Journal of Iranian Blood Transfusion
فصلنامه پژوهشی خون
bloodj
Medical Sciences
http://bloodjournal.ir
91
journal91
1027-9520
1735-8248
10.66224/bloodj
fa
jalali
1402
4
1
gregorian
2023
7
1
20
2
online
1
fulltext
fa
اثر تابش اشعه گاما بر وضعیت متابولیک در فرآوردههای پلاکتی حاصل از پلاسمای غنی از پلاکت حین نگهداری
The effect of gamma-irradiation on platelet metabolic state inPRP-platelet concentrates during storage
انتقال خون
Blood Transfusion
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="font-weight:bold"><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">چکید</span></span></span><span lang="AR-SA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">ه</span></span></span><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">سابقه و هدف </span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">پرتوتابی کنسانتره­های پلاکتی جهت پیشگیری از بیماری پیوند علیه میزبان ناشی از تزریق خون انجام می­شود. اما برخی از مطالعهها حاکی از کاهش پاسخ پس از تزریق و تشدید آسیب­های دوران نگهداری متعاقب پرتوتابی هستند. در این مطالعه، اثر تابش گاما بر وضعیت متابولیک پلاکتی در فرآورده­های پلاکتی حاصله از پلاسمای غنی از پلاکت حین نگهداری بررسی شد.</span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">مواد و روشها</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">این مطالعه تجربی با ۱۰ فرآورده پلاکت انجام شد که در هر 5 نوبت کاری دو فرآورده هم ­گروه از نظر </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif""> ABO</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> و</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">Rh</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> مخلوط و در دو کیسه تقسیم شدند. یکی تحت تابش اشعه گاما(</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">Gy</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">۳۰) و دیگری به عنوان کنترل در نظر گرفته شد. غلظت گلوکز و فعالیت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">LDH</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> به روش رنگ­سنجی و شمارش پلاکت­­ها و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">pH</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> به ترتیب با استفاده از </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">Sysmex-K21</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">pH</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> متر اندازه­گیری شد. </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">Swirling</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> پلاکتی مورد ارزیابی چشمی قرار گرفت. داده­ها با روش آماری کروسکال- والیس و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">Wilcoxon matched-pairs</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> تجزیه و تحلیل و مقادیر 05/0 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">p<</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">معنادار تلقی شد.</span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">یافتهها</span></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">حرکت گردابی در هر دو گروه حفظ شد. کاهش معنادار شمارش پلاکت­ها و مقادیر </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">pH</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> در روزهای ۵ و ۷ نگهداری در هر گروه بدون تفاوت معنادار مشاهده شد(۰۵/۰ </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">p<</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">). به علاوه کاهش سطح گلوکز و افزایش فعالیت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">LDH</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> حین نگهداری مشاهده شد. افزایش فعالیت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">LDH</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> در پلاکت­های اشعه دیده در مقایسه با کنترل در روزهای پنجم(168 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">±</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> 722 در مقابل 135 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">±</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> 536) </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">و هفتم(۱۷۹ </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">± </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">۹۰۸ در مقابل ۱۲۵ </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">± </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">۶۲۳)</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">نگهداری معنادار بود(به ترتی</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">ب 05/0 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">p<</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> و 01/0 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">p<</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">). </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""></span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">نتیجه گیری</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">کیفیت فرآورده­های اشعه دیده در مقایسه با کنترل با کمترین تغییرات متابولیک حفظ میشود. </span></span></span></b></span></span></span></span></span></span></span><br>
</div>
<div dir="rtl" style="text-align: left;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:12.0pt">Abstract</span></b><b><span style="font-size:12.0pt"></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Background and Objectives</i></b></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">Gamma irradiation (GI) of platelet concentrates (PCs) is done to prevent the risk of Transfusion associated graft versus host disease (TA-GVHD). However, some of studies indicate a lower post-transfusion responses and aggravation of platelet storage lesion (PSL) after GI. In this study, the effect of GI on the platelet metabolic status in stored PRP-PCs was investigated.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Materials and Methods</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">in our experimental study, 10 PRP-PC were randomly obtained from Iranian Blood Transfusion Organization (IBTO). Then, two ABO- and D matched PRP-PC units were pooled and splited into two bags, one kept as control while other was gamma-irradiated with 30 Gray. Within 7-day storage, glucose concentration and LDH activity were measured by enzymo-colorimetric method. PLT count and pH were measured using a hematology whole blood analyzer and the pH meter, respectively. Swirling was evaluated by visual assessment. Statistical data were analyzed by Kruskal-Wallis test with Dunn’s multiple comparison test and Wilcoxon Mached-Pair test. P values of less than 0.05 were considered to be significant.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Results</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">Swirling was maintained in both groups throughout the storage period. Platelet count significantly decreased on Days 5 and 7 of storage but there was no significant difference between irradiated and non-irradiated PCs. Glucose decreased whilst LDH activity increased during storage period. The increments of LDH activities were significant on Days 5 (P<.05) and 7 of storage (P<.01) in non-irradiated groups.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Conclusions </i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">The quality of irradiated PCs is well maintained throughout the storage period, with minimal difference in metabolic parameters compared to non-irradiated PLTs.</span></span></span></span></span><br>
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</div>
کلمات کلیدی: اشعه گاما, پلاسمای غنی از پلاکت, بیماری پیوند علیه میزبان
Key words: Gamma Rays, Platelet-Rich Plasma, Graft vs Host Disease
90
100
http://bloodjournal.ir/browse.php?a_code=A-10-835-5&slc_lang=fa&sid=1
F.
Kiani nodeh
فاطمه
کیانی نوده
9100319475328460024116
9100319475328460024116
No
مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
M.
Ghasemzadeh
مهران
قاسم زاده
9100319475328460024117
9100319475328460024117
No
استاد مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
ٍE.H.
Hosseini
احترام السادات
حسینی
e.hosseini10@yahoo.com.au
9100319475328460024118
9100319475328460024118
Yes
دانشیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون