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:: Volume 14, Issue 2 (Summer 2017) ::
Sci J Iran Blood Transfus Organ 2017, 14(2): 92-100 Back to browse issues page
The detection of Scavenger Receptor-B1 expression and its role on the function of platelets in patients with atherosclerotic disease
Mahnaz Abasalizadeh , Prisa Nehzati , Mehdi Allahbakhshian , Mohsen Hamidpour , Ali Akbar Khadem Mabudi , Mohamad Reza Tabatabaie
Keywords: Key words: Platelets, Atherosclerosis, Patients
Full-Text [PDF 1963 kb]   (2270 Downloads)     |   Abstract (HTML)  (4951 Views)
Type of Study: Research | Subject: Hematology
Published: 2017/06/18
Full-Text:   (1915 Views)
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Sci J Iran Blood Transfus Organ 2017; 14(2): 92-100
Original Article
 

 

The detection of Scavenger Receptor-B1 expression
and its role on the function of platelets in patients
with atherosclerotic disease
 
Abasalizade M.1, Nehzati P.1, Allahbakhshian M.1, Hamidpour M.1,
Khadem Mabodi A.A.1, Tabatabaie M.R.2
 
 
1School of Allied Medical Sciences, Shahid  Beheshti University of Medical Sciences, Tehran, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
 
 
 
Abstract
Background and Objectives
SR-B1 acts as the main receptor for HDL and plays an important role in reverse cholesterol transport. In contrast to LDL level which causes Atherosclerotic plaque in artery, plasma concentration of HDL correlates inversely with the incidence of coronary artery disease and atherosclerosis. This study investigates the expression of Scavenger Receptor-B1 and its role on the function of platelets in patients with atherosclerotic disease.
 
Materials and Methods
The present case-control study evaluated the SR-B1 receptor of 24 patients with atherosclerotic plaque and 24 healthy volunteers as the normal control. After separation of platelet, the expression of SR-BI was detected using western blotting. Platelet function in response to agonists was examined by aggregometry. Finally, the data were statistically analyzed using SPSS version 16 software and the student T-test.
 
Results
The western blotting assay showed a significant reduction of SR-BI protein expression in AD patients (0.4 AU) compared with the healthy control (1.4 AU), (p < 0.001). The platelet aggregation in response to ADP showed a significant increase in patients (85%) compared to the control (51%), (p < 0.001).
 
Conclusions 
The levels of SR-B1expression correlate inversely with platelet aggregation.
 
Key words: Platelets, Atherosclerosis, Patients
 
 
 
 
 
 
Received: 25 Oct 2016
Accepted:  1 Feb 2017
 
 

Correspondence: Hamidpour M., PhD of Hematology & Blood Banking. Associate Professor of Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences.
Postal code: 1971653383, Tehran, Iran. Tel: (+9821) 22717504; Fax: (+9821) 22717504
E-mail:
mohsenhp@sbmu.ac.ir
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Abasalizadeh M, Nehzati P, Allahbakhshian M, Hamidpour M, Khadem Mabudi A A, Tabatabaie M R. The detection of Scavenger Receptor-B1 expression and its role on the function of platelets in patients with atherosclerotic disease . Sci J Iran Blood Transfus Organ 2017; 14 (2) :92-100
URL: http://bloodjournal.ir/article-1-1083-en.html
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Volume 14, Issue 2 (Summer 2017) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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