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:: Volume 13, Issue 4 (Winter 2016) ::
Sci J Iran Blood Transfus Organ 2016, 13(4): 249-258 Back to browse issues page
Determination of Hepatitis C virus subtypes in thalassemia patients by phylogenetic analysis by NS5B region
A. Azadi , A. Azarkeivan , F. Zamani , M.S. Eslami , M.R. Eshrahian , K. Samimi-Rad
Keywords: Key words: Hepatitis C virus, Genotype, Thalassemia
Full-Text [PDF 338 kb]   (1479 Downloads)     |   Abstract (HTML)  (5152 Views)
Type of Study: Research | Subject: Virology
Published: 2016/12/24
Full-Text:   (2162 Views)
References:
 
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Sci J Iran Blood Transfus Organ 2017; 13(4): 249-258
Original Article
 

 

Determination of Hepatitis C virus subtypes
in thalassemia patients by phylogenetic analysis
by NS5B region
 
Azadi A.1, Azarkeivan A.2,3, Zamani F.4, Eslami M.S.2,3, Eshragiyan M.1, Samimi-Rad K.1
 
 
1School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2Blood Transfusion Research center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3Adult Thalassemia Clinic, Tehran, Iran
4Firoozgar General Hospital, Tehran, Iran
  
 
Abstract
Background and Objectives
Patients with thalassemia are one of the at risk groups for acquiring Hepatitis C virus (HCV) infection. They need to be transfused regularly. High prevalence of this virus in thalassemia population, complications of HCV infection and the important effect of HCV genotype determination in treatment of the virus were the reasons for performing this research. The aims of the present study were to detect thalassemia patients with chronic infection  and determinate the HCV subtypes distribution in this group.
 
Materials and Methods
The study was concluded on 120 specimens for HCV antibody from thalassemia patients in Tehran and Amol cites. After sequencing of PCR products, determination of HCV subtypes was performed by construction of phylogenic trees. c2, t, Fisher exact test and SPSS 22 have been used for data analysis.
 
Results
The results showed that 67 (55/8 %) of the 120 specimens were positive for  HCV RNA,  34 (28.3%) from Tehran and 33 (27.5%) from Amol. 65 specimens could be genotyped. Subtype 1a accounted for 53/8 % (n=35), 3a for 30.7 % (n = 20), 1b for 13/8 % (n = 9) and 4a for 1/7 % (n = 1) of infected cases.
 
Conclusions 
Implementation of effective strategies for detection of thalassemia patients, with HCV chronic infection in thalassemia centers is necessary. The predominance of genotype 1 among thalassemia patients is important in treatment of these patients because genotype 1 indicates lower rate of sustained viral response to current therapy (Interferon and Ribavirin ) compare to HCV genotype 2 and 3.
 
Key words: Hepatitis C virus, Genotype, Thalassemia
 
Received: 11 May 2016
Accepted:  3 Oct  2016
 
 

Correspondence: Samimi-Rad K., PhD in Medical Microbiology. School of Public Health, Tehran University of Medical Science
P.O.Box: 6446, Tehran, Iran. Tel: (+9821) 88950595; Fax: (+9821) 88989120
E-mail: Ksamimirad@sina.tums.ac.ir
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Azadi A, Azarkeivan A, Zamani F, Eslami M, Eshrahian M, Samimi-Rad K. Determination of Hepatitis C virus subtypes in thalassemia patients by phylogenetic analysis by NS5B region. Sci J Iran Blood Transfus Organ 2016; 13 (4) :249-258
URL: http://bloodjournal.ir/article-1-1042-en.html
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Volume 13, Issue 4 (Winter 2016) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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