Journal of Iranian Blood Transfusion
فصلنامه پژوهشی خون
bloodj
Medical Sciences
http://bloodjournal.ir
91
journal91
1027-9520
1735-8248
10.66224/bloodj
fa
jalali
1403
3
1
gregorian
2024
6
1
21
2
online
1
fulltext
fa
توزیع گروههای آللی HLA-DRB1 در اقوام ایرانی
Investigating the distribution of HLA-DRB1 allelic groups in Iranian ethnic groups
ايمونولوژي
Imunology
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">چکید</span></span></span><span lang="AR-SA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">ه</span></span></span><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">سابقه و هدف </span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">انجام پیوند سلولهای بنیادی خونساز در بیماران هماتولوژیک نیاز به سازگاری دهنده و گیرنده برای آنتیژنهای سازگاری نسجی یا </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> دارد. این مطالعه جهت بررسی فراوانی آللهای </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و تنوع ژنتیکی آنها در برخی اقوام ایرانی صورت گرفت تا در بانک </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ذخیره شده و جهت جستجوی اهداکننده مناسب در پیوند مغز استخوان، مورد استفاده قرار گیرد. </span></span></span></b><b> </b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold""></span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">مواد و روشها</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در این مطالعه توصیفی، با استفاده از روش </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">PCR-SSP</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">، آللهای </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA-DRB1</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> مربوط به اهداکنندگان، در وضوح پایین تعیین گردید. با استفاده از دادههای آزمایشگاهی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و نرمافزار مرکز سپاس مربوط به اطلاعات اهداکنندگان سلولهای بنیادی خونساز سازمان انتقال خون، اطلاعات قومیتی و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> از افراد دارای قومیتهای گیلک، (510 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">n=</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، 70/24 درصد)، لر (465 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">n=</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، 53/22 درصد)، کرد (719 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">n=</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، 84/34 درصد) و عرب (370 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">n=</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">، 93/17 درصد) جمعآوری و تجزیه و تحلیل شد. با روش کایدو، رابطه معناداری بین فراوانی آللها در قومیتها ارزیابی شد (05/0 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">p<</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">). </span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">یافتهها</span></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در گروههای آللی لکوس ژنی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA-DRB1</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA-DRB1*09</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> (6/0 درصد) و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA-DRB1*12</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> (8/0 درصد) و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times=""> HLA- DRB1*08</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">(6/1 درصد) دارای فراوانی پایین بودند. گروههای آللی با فراوانی بالا عبارت بودند از</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times=""> HLA-DRB1*11 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> (7/22 درصد)، </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA-DRB1*03 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> (8/11 درصد)، </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA-DRB1*15 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> (7/11 درصد) و</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA- DRB1*04 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> (1/11 درصد). از بین این گروههای آللی، فراوانی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">9 گروه آللی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA-DRB1</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، در اقوام مورد مطالعه، تفاوت معنادار نشان دادند.</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold""></span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">نتیجه گیری</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">فراوانی تعدادی از آللها در بین اقوام ایرانی تفاوت معنادار داشتند. شناسایی شباهتها و تفاوتها در فراوانی آللهای </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HLA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> بین اقوام ایرانی میتواند علاوه بر گسترش بانک اهداکنندگان، در راستای برنامهریزی تأمین اهداکنندگان سلولهای بنیادی خونساز برای بیماران از اقوام مختلف ایرانی کمککننده باشد. </span></span></span></b></span></span></span></span></span></span></span><br>
<br>
</div>
<div><span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt">Abstract</span></b><b><span style="font-size:12.0pt"></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Background and Objectives</i></b></span></span></span><br>
<span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">This study looked at the frequency of HLA alleles and their genetic diversity in specific Iranian ethnic groups. This is important for the successful transplantation of hematopoietic stem cells. <span lang="AR-SA" dir="RTL" style="font-family:"Traditional Arabic""></span></span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Materials and Methods</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">In this descriptive study, HLA-DRB1 alleles were determined in low resolution level for donors using the PCR-SSP method. By using the HLA lab data and the stem cell donors’ information in Iranian Blood Transfusion Organization, the ethnic and HLA information from the Gilak (n = 510, 24.70%), Lur (n = 465, 22.53%), Kurd (n = 719, 34.84%), and Arab (n = 370, 17.93%) ethnicities were collected and analyzed. The relationship between alleles and ethnicity was examined, and a statistically significant relationship (p < 0.05) between the frequency of alleles in the studied ethnic groups was evaluated using the chi-square test.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Results</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">The low frequency allelic groups related to HLA-DRB1 were HLA-DRB1*09 (0.6%), HLA-DRB1*12 (0.8%), and HLA-DRB1*08 (1.6%). Allelic groups with higher frequency included HLA-DRB1*11 (22.7%), HLA-DRB1*03 (11.8%), HLA-DRB1*15 (11.7%), and HLA-DRB1*04 (11.1%). Among the allelic groups of HLA-DRB1, the frequency of 9 HLA-DRB1 allelic groups showed significant differences in the studied populations.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Conclusions </i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span><span><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">The frequency of several allelic groups showed significant differences among Iranian ethnicities. Identifying similarities and differences in the frequency of HLA alleles among Iranian ethnicities can help not only expand the pool of donors in registries but also assist in planning for providing of hematopoietic stem cell donors for patients from different Iranian ethnic groups.</span></span></span></span></span><br>
<br>
</div>
HLA-DRB1 , قومیت, ایران
HLA-DRB1, Ethnicity, Iran
118
128
http://bloodjournal.ir/browse.php?a_code=A-10-739-3&slc_lang=fa&sid=1
F.
Yari
فاطمه
یاری
f.yari@ibto.ir
9100319475328460024954
9100319475328460024954
Yes
استاد مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
M.
Zaman Vaziri
مریم
زمان وزیری
9100319475328460024955
9100319475328460024955
No
مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
N.
Bagheri
نادیا
باقری
9100319475328460024956
9100319475328460024956
No
مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
A.
Teimourpour
امیر
تیمورپور
9100319475328460024957
9100319475328460024957
No
استادیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
F.
Sabaghi
فاطمه
صباغی
9100319475328460024958
9100319475328460024958
No
مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
F.
Mortezapour Barfi
فرزانه
مرتضی پور برفی
9100319475328460024959
9100319475328460024959
No
مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون