Journal of Iranian Blood Transfusion
فصلنامه پژوهشی خون
bloodj
Medical Sciences
http://bloodjournal.ir
91
journal91
1027-9520
1735-8248
10.66224/bloodj
fa
jalali
1401
12
1
gregorian
2023
3
1
20
1
online
1
fulltext
fa
شرایط محلولسازی گلبول قرمز متراکم جهت دستیابی به آنتیژن Duffy a در آزمایشگاه
Investigating the solubilization conditions of packed red blood cells to obtain Duffy a antigen in the laboratory
ايمونوهماتولوژي
Imunohematology
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">چکید</span></span></span><span lang="AR-SA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">ه</span></span></span><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">سابقه و هدف </span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">یکی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">از</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">آنتی</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">ژن</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">های</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">فرعی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">گروه</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">خونی،</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Duffy</span></span></b><b> </b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">می</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">باشد</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">که</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">شامل</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">زیرگروه</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">های</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">مختلف</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">است</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">. </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">سیستم</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">گروه</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">خونی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Duffy</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> ، از</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">نظر</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">انتقال</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">خون،</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">از</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">اهمیت</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">بالینی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">برخوردار</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">است،</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">لذا</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">شناسایی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">دقیق</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">این</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">آنتی</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">ژن</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">اهمیت</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">به</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">سزایی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">دارد</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">. </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">دراین</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">مطالعه،</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">فرآیند</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">محلول</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">سازی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">غشای</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">گلبول</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">قرمز و</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">خالص</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">سازی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">آنتی</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"></span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">ژن</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">بررسی</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">شد</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">.</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif""></span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">مواد و روشها</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">در این مطالعه تجربی، کیسه گلبول قرمز متراکم با ویژگی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">O<sup>-</sup></span></span></b><b><sup> </sup></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Fy<sup>a+</sup></span></span></b><b><sup> </sup></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">از پایگاه انتقال خون استان تهران، تهیه شده و</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">با به کارگیری دترژانت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Nonidet P-40</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">، اقدام به محلولسازی غشای آنها شد. در مرحله بعد، آنتیژن </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Fy<sup>a</sup></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> با استفاده از روش کروماتوگرافی تمایلی و به کارگیری آنتیبادی اختصاصی این آنتیژن خالصسازی شد. ویژگی پروتئین خالص شده با روش</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">ELISA </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> بررسی شد.</span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">یافتهها</span></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">ویژگی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Fy<sup>a+</sup></span></span></b><b><sup> </sup></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">در گلبولهای قرمز کیسه </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">RBC</span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> با روش آگلوتیناسیون تایید شد. به علاوه، ویژگی آنتیژن تخلیص شده از کیسه یاد شده، با روش </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">ELISA</span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> تایید شد. جذب نوری</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">OD 450 nm </span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> آنتیژن تخلیص شده در روش </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">ELISA</span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> با آنتیبادیهای </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Fy<sup>a</sup></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Fy<sup>b</sup></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">، به ترتیب 137/0 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">±</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:"> 056/1 و 016/0 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">± 197/0 </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">(2 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">n=</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">) بود. </span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">نتیجه گیری</span></span></span></i></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">جهت دستیابی به آنتیژن </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">Fy<sup>a</sup></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">، محلولسازی غشای گلبول قرمز با استفاده از دترژانت غیر یونی </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> Nonidet P-40</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">، قابل انجام بوده و میتوان</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span b="" badr="" style="font-family:">در مطالعههای بعدی از آنتیژن به دست آمده، در زمینههای مختلف از جمله ایمنیزایی و تولید آنتیبادی بهره برد.</span></span></span></b></span></span></span></span></span></span><br>
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<div dir="rtl" style="text-align: left;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt">Abstract</span></b><b><span style="font-size:12.0pt"></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Background and Objectives</i></b></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">One of the antigens of the blood group is Duffy, which includes different subtypes. This system is of clinical importance in terms of blood transfusion, so accurate identification of this antigen is very important. In this study, the process of solubilization of red blood RBC membrane and purification of the Duffy antigen, were investigated.</span></span></span></span></span><br>
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<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Materials and Methods</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">In this experimental study, packed RBCs (O- and Fy<sup>a+</sup>) were obtained from the blood donation center of Tehran province. The membranes of RBCs were solubilized using Nonidet P-40 detergent. In the next step, Fy<sup>a</sup> antigen was purified by affinity chromatography method using a specific antibody against this antigen. The specificity of the purified protein was tested by ELISA technique<span arabic="" dir="RTL" lang="AR-SA" style="font-family:" traditional="">.</span></span></span></span></span></span><br>
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<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Results</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">The Fy<sup>a+</sup> characteristic of red blood cells of the RBC bag was confirmed. In addition, the specificity of the antigen was confirmed by ELISA method. The absorbance levels of the purified antigen (OD450nm) in ELISA method were 1.056 ± 0.137 and 0.197 ± 0.016 by employing the Fy<sup>a</sup> and Fy<sup>b</sup> antibodies, respectively.</span></span></span></span></span><br>
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<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Conclusions </i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">The present research showed that red blood cell membrane solubilization can be done to purify Fy antigen and this antigen may be used in various fields including immunization and antibody production<span arabic="" dir="RTL" lang="AR-SA" style="font-family:" traditional="">.</span></span></span></span></span></span><br>
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<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><i><span style="font-size:9.0pt"></span></i></span></span></span></span></span><br>
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کلمات کلیدی: کروماتوگرافی تمایلی, گلبول قرمز, سیستم گروه خونی دافی
Affinity Chromatography, RBC, Duffy Blood Group system
28
33
http://bloodjournal.ir/browse.php?a_code=A-10-1528-1&slc_lang=fa&sid=1
K.
Abedini
کوثر
عابدینی
9100319475328460023331
9100319475328460023331
No
مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون تهران
F.
Yari
فاطمه
یاری
f.yari@ibto.ir
9100319475328460023332
9100319475328460023332
Yes
استاد مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
S.
Milani
سعیده
میلانی
9100319475328460023333
9100319475328460023333
No
استادیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون