Journal of Iranian Blood Transfusion
فصلنامه پژوهشی خون
bloodj
Medical Sciences
http://bloodjournal.ir
91
journal91
1027-9520
1735-8248
10.66224/bloodj
fa
jalali
1403
7
1
gregorian
2024
10
1
21
3
online
1
fulltext
fa
تاثیر هیپوکسی خفیف بر بنیادینگی سلولهای CD34+ جدا شده از خون بند ناف در همکشتی با سلولهای بنیادی مزانشیمی مغز استخوان انسان
The effect of mild hypoxia on Stemness of CD34+ cells isolated from umbilical cord blood cocultured with human bone marrow mesenchymal stem cells
سلولهاي بنيادي
Stem cells
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">چکید</span></span></span><span lang="AR-SA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">ه</span></span></span><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">سابقه و هدف </span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">خون بند ناف منبعی غنی از سلولهای بنیادی خونساز است که علاوه بر مزایای فراوان، معایب آن شامل تعداد محدود سلولهای بنیادی و پیوندپذیری با تأخیر میباشد. مسلماً شناسایی استراتژیهایی که در کنار افزایش تکثیر سلولهای بنیادی خونساز یا </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HSC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">باعث حفظ بنیادینگی آن شوند، میتواند اثربخشی پیوند را افزایش دهد. هدف از این مطالعه، بررسی شرایط کشت مختلف در شرایط هیپوکسی (غلظت اکسیژن 5%) بر بیان ژنهای </span></span></span></b><b> </b><a name="_Hlk173917034"><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HOXB4</span></span></span></i></b></a><b><i><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">،</span></span></span></i></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">c-Myc </span></span></span></i></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> <i>،</i> </span></span></span></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">Nanog</span></span></span></i></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و</span></span></span></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">SOX2</span></span></span></i></b><b> </b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در سلولهای </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">CD34<sup>+</sup></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> خون بند ناف بود.</span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">مواد و روشها</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در این مطالعه مداخلهای، سلولهای </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">CD34<sup>+</sup></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> خون بند ناف انسان جداسازی گردید و در محیط کشت بدون سرم در حضور ترکیب سیتوکاینی (</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">TPO</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">، </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">FLT3L</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">SCF</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">) با یا بدون حضور سلولهای بنیادی مزانشیمی مغز استخوان (</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">MSC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">) در غلظت اکسیژن 21% و 5% به مدت 7 روز کشت داده شد (در 5 گروه مختلف). در روز 7 بیان ژنهای </span></span></span></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HOXB4</span></span></span></i></b><b><i><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ،</span></span></span></i></b><b> </b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">c-Myc</span></span></span></i></b><b><i><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ،</span></span></span></i></b><b> </b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">Nanog</span></span></span></i></b><b> </b><b><i><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">،</span></span></span></i></b><b> </b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">SOX2</span></span></span></i></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> با </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">PCR</span></span></span></b><b> </b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times=""> Real time</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">مورد بررسی قرار گرفت. آزمایشها در 3 بار مستقل انجام شد. تجزیه و تحلیل آماری دادهها با استفاده از آزمون </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">ANOVA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> انجام و 05/0 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">p<</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> معنادار در نظر گرفته شد.</span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">یافتهها</span></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">بیشترین بیان ژنهای </span></span></span></b><a name="_Hlk171340533"><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HOXB4</span></span></span></i></b></a><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ،</span></span></span></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">c-Myc</span></span></span></i></b><b> </b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، </span></span></span></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">Nanog</span></span></span></i></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و </span></span></span></b><b><i><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">SOX2</span></span></span></i></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در شرایط کشت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HSC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> با </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">MSC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> مغز استخوان در <a name="_Hlk171164767">غلظت اکسیژن 5% </a>بود. نتایج مطالعه افزایش معنادار از نظر آماری در میزان تکثیر و بیان ژنهای بنیادینگی را در غلظت اکسیژن 5% در مقایسه با 21% نشان داد.</span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">نتیجه گیری</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">ترکیب سلولهای بنیادی مزانشیمی مغز استخوان و غلظت اکسیژن 5% باعث افزایش ظرفیت بنیادینگی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HSC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> میشود و شرایطی شبیه به فضای نیچ را فراهم میآورد.</span></span></span></b></span></span></span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:63%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:63%"><span style="font-family:"2 Lotus""></span></span></span></i></span></span></span></span></span></span><br>
</div>
<span style="font-size:11pt"><span style="line-height:29pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="AR-SA" style="font-size:13.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span>
<div dir="rtl" style="text-align: left;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt">Abstract</span></b><b><span style="font-size:12.0pt"></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Background and Objectives</i></b></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">Cord blood (CB) is a rich source of Hematopoietic stem cells (HSCs). Beside the advantage the main disadvantages of CB are the limited number of stem cells and delayed engraftment. Identifying strategies to enhance expansion and maintain stemness of HSCs can improve transplant efficiency. The goal of this study was to examine different culture conditions on <i>HOXB4</i>, <i>c-Myc</i>, <i>Nanog, Sox2</i> gene expression of CB-HSCs.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Materials and Methods</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">In this interventional study, human cord blood CD34<sup>+</sup> HSC, were cultured in the serum-free me­dium supplemented with cytokines (TPO, FLT3L, SCF) with/without bone marrow mesenchymal stem cell (MSC) in 21% O<sub>2</sub> and 5% O<sub>2</sub> for 7 days. In day 7 the expression of, <i>HOXB4</i>, <i>c-Myc, Nanog</i>, <i>Sox2</i> genes were evaluated by Real time PCR. The data analyzed using the ANOVA test and Value < 0.05 were considered statistically significant.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Results</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">Highest rates of <i>HOXB4</i>, <i>c-Myc, Nanog</i>, <i>SOX2</i><sub> </sub>mRNA were seen in coculture of HSC with bone marrow MSC at 5% O<sub>2</sub>. Our findings demonstrated statistically significant increase of expansion and stemness markers in 5% O<sub>2</sub> tension versus 21%.</span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Conclusions </i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">Bone Marrow (BM) -MSC and 5% O<sub>2</sub> combination enhanced stemness of HSC and better mimicked the niche microenvironment conditions.</span></span></span></span></span><br>
<br>
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<span style="font-size:11pt"><span style="line-height:200%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span class="A7" style="font-family:Calibri,sans-serif"><span style="color:black"><span style="font-size:7.0pt"><span style="line-height:200%"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span></span></span></span></span></span>
خون بند ناف, سلولهای بنیادی خونساز, سلولهای بنیادی مزانشیمی, همکشتی, هیپوکسی
Cord Blood, Hematopoietic Stem Cells, Mesenchymal Stem Cell, Coculture, Hypoxia
197
206
http://bloodjournal.ir/browse.php?a_code=A-10-789-4&slc_lang=fa&sid=1
F.
Mohammadali
فاطمه
محمدعلی
9100319475328460025147
9100319475328460025147
No
استادیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
S.
Abroun
سعید
آبرون
Abroun@modares.ac.ir
9100319475328460025148
9100319475328460025148
Yes
استاد دانشگاه تربیت مدرس
A.
Atashi
امیر
آتشی
9100319475328460025149
0009-0007-0584-6047
No
استادیار مرکز تحقیقات سلولهای بنیادی و مهندسی بافت دانشگاه علوم پزشکی شاهرود