fa تغییرات میتوکندریال DNA ، طی نگهداری فرآورده‌های پلاکتی تهیه شده به روش پلاسمای غنی از پلاکت انتقال خون Blood Transfusion پژوهشي Research <div style="text-align: justify;"><span style="font-weight:normal;"><span style="font-family:Jadid;">چکید</span></span><span style="font-weight:normal;"><span style="font-family:Jadid;">ه</span></span><span style="font-weight:normal;"><span style="font-family:Jadid;"></span></span><br> <strong><em><span style="font-family:Titr;"><span style="font-size:9.0pt;">سابقه و هدف </span></span></em></strong><br> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">فعال</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">شدن</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">پلاکت،</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">به دلایل</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">متعدد</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">لحظه</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">نمونه‌گیری</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تا</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">قبل</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تزریق</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">فرآورده</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">رخ</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">می‌دهد. پلاکت‌های</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">فعال شده،</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">میتوکندری‌های</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">فعال</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">را به</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">سمت</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">غشا</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تغییر</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">مکان</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">داده، آن‌ها از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">غشاء</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">جوانه</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">زده</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">و می‌توانند</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">به</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">صورت</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">آزاد</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">ریزش</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">کرده و</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">منجر</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">به</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">آزادشدن</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">واسطه‌های</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">پیش</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">التهابی</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">و میتوکندریال </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">DNA</span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;"> گردند. بدین جهت،</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">میزان میتوکندریال </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">DNA</span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;"> را</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">به</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">عنوان</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">یکی</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">پارامترهای</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">فعالیت</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">پلاکتی</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">در نظرگرفته‌اند. هدف</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">این</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">مطالعه، مقایسه میزان میتوکندریال </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">DNA</span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;"> در</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">طول</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">زمان نگهداری</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">بود.</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;"></span></span></strong><br> <strong><em><span style="font-family:Titr;"><span style="font-size:9.0pt;">مواد و روش‌ها</span></span></em></strong><br> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">در</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">این</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">مطالعه</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تجربی، 22 فرآورده</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">پلاکتی</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;"> PRP </span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">،</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">پس</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">اخذ رضایت‌نامه</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">اهداکنندگان مرد مراجعه‌کننده</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">به</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">مرکز</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">نوآوری</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">سازمان</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">انتقال</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">خون</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">ایران</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">به دست</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">آمد. از</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">این</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">کیسه‌ها</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">در روزهای 1، 3 و 5 نمونه‌گیری</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">شد تا میزان</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تغییرات میتوکندریال</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">DNA </span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">&nbsp;در این فرآورده‌ها</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">توسط</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">روش</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;"> Real time PCR </span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">بررسی</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">شود</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">.</span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;"> نتایج</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">با</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">آزمون آماری </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">paired t-test</span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;"> تجزیه</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">و تحلیل</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">شده</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">ومیزان</span></span></strong> <strong><span dir="LTR"><span style="font-size:9.0pt;">p </span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">کمتر از 05/0 معنادار در نظرگرفته</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">شد</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">.</span></span></strong><br> <strong><em><span style="font-family:Titr;"><span style="font-size:9.0pt;">یافته‌ها</span></span></em></strong><br> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">میزان میتوکندریال</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">DNA </span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">&nbsp;در</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">روز اول افزایش و به تدریج روز</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">3 به 1، 8/0 برابر</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">و</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">در</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">روز 5 به 3 ، 6/0 برابر و</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">در</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">روز</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">5</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">&nbsp;به</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">1</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">، 49/0 برابر</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تغییر یافته و کاهش</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">را</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">نشان</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">داد. این تغییرات در روزهای 3 به 1 به صورت غیر معنادار و در روزهای 5 به 1 به صورت معنادار بود(003/0 </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">P=</span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">).</span></span></strong><br> <strong><em><span style="font-family:Titr;"><span style="font-size:9.0pt;">نتیجه گیری</span></span></em></strong><br> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">میزان میتوکندریال </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">DNA</span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;"> در</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">فرآورده‌های</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تولید</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">شده</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">به</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">روش</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;"> PRP </span></span></strong><strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">، در روز اول افزایش و</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">طی</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">روزهای</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">ذخیره‌سازی</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">تغییر</span></span></strong> <strong><span style="font-family:2 Lotus;"><span style="font-size:11.0pt;">کرده وکاهش یافت. </span></span></strong><br> &nbsp;</div> <div dir="rtl" style="text-align: left;"><strong>Abstract</strong><br> <strong><em>Background and Objectives</em></strong><br> Platelet activation occurs for a variety of reasons from the moment of sampling to that of injection. Activation of the platelets leads to activation of mitochondria. They move toward the membrane, sprouting and shedding freely from it, leading to the release of pro inflammatory mediators and mtDNA. Therefore, mtDNA level is considered as one of the parameters of platelet activity. The aim of this study was to compare mtDNA levels during storage.<br> &nbsp;<br> <strong><em>Materials and Methods</em></strong><br> This experimental study was performed on 22 PRP products. We obtained consent of all donors referring to Iran Innovation Center of Iranian Blood Transfusion Organization. Samples were taken from the PRP bags on day 1, 3 and 5. Then, the fold change of mtDNA was assessed by Real time PCR. The results were analyzed by paired t-test and the P value of less than 0.05 was considered significant.<br> &nbsp;<br> <strong><em>Results</em></strong><br> The rate of mtDNA decreased 0.8-fold on day 3 to 1, 0.6-fold on day 5 to 3, and 0 .49 on day 5 to 1. These changes were significant on days 5 to 1 (p= 0.003) and they were not significant on days 3 to 1 (p= 0.4).<br> &nbsp;<br> <strong><em>Conclusions&nbsp; </em></strong><br> The amount of mtDNA increased on the first day, and then decreased during the storage of&nbsp; PRP products.<br> &nbsp;<br> &nbsp;</div> Real-Time Polymerase Chain Reaction , DNA , میتوکندری, پلاسمای غنی از پلاکت Real-Time Polymerase Chain Reaction, DNA, Platelet-Rich Plasma 27 35 http://bloodjournal.ir/browse.php?a_code=A-10-684-8&slc_lang=fa&sid=1 L. Vakili لیلا وکیلی l.vakili15@gmail.com 9100319475328460023577 9100319475328460023577 Yes مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون A. Chegini آزیتا چگینی a.chegini@ibto.ir 9100319475328460023578 9100319475328460023578 No استادیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون Sh. Samiee شهرام سمیعی shamie@yahoo.com 9100319475328460023579 9100319475328460023579 No مربی مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون M. Shaiegan مژگان شایگان Mojgan.Sh46@gamil.com 9100319475328460023580 9100319475328460023580 No دانشیار مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون