fa جهش‌های طبیعی در نواحی BCP و preC ویروس هپاتیت B در اهداکنندگان خون با هپاتیت B مزمن anti-HBe+ ويروس شناسي Virology پژوهشي Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">چکید</span></span></span><span lang="AR-SA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">ه</span></span></span><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">سابقه و هدف </span></span></span></i></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">جهش‌های مختلف در ژنوم </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HBV</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> در طول عفونت مزمن ایجاد می‌شوند که به تکثیر ویروس مستعد خطا و فشار سیستم ایمنی میزبان نسبت داده شده است. هدف از این مطالعه، تعیین جهش‌ها در نواحی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">BCP</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">preC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ژنوم ویروس هپاتیت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">B</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> درناقلین فاقد علایم و نقش آن در پایداری عفونت هپاتیت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">B</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> بود.</span></span></span></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">مواد و روش‌ها</span></span></span></i></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در این مطالعه توصیفی، با نمونه‌گیری غیر احتمالی آسان در سال 1397 ، تعداد 40 &nbsp;اهداکننده فاقد علایم و مثبت از نظر </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HBsAg</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و آزمایش‌های تأییدی و منفی از نظر</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times=""> anti- HCV</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">anti-HIV</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، از پایگاه انتقال خون تهران وارد مطالعه شدند. بر روی تمامی نمونه‌ها، آزمایش‌های </span></span></span></b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">(</span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">Total</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">) </span></span></span><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">anti- </span></span></span></b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HBc</span></span></span><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، </span></span></span><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HBe Ag</span></span></span><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و</span></span></span><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times=""> anti- </span></span></span></b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HBe </span></span></span><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">ALT</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">AST</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> انجام شد. واکنش </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">Nested-PCR</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> بر روی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">DNA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> استخراج شده انجام و در نهایت تعیین توالی شدند. تحلیل داده‌ها با نرم‌افزار </span></span></span></b><a href="https://hbv.geno2pheno.org"><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times=""><span style="text-decoration:none"><span style="text-underline:none">https://hbv.geno2pheno.org</span></span></span></span></span></b></a><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> بود. </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif""></span></span></span></b></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">یافته‌ها</span></span></span></i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">از 40 نمونه، (8/94%) 38 نفر مرد و</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">(2/5 %) 2 نفر زن با میانگین سنی 6/8 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">&plusmn;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> 6/38 سال بودند. جهش‌ها در (</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">G1764A</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">BCP (A1762T</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، به ترتیب در (50%) 10 و (40%) 8 مورد هم‌چنین جهش </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">T1754G</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> در (15%) 3 نمونه از 20 نمونه </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HBV</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> مثبت و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">HBeAg</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> منفی مشاهده شد. جهش در ناحیه </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">preC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و جایگاه نوکلئوتید </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">G1896A</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> در (60</span></span></span></b><b><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%">٪</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">) 12 مورد یافت شد.</span></span></span></b></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">نتیجه گیری</span></span></span></i></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">بیشترین جهش‌ها در نواحی(</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">G1764A</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">BCP (A1762T</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و (</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">G1896A</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">) </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">Pre-core</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ویروس هپاتیت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">B</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> مشاهده شد که می‌تواند به عنوان یکی از عوامل مؤثر در عدم پاکسازی و حذف ویروس توسط سیستم ایمنی در میزبان و ایجاد عفونت مزمن هپاتیت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span bold="" new="" roman="" style="font-family:" times="">B</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> &nbsp;مطرح باشد.</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif""></span></span></span></b></span></span></span></span></span></span><br> &nbsp;</div> <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt">Abstract</span></b><b><span style="font-size:12.0pt"></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Background and Objectives</i></b></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">Various mutations can occur in the HBV genome during chronic HBV infection due to error-prone viral replication and stress on the host immune system. This study aimed to determine the mutations in the BCP and preC regions of the hepatitis B virus genome in asymptomatic carriers and its role in the persistence of hepatitis B infection.</span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Materials and Methods</i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">In this descriptive study conducted by easy non-probability sampling method in 2017, 40 asymptomatic blood donors positive in terms of HBsAg and confirmatory tests and negative in terms of anti-HCV and anti-HIV tests were included in the study. Anti-HBc, HBe Ag and anti-HBe (Total) tests and ALT and AST tests were performed. Nested-PCR reaction was performed on the extracted DNA and the sequences were determined. Data analysis was done using online software https://hbv.geno2pheno.org.</span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Results</i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">Out of the 40 examined samples (94.8%), 38 were men and 2 (5.2%) women with an average age of 38.6 &plusmn; 8.6. Mutations in (G1764A) and BCP (A1762T) were observed in 10 (50%) and 8 (40%) cases, respectively, and G54T17 mutation was observed in 3 (15%) samples out of 20 HBV positive and HBeAg negative samples. The mutation in the preC region and the nucleotide position A1896 G was found in 12 cases (60%).</span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Conclusions&nbsp; </i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">The highest frequency of mutations was observed in G1764A and BCP which can be considered as one of the effective factors in not clearing and removing the virus by the host&#39;s immune system and causing chronic hepatitis B infection.</span></span></span></span></span><br> <br> &nbsp;</div> کلمات کلیدی: جهش, ویروس هپاتیت B , اهداکنندگان خون Key words: Mutation, Hepatitis B Virus, Blood donors 1 8 http://bloodjournal.ir/browse.php?a_code=A-10-53-8&slc_lang=fa&sid=1 Z. Sharifi زهره شریفی z.sharifi@ibto.ir 9100319475328460024795 9100319475328460024795 Yes استاد مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون A. Yadegari عباس یادگاری 9100319475328460024796 9100319475328460024796 No مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون Z. Paz زهرا پاز 9100319475328460024797 9100319475328460024797 No مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون