati B.1, Zadsar M.1, Samiee Sh.1, Shaigan M.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Background and Objectives
HNAs are glycoprotein antigens on the surface of neutrophils. Antibodies against these antigens are produced in patients with repeated blood transfusions. We here study the
HNA-1a, HNA-1b and HNA-1c gene frequencies in regulary transfused patients suffering from hemolytic transfusion reactions as well as the risk of alloimmunization of these individuals based on the genotype pattern.
Materials and Methods
As the study population, 99 thalassemia patients were included in the descriptive study. Sampling lasted from July 2019 to March 2020 at Zafar Thalassemia Center. Patients were administered low-leukocyte blood. Three mL of blood was collected in tubes containing EDTA and DNA was extracted. HNA-1 gene was analyzed by PCR-SSP method. Allelic frequency of neutrophil antigens was calculated using Hardy-Weinberg equation. Chi-square and T-test were used to analyze the data.
Results
The frequency rates of observed alleles were 52.7% for HNA-1a, 44.3% for HNA-1b, 3% for HNA-1c, which was in accordance with Hardy-Weinberg's law with a 5% confidence interval. The highest risk of alloimmunization to HNA-1b, HNA-1a, and HNA-1c were 22%, 18%, and 3%, respectively.
Conclusions
The frequency of HNA-1 neutrophil antigen allele in thalassemia patients with febrile blood transfusion was consistent with previous findings in different Iranian populations. Patients still have a risk of alloimmunization and blood transfusion based on neutrophilic genotype pattern.
Key words: FNHTR, Neutrophils, Blood Transfsuion, Thalassemia
Received: 28 Feb 2022
Accepted: 30 May 2022
Correspondence: Zadsar M., MD. Specialist in Infectious Diseases. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052234; Fax: (+9821) 88628741
E-mail:
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1- Biological safety cabinet
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1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
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1- Biological safety cabinet
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1- Platelet Concentrate
2- Food and Drug Administration
3- Normal Skin Flora
4- Platelet Rich Plasma-Platelet Concentrate
5- Eosin-Methylene blue
6- Thioglycolate
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maryam.zad@gmail.com
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Rahmati B, Zadsar M, Samiee S, Shaigan M. Evaluation of allelic frequency of neutrophil antigen (HNA-1) in thalassemia patients with recurrent blood transfusion and FNHTR complication. Sci J Iran Blood Transfus Organ 2022; 19 (3) :216-223 URL: http://bloodjournal.ir/article-1-1427-en.html
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