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:: Volume 14, Issue 3 (Atumn 2017) ::
Sci J Iran Blood Transfus Organ 2017, 14(3): 164-174 Back to browse issues page
Bacterial contamination Rate of Platelet Concentrates in Tehran Blood Center
M. Dehghanian , H. Avani , B. Mirzaei , F. Shahcheraghi , M. Zadsar
Keywords: Key words: Platelets, Bacteria, Blood Transfusion
Full-Text [PDF 382 kb]   (3910 Downloads)     |   Abstract (HTML)  (4920 Views)
Type of Study: Research | Subject: Blood transfusion medicine
Published: 2017/09/11
Full-Text:   (2155 Views)
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Sci J Iran Blood Transfus Organ 2017; 14(3): 164-174
Original Article
 

 

Bacterial contamination Rate of Platelet Concentrates
in Tehran Blood Center
 
Dehghanian M.1, Avani H.1, Mirzaei B.2, Shahcheraghi F.2, Zadsar M.1
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
 
Abstract
Background and Objectives
Bacterial contamination of platelet concentrates (PCs) is a longstanding problem in transfusion medicine. The present study aimed to evaluate the frequency of bacterial contamination in the platelet concentrates of Tehran Blood Center by culture-based method.
 
Materials and Methods
In this cross sectional study, totally 1500 platelet concentrates were randomly selected and assayed by standard aerobic and anaerobic bacterial culture methods. The PCs were collected at different time points during storage. One milliliter of each platelet concentrate was inoculated in 10 milliliter fluid Thioglycollate. The presence of bacteria was subsequently monitored. Any positive culture was further evaluated by subculture in the blood agar in both aerobic and anaerobic conditions.
 
Results
Of the 1500 PCs analyzed, one was found contaminated by gram negative bacteria. By further investigation it was confirmed that the responsible organism pertained to the pseudomonas spp. It was isolated on day 4 of incubation. No evidence of infection in donor was found by reviewing the donor questionnaire.
 
Conclusions 
Based on the findings of the current study, our result the contamination rate of PCs has reduced dramatically; however, the isolationfinding of the Pseudomonas spp. is suggestive of environmental contamination. The previous studies have shown that improved donor screening, better skin disinfection, and removal of initial aliquot of donors' blood play an important role in controlling the bacterial contamination rate. OurThese findings suggest that more attention should be paid to optimizing new methods for disinfection techniques and sterilization procedures for preparation premises and storage equipment.
 
Key words: Platelets, Bacteria, Blood Transfusion
 
 
 
 
 
 
Received:  21 Jul 2017
Accepted: 29 Apr 2017
 
 

Correspondence: Zadsar M., MD. Specialist in Infectious Diseases and Tropical Medicine. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 8898997; Fax: (+9821) 88963034
E-mail: maryam_zad@yahoo.com
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Dehghanian M, Avani H, Mirzaei B, Shahcheraghi F, Zadsar M. Bacterial contamination Rate of Platelet Concentrates in Tehran Blood Center. Sci J Iran Blood Transfus Organ 2017; 14 (3) :164-174
URL: http://bloodjournal.ir/article-1-1058-en.html
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Volume 14, Issue 3 (Atumn 2017) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
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