Journal of Iranian Blood Transfusion
فصلنامه پژوهشی خون
bloodj
Medical Sciences
http://bloodjournal.ir
91
journal91
1027-9520
1735-8248
10.61882/bloodj
fa
jalali
1402
4
1
gregorian
2023
7
1
20
2
online
1
fulltext
fa
تغییرات میزان میتوکندریالDNA طی نگهداری در فرآوردههای گلبول قرمز متراکم و گلبول قرمز کم لکوسیت
Changes in the amount of mitochondrial DNA during storage in RBCs and Leukoreduced red blood cells
بانك خون
Blood banking
پژوهشي
Research
<div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><span style="font-weight:bold"><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">چکید</span></span></span><span lang="AR-SA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">ه</span></span></span><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">سابقه و هدف </span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">وجود میتوکندریال</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">DNA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> در فرآوردههای خونی میتواند باعث تغییرات فیزیولوژیک سلولها و در نهایت بروز عوارض در بیماران گردد. هدف از این مطالعه، ارزیابی تأثیر مدت زمان ذخیرهسازی بر روی تعداد نسخه میتوکندریال</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">DNA</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> آزاد</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> در فرآوردههای گلبول قرمز متراکم و گلبولهای قرمز کم لکوسیت بود. </span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">مواد و روشها</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">در یک مطالعه توصیفی مقطعی، تعداد 15 واحد گلبول قرمز متراکم تهیه شده از خون کامل و 15 عدد گلبول قرمز کم لکوسیت وارد مطالعه شدند. پس از ارزیابی پارامترهای هماتولوژیک در هر کیسه با استفاده از روش </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">RT-PCR</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> <a name="_Hlk134352413">تعداد نسخه میتوکندریال</a></span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">DNA</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> آزاد</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">، در فرآوردههای خون در روزهای 0 ، 5 و 35 انجام گردید. پس از جمعآوری دادهها، آزمایش شاپیرو و منویتنی انجام و تجزیه و تحلیل با نرمافزار 2009 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">REST</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> صورت گرفت.</span></span></span></b></span></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">یافتهها</span></span></span></i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">میانگین هموگلوبین در کیسههای خون </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">gr/dL</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> 3/4 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">±</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> 6/17 بود. میانگین پلاکت در کیسههای گلبول قرمز نیز 60/50 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">±</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> 02/132 <a name="_Hlk133667611">هزار در میلیمتر مکعب خون، </a>همچنین میانگین شمارش گلبولهای قرمز نیز 14/0 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">± 07/6 </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">میلیون در هر میکرولیتر خون و میانگین شمارش گلبولهای سفید در کیسه خون نیز 3/2 </span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%">± 98/4 </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">در میکرولیتر بود. تعداد نسخه میتوکندریال</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">DNA</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> آزاد در فرآورده گلبول قرمز متراکم در مقایسه با فرآورده گلبول قرمز کم لکوسیت در روزهای 5 و 35 بیشتر بود. </span></span></span></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">نتیجه گیری</span></span></span></i></b></span></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus"">تعداد نسخه میتوکندریال</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">DNA</span></span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> آزاد در فرآوردههای گلبول قرمز متراکم بیشتر از گلبول قرمز کم لکوسیت بود و ارتباط مستقیمی بین میزان </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">WBC</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> و تعداد نسخه میتوکندریال</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:"Times New Roman Bold","serif"">DNA</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""> آزاد وجود دارد.</span></span></span></b><b><i><span lang="AR-SA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr"></span></span></span></i></b></span></span></span></span></span></span><br>
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<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><font size="3"><font face="IRNazanin"><span dir="LTR" lang="EN-US" style="font-size:12.0pt"><span style="font-family:IRNazanin"><span style="font-weight:bold"></span></span></span></font></font></b></span></span></span></span></span>
<div dir="rtl" style="text-align: left;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><span style="font-size:12.0pt">Abstract</span></b><b><span style="font-size:12.0pt"></span></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Background and Objectives</i></b></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">The mitochondrial DNA in blood products can cause physiological changes in cells and patient complications. This study aims to evaluate the effect of storage time on the copy number of free mitochondrial DNA in products of RBCs and leukoreduced red blood cells.</span></span></span></span></span><br>
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<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Materials and Methods</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">In a cross-sectional descriptive study, 15 units of RBCs and 15 units of leukoreduced red blood cells were evaluated. After determining the hematological parameters and the average blood cell count in each bag, the free mitochondrial DNA copy number was evaluated using the RT-PCR method. The mitochondrial DNA copy number was measured in blood products on days 0, 5 and 35. After data collection, Shapiro and Mann-Whitney tests were performed and the analysis was done using REST 2009 software.</span></span></span></span></span><br>
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<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Results</i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">The average hemoglobin level in the blood bags was 17.6 ± 4.3 gr/dL. The mean platelet count in packed RBC bags was 132.02 ± 50.60<span style="font-family:Symbol">´</span>1000/mm<sup>3</sup> of blood. The mean number of red blood cells was 6.07 ± 0.14 million per microliter of blood and the mean WBC count in the blood bags was 4.98 ± 2.3 per microliter. The number of free mitochondrial DNA copies in the packed RBC product was higher compared to the leukocyte-less RBC products on days 5 and 35.</span></span></span></span></span><br>
<br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif""><b><i>Conclusions </i></b></span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">The free mitochondrial DNA copy number in RBCs bags was higher than leukoreduced RBCs,</span></span></span></span></span><br>
<span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span style="font-family:"Times New Roman","serif"">and a direct relationship between the amount of WBC and the number of free mtDNA copy number was observed.</span></span></span></span></span><br>
</div>
کلمات کلیدی: گلبولهای قرمز, میتوکندریال, ژنها
Key words: RBCs, Mitochondrial, Genes
123
133
http://bloodjournal.ir/browse.php?a_code=A-10-684-11&slc_lang=fa&sid=1
P.
Alavi
پویا
علوی
9100319475328460024131
9100319475328460024131
No
مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
A.
Chegini
آزیتا
چگینی
a.chegini@ibto.ir
9100319475328460024132
9100319475328460024132
Yes
استادیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
Sh.
Samiee
شهرام
سمیعی
9100319475328460024133
9100319475328460024133
No
مربی مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون
M.
Alaie
مستانه
علایی
9100319475328460024134
9100319475328460024134
No
استادیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون