2024-03-28T17:09:56+03:30 http://bloodjournal.ir/browse.php?mag_id=38&slc_lang=fa&sid=1
38-659 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 Expansion of CD34+ umbilical cord blood in 3 dimentional culture media on mesenchymal stem cell coated scaffold R. Ranjbaran H. Abolghasemi N. Nasiri A. Oodi M. Nikougoftar N. Amirizadeh n.amirizadeh@ibto.ir   Abstract  Background and Objectives Stem cell transplantation has achieved high success for treatment but the use of this source has some limitation. Cord blood transplantation is clinically limited by its low progenitor cell contents such as CD34+. The aim of this study was to evaluate the effect of mesenchymal stem cells (MSCs) on replication of CD34+ in cord blood.   Materials and Methods In this experimental study MSCs were isolated and cultured from BM mononuclear cells. MSCs were used for co-culture in two systems of 2D and 3D cultures. After isolation, umbilical cord blood CD34+ cells were cultured in two different systems. The level of expansion in these systems was evaluated by cell count, analysis of CD34+ expression by flowcytometery, and CFU assay .   Results Expansion of cord blood HSCs was evaluated at three different culture systems at three different times (day3, day 7 and day 10): the medium with cytokine, 2D medium with cytokine + MSCs, and 3D medium with cytokine + MSCs on scaffold. The fold increase of TNC, CFU, and CD34+ cells at day 10 for the former medium was 87 ± 6, 84 ± 8, 11 ± 3, for the second 103 ± 8, 111 ± 9, 14 ± 3, and for the latter 127 ± 10, 170 ± 14, 20 ± 4, respectively. The highest expansion was observed in the 3D co-culture with MSCs after 10 days.    Conclusions The results have demonstrated that the co-culture of CB HSCs with mesenchymal cells could increase the rate of expansion especially in the 3D system.   Key words : Mesenchymal Stem Cells Umbilical Cord Blood CD34 Antigen Stem Cells 2012 8 01 177 185 http://bloodjournal.ir/article-1-659-en.pdf
38-660 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 Effect of miR-146a and miR-150 on T-cell lymphoid differentiation of hematopoietic stem cells soleimani.masoud@gmail.com   Abstract  Background and Objectives Micro RNAs are a class of small non-coding RNAs which have been recently shown to play a crucial role in major cellular processes such as development and differentiation through post-transcriptional regulation. The role of these epigenetic elements has been also demonstrated in hematopoietic lineage differentiation and there is a large body of evidence that miR-150 and miR-146a are responsible for T lymphocyte differentiation. Our goal was to examine the effect of miR-150 and miR-146a overexpression in hematopoietic stem cells and its ability to differentiate these cells into a T lymphoid cell.    Materials and Methods In this experimental study CD133+ stem cells were employed as hematopoietic stem cells and permanent overexpression of miR-150 and miR-146a was established. The differentiation progress was tracked by flowcytometry for lymphocyte markers such as CD4 and CD8. Moreover, expression of miR-146a and miR-150 by RT-PCR and quantitative real time PCR after 7 days was studied.    Results The cells showed T lymphoid characteristics 7 days after transduction. CD4 and especially CD8 expression significantly increased.   Conclusions In conclusion, miR-150 and miR-146a overexpression is an effective factor in T cytotoxic differentiation and they have the ability of directing CD133+ hematopoietic stem cells to express T lymphoid characteristics.     Key words : MicroRNAs miR-150 human miR-146a human Hematopoietic Stem Cells 2012 8 01 186 198 http://bloodjournal.ir/article-1-660-en.pdf
38-661 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 Effect of mineralized bone allograft scaffold with coverage of UCB-derived stromal cells on ex vivo expansion of CD34+ cells Z.S. Hashemi M. Forouzandeh Moghadam foroz@modares.ac.ir M. Soleimani Gh. Khamisipour M. Mossahebi Mohammadi     Abstract  Background and Objectives Bone marrow transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming by minimum manipulation, mineralized bone allograft (MBA) scaffold coated by UCB-USSC (unrestricted somatic stem cells) was used to expand CD34+ cells from UCB.    Materials and Methods In this experimental study, UCB-USSCs were isolated and characterized by morphologic and immunophenotypical analysis. Then they were seeded on MBA (for 3D figuration) and culture plate (for 2D figuration) as a feeder layer. CD34+ cells were isolated from the UCB (by MACS method) and were expanded in 2D and 3D conditions for 3 weeks. At the end, cell count, flow cytometry, cologenic assay, and LTC-IC were done.   Results After 3 weeks, ex vivo expansion of UCB-CD34+ was enhanced 250 ± 13.2 in 3D. The highest CFC expansion and LTC-IC were observed at day 14. Flow cytometry analysis showed the lowest percentage in 3D culture.   Conclusions USSCs by 3D MBA structure produce the hematopoietic cytokine, extracellular matrix and binding molecules they also create the intercellular interaction that can be used as a suitable feeder layer. It provides an ex vivo mimicry of bone marrow niche by enhancing surface/volume ratio. This model could be a suitable and alternative environment for HSCs expansion and hematopoiesis.    Key words : MBA Hematopoietic Stem Cells Umblical Cord Blood Allograft 2012 8 01 199 213 http://bloodjournal.ir/article-1-661-en.pdf
38-662 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 Cloning and transient expression of cytoprotective factor, HO-1, in mesenchymal stem cells using the adenoviral expression system through Gateway technology P. Hamedi Asl R. Halabian M. Mohammadzadeh M. Mohammadipour Z. Bakhshandeh Dr. Hamedi Asl A.A. kianin M.A. Jalili N. Amirizadeh M. Habibi Roudkenar roudkenar@ibto.ir   Abstract  Background and Objectives Heme oxegenase1 (HO-1) is one of the potent cytoprotective factors. The goal of this study was to perform cloning and transient over expression of the human HO-1 gene in mesenchymal stem cells (MSCs) using the adenoviral expression system based on the gateway technology.   Materials and Methods In order to induce expression of HO-1, A549 cell lines were exposed to UV for 1 hour. The full length cDNA of HO-1 was isolated and cloned into pENTR TOPO/D vector by TOPO cloning reaction. To construct the expression clone, a LR recombination reaction was carried out between the entry clone and destination vector, pAd/CMV/V5-DEST. The recombinant virus was produced in the appropriate mammalian cell line. MSCs were infected by the recombinant virus expressing HO-1.   Results The results showed that human recombinant HO-1 was successfully cloned and the accuracy of the gene and its frame in the vector were confirmed by DNA sequencing. Expression of HO-1 in MSCs was confirmed by RT-PCR and western blot analysis. The results indicated that the expression of HO-1 is transient.   Conclusions  Transient expression of human HO-1 gene in MSCs by using adenovirus expression system may be considered as an efficient gene transfer strategy into MSCs in order to promote stem cell therapy.      Key words: Mesenchymal Stem Cells Heme Oxygenase-1 Genetic Engineering Adenoviruses 2012 8 01 214 225 http://bloodjournal.ir/article-1-662-en.pdf
38-663 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 Effect of human hematopoietic stem cells on differentiation of mesenchymal stem cells to osteoblast cells R. Fadaei N. Amirizadeh n.amirizadeh@ibto.ir M. Nikougoftar M. Habibi Roudkenar   Abstract  Background and Objectives The correlation between hematopoietic stem cells (HSCs) and the cells comprising the niche especially osteoblast cells is critical for maintaining stem cell activities. Yet little evidence supports the concept that HSCs regulate development of the niche. If true, it may explain why many hematopoietic defects are accompanied by changes in the osseous architecture and provide new therapeutic targets for regulating bone formation.   Materials and Methods In this exprimental study, we cocultured bone marrow mesenchymal stem cells (MSCs) with umbilical cord blood HSCs in stem span media for 3 days. Then MSCs were differentiated to osteoblast cells by using osteogenesis kit. Evaluation of osteogenic differentiation of MSCs in different days came out to be: expression of osteopontin and osteocalsin (RT-PCR on days 4 and 6), expression of CD90 (flow cytometry on day 6), expression of alkaline phosphatase enzyme (alkaline phosphatase staining) and mineralization of MSCs (alizarin red staining on day 10).    Results Our results showed early expression of osteopontin and osteocalsin in HSCs coculture with MSCs. These findings support the role of HSCs in induction of osteoblastic differentiation of MSCs. Decrease in the expression of CD90, higher activation in cell alkaline phosphatase enzyme, and mineralization confirmed the results.   Conclusions In the field of human stem cells, our ex vivo findings demonstrated that HSCs can enhance differentiation of MSCs toward osteoblast cells thereby participating in formation of their niche.      Key words : Hematopoietic Stem Cells Mesenchymal Stem Cell Coculture Osteogenesis Osteoblasts 2012 8 01 226 238 http://bloodjournal.ir/article-1-663-en.pdf
38-664 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 HOXB4 overexpression increases cord blood CD34+ hematopoietic stem cells population during ex vivo expansion M. Allahbakhshian Farsani M. Forozandeh Moghadam foroz@modares.ac.ir N. Amirizadeh M. Soleimani A.A. Pourfatholah   Abstract  Background and Objectives Nowadays, cord blood hematopoietic stem cells (HSCs) are well known as a very valuable source of cells for cell therapy purposes. But unfortunately, the insufficient number of them is a limiting factor for their employment in adult bone marrow transplantation. Cord blood HSCs expansion is an approach for alleviating this problem. It has been shown by several studies during in vivo investigations in mouse models that HOXB4 is one of the most effective tools for inducing HSCs self-renewal and thus HSCs expansion. In this study, we tried to enhance self-renewal of cord blood HSCs by HOXB4 overexpression during ex vivo expansion period.    Materials and Methods In this fundamental-applied research, HSCs were transducted by lentiviral vectors containing HOXB4. HSCs were then cultured in IMDM medium containing 10% FBS and early acting cytokines (Flt3L, SCF, Tpo) for 8 days. Thereafter, we evaluated HOXB4 expression by RT-PCR. The CD34+ subpopulation were also examined by flow cytometry.    Results We detected a high level of HOXB4 gene expression in HOXB4 transducted HSCs relative to the control. We observed that HOXB4 over-expression dramatically increases CD34+ subpopulation of HSCs. The increase in the number of CD34+ HSCs is an indicator of HSCs self-renewal during ex vivo expansion period.   Conclusions All in all, in this survey we observed that HOXB4 overexpression markedly increases CD34+ HSCs during ex vivo expansion period. This approach can be very effective for overcoming the limited number of cord blood HSCs for cell therapy purposes.    Key words: Hematopoietic Stem Cells HOXB4 protein، human Lentivirus 2012 8 01 239 250 http://bloodjournal.ir/article-1-664-en.pdf
38-665 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 The effects of aerobic exercise on erythrocyte indices in cancer patients after autologous hematopoietic stem cell transplantation B. Rezaee Seraji brezaee1359@yahoo.com A.A. Ravasi A. Hajifathali R. Soori M. Mahdizadeh M. Amini   Abstract  Background and Objectives Anemia is one of the most common problems in cancer patients. There is a strong correlation between hemoglobin levels and fatigue and quality of life in these patients. The purpose of this study was to determine the effects of aerobic exercise on erythrocyte indices in cancer patients after autologous hematopoietic stem cell transplantation.   Materials and Methods In this applied and quasi experimental study, we evaluated thirteen transplant patients and compared them to an age and sex-matched control group (n=13). The intensity of cardio training was 60-70% of maximum heart rate for 4-6 weeks, 20-30 minutes each day. The variables of this study were RBC, Hct and Hb levels. The blood sample was taken in the first and last day of hospitalization.   Results The results of this study showed that aerobic exercise has significant effects on RBC (p=0.026), Hct (p=0.032) and Hb (p=0.007) levels in cancer patients after autologous hematopoietic stem cell transplantation.    Conclusions These findings suggest that aerobic exercise may lead to an increased production of hematopoietic factors in cancer patients after autologous hematopoietic stem cell transplantation.      Key words : Aerobic Exercise Erythrocyte Indices Autologous Transplantation Hematopoietic Stem Cell 2012 8 01 251 257 http://bloodjournal.ir/article-1-665-en.pdf
38-560 2024-03-28 10.1002
Scientific Journal of Iran Blood Transfus Organ Sci J Iran Blood Transfus Organ 1027-9520 1735-8248 2012 9 3 Application of stem cells for the treatment of hemoglobinopathies S. Rahgozar rahgozar@sci.ui.ac.ir M. Entezar-e-Ghaem M. Abedi F. Montazeri   Abstract  Background and Objectives Hemoglobinopathies are heterogenic hereditary disorders in which mutations lead to abnormal production of hemoglobin chains, or change in the normal sequence of the hemoglobin amino acids. Thalassemia and sickle cell anemia (SCA) are the most prevalent hemoglobinopathies. Patients with the above mentioned disorders often require hypertransfusion regimens. However, continued blood transfusion may contribute to iron overload and consequent organ deterioration, in addition to viral infections. The aim of this review article is to evaluate recent treatment programs regarding these disorders.   Materials and Methods This paper is evaluating the newest protocols applied for the treatment of thalassemia and SCA by reviewing 51 references using Ebsco, Elsevier, Pubmed and OVID databases.    Results Blood transfusion and medical treatments may improve the lifestyle or increase the lifespan of patients with hemoglobinopathies. However, hematopoietic stem cell transplantation (HSCT) is presented as the only curative therapy for thalassemia and SCA. On the other hand, HSCT may contribute to complications such as infertility and gonadal failure, especially in women, chronic graft-versus-host disease (GVHD), and potential secondary malignancies.   Conclusions In spite of the limitations and complications attributed to HSCT, this method is proved to be the most effective way for treatment of hemoglobinopathies. Different cells and novel strategies used to modify transplantation are introduced in this article.    Key words : Hemoglobinopathies Thalassemia Anemia Sickle Cell Disorders Hematopoietic Stem Cells 2012 8 01 258 272 http://bloodjournal.ir/article-1-560-en.pdf