fa نقش پلی‎مورفیسم rs371194629 ناحیه 3’-UTR ژن HLA-G بر استعداد ابتلا به ویروس HTLV-1 در اهداکنندگان خون خراسان رضوی بيماري هاي عفوني Infectious disease پژوهشي Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="font-weight:bold"><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">چکید</span></span></span><span lang="AR-SA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal">ه</span></span></span><span lang="FA" style="line-height:80%"><span style="font-family:Jadid"><span style="font-weight:normal"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">سابقه و هدف </span></span></span></i></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">ویروس</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> HTLV-1 </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">از جمله ویروس‌های منتقله از راه خون می</span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&lrm;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">باشد که در برخی مناطق جهان و در مناطقی از شمال شرق ایران به صورت اندمیک مشاهده می</span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&lrm;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">شود. مولکول</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> HLA-G </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">که به عنوان یک آنتی&lrm;ژن لکوسیتی غیرکلاسیک شناخته می</span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&lrm;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">شود، دارای پلی&lrm;مورفیسم </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">rs371194629</span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> در ناحیه </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">3&rsquo;-UTR</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> خود می&lrm;باشد که ناشی از یک توالی اضافه 14 نوکلئوتیدی است و در مطالعه‌های مختلف تاثیر آن بر ابتلا به عفونت&lrm;های مختلف نشان داده شده است. هدف از این پژوهش، بررسی تاثیر این پلی</span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&lrm;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">مورفیسم بر استعداد ابتلا به ویروس</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> HTLV-1 </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در اهداکنندگان خون خراسان رضوی بود</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">.</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""></span></span></span></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">مواد و روش‌ها</span></span></span></i></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در این مطالعه تحلیلی، 131 اهداکننده خون مبتلا به ویروس</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> HTLV-1 </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">و 131 اهداکننده سالم شرکت داشتند. پس از استخراج</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> DNA </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">از لنفوسیت‌های خون محیطی، ژنوتیپ پلی</span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&lrm;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">مورفیسم</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> rs371194629 </span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&nbsp;با روش </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">PCR-SSP</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> تعیین شد. یافته‌های آماری به کمک آزمون کای‌دو و نرم&lrm;افزار 23</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%"> SPSS </span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&nbsp;تجزیه و تحلیل گردید</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">.</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""></span></span></span></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">یافته‌ها</span></span></span></i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">ژنوتیپ هتروزیگوت </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">DI</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، بیشترین فراوانی را در هر دو گروه بیمار و شاهد داشت. نتایج به دست آمده نشان‌‌دهنده اختلاف معنا&lrm;داری بین فراوانی ژنوتیپ </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">DD</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و استعداد ابتلا به ویروس </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">HTLV-1</span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> بود(03/0 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">p=</span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> ، 11/3-14/1 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">CI=</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> 95% ، 89/1 </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">OR=</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">). بین فراوانی آلل&lrm;های </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">D</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">I</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> و بیماری ارتباطی یافت نشد.</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span style="font-family:"2 Lotus""></span></span></span></b></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i><span lang="FA" style="font-size:9.0pt"><span style="line-height:80%"><span style="font-family:Titr">نتیجه گیری</span></span></span></i></b></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:inter-ideograph"><span style="line-height:80%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">با توجه به نتایج به دست آمده و نیز نتایج دیگران، پلی</span></span></span></b><b><span dir="LTR" lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">&lrm;</span></span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">مورفیسم </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">&nbsp;rs371194629</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">در ناحیه </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">3&rsquo;-UTR</span></span></b><b><span style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> مولکول </span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">HLA-G</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> می&lrm;تواند</span></span></span></b><b> </b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:">یکی از عوامل مؤثر در ابتلا به ویروس</span></span></span></b><b><span dir="LTR" style="font-size:9.0pt"><span style="line-height:80%">HTLV-1</span></span></b><b><span lang="FA" style="font-size:11.0pt"><span style="line-height:80%"><span 2="" lotus="" style="font-family:"> باشد. </span></span></span></b></span></span></span></span></span></span><br> <br> &nbsp;</div> <div dir="rtl" style="text-align: left;"><span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:12.0pt">Abstract</span></b><b><span style="font-size:12.0pt"></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Background and Objectives</i></b></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">The HLA-G antigen is a non-classical HLA class-I with limited polymorphism. Several studies have indicated the role of a 14 bp Indel polymorphism rs371194629 in the 3&#39;-UTR region of the HLA-G in acquisition of HTLV-1 infection. In this study, we investigated the correlation between the genotypes of this polymorphism and HTLV-1 infection in blood donors of Khorasan Razavi province.</span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Materials and Methods</i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">In this analytical study, a total of 131 healthy individuals and 131 HTLV-1 positive patients were analyzed. After DNA extraction, the genotypes of the polymorphism were determined by PCR-SSP method. Statistical analysis was performed with chi-squared test using SPSS version 23 software.<span lang="AR-SA" dir="RTL" style="font-family:"Traditional Arabic""></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Results</i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">The DI was the most frequent genotype in both groups (49.6% of controls and 40.3% of patients). The DD genotype was associated with increased risk of HTLV-1 infection (OR=1.89, 95% CI=1.14-3.11, p=0.03). There was no significant difference between the frequency of D and I alleles in both groups.<span lang="AR-SA" dir="RTL" style="font-family:"Traditional Arabic""></span></span></span></span></span></span><br> <br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><i>Conclusions&nbsp; </i></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times="">Our findings confirm previous results about the significance of HLA-G polymorphisms in susceptibility to infectious diseases including HTLV-1. Further investigations could reveal the underlying mechanisms.<span lang="AR-SA" dir="RTL" style="font-family:"Traditional Arabic""></span></span></span></span></span></span><br> <br> <br> <span dir="RTL"></span></div> HTLV-1 , عفونت, HLA-G HTLV-1, Infection, HLA-G 24 29 http://bloodjournal.ir/browse.php?a_code=A-10-610-5&slc_lang=fa&sid=1 Z. Ganjian Mofrad زینب گنجیان مفرد 9100319475328460023447 9100319475328460023447 No مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون A. Arabkhazaeli علی عرب خزائلی 9100319475328460023448 9100319475328460023448 No مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون F. Mollahoseini فرزاد ملاحسینی 9100319475328460023449 9100319475328460023449 No مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون و اداره کل انتقال خون مشهد M. Shahabi مجید شهابی majshahabi@yahoo.com 9100319475328460023450 9100319475328460023450 Yes استادیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون ـ