fa بررسی مولکولی عفونت همزمان به ویروس‌های HCV و HBV در مبتلایان به عفونت HIV در استان چهار محال و بختیاری بيماري هاي عفوني Infectious disease پژوهشي Research <div style="text-align: justify;"><span style="font-weight:normal;"><span style="font-family:jadid;">چکید</span></span><span style="font-weight:normal;"><span style="font-family:jadid;">ه</span></span><span style="font-weight:normal;"><span style="font-family:jadid;"></span></span><br> <strong><em><span style="font-family:titr;"><span style="font-size:9.0pt;">سابقه و هدف </span></span></em></strong><br> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">هپاتیت</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">&shy;</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">های</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">ویروسی</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;"> B </span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">و</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;"> C </span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">، از</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">مشکلات</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">درمانی</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">افراد</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">مبتلا</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">به</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">عفونت</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;"> HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> به &shy;شمار</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">می</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">&shy;</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">روند. اهمیت</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">این عفونت‌ها</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">در</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">راه</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">انتقال</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">مشترک</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">آن&shy;ها</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">است</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">و</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">نیز</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">این که بیماری</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">کبد</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">در نتیجه</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">عفونت</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">با </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HCV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HBV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> و عفونت توأم با </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> می&shy;تواند</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">باعث</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">مرگ</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">شود.</span></span></strong><br> <strong><em><span style="font-family:titr;"><span style="font-size:9.0pt;">مواد و روش‌ها</span></span></em></strong><br> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">در یک مطالعه توصیفی ـ مقطعی در سال 1394 ، تعداد 31 نمونه سرم از افراد مراجعه‌کننده به مرکز مشاوره بیماری&shy;های رفتاری مرکز بهداشت استان چهار محال و بختیاری که از نظر ابتلا به </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> با روش وسترن بلات مثبت قطعی تشخیص داده شده بودند، با کسب رضایت ایشان تهیه و از نظر عفونت هم&shy;زمان با ویروس‌های </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HBV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HCV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> به&shy; روش </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">PCR</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> مورد بررسی قرار گرفتند. یافته‌ها به کمک آزمون دقیق فیشر و نرم‌افزار 7 </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">prism</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">، تجزیه و تحلیل شدند. </span></span></strong><br> <strong><em><span style="font-family:titr;"><span style="font-size:9.0pt;">یافته‌ها</span></span></em></strong><br> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">نتایج حاصل از این تحقیق بر روی نمونه&shy;های سرم افراد مبتلا به </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> ، فراوانی هم&shy; زمان عفونت </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HBV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HCV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> در افراد </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> مثبت را 22/3% ، فراوانی هم زمان عفونت </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HBV</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">در افراد </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> مثبت را 45/6% و فراوانی هم زمان عفونت </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HCV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> در مبتلایان به </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> را 5/22% برآورد کرد.</span></span></strong><br> <strong><em><span style="font-family:titr;"><span style="font-size:9.0pt;">نتیجه گیری</span></span></em></strong><br> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">با توجه</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">به</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">راه&shy;های</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">مشترک انتقال</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">ویروس&shy;های </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HCV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> ، </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HBV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> ، تمامی افرادی که از نظر</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> آزمایش می&shy;شوند</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">و</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">یا</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">آلوده</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">به</span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">HIV </span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">&nbsp;هستند</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">بایستی</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">از</span></span></strong> <strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;">نظر ابتلا به ویروس&shy;های هپاتیت </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">B</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> و </span></span></strong><strong><span dir="LTR"><span style="font-size:9.0pt;">C</span></span></strong><strong><span style="font-family:2 lotus;"><span style="font-size:11.0pt;"> نیز بررسی شوند.</span></span></strong><br> <br> &nbsp;</div> <div dir="rtl" style="text-align: left;"><strong>Abstract</strong><br> <strong><em>Background and Objectives</em></strong><br> Viral hepatitis B and C are considered to be a medical problem for people with HIV infection. The importance of these infections is in the shared route of their transmission, and liver disease as a result of infection with HCV, HBV and HIV infection that can lead to death<span dir="RTL">.</span><br> &nbsp;<br> <strong><em>Materials and Methods</em></strong><br> In this cross-sectional descriptive study, a total of 31 serum samples were prepared from patients referring to the Behavioral Diseases Counseling Center in Chaharmahal and Bakhtiari province who were diagnosed with HIV infection by Western blotting method; their consent was taken. The coinfection with HBV and HCV was investigated by PCR method. Data were analyzed by fisher exact test and prism7 software.<br> &nbsp;<br> <strong><em>Results</em></strong><br> The results of this study on serum samples of HIV-infected people showed the prevalence of HCV/HBV coinfection in HIV-positive individuals to be %3.22, the prevalence of HBV infection in HIV-positive individuals %4.45, and the prevalence of HCV infection in HIV infected ones %22.5.<br> &nbsp;<br> <strong><em>Conclusions&nbsp; </em></strong><br> According to the common routes of transmission of HCV, HBV, and HIV viruses, it is essential that all people who are HIV-positive or HIV-infected be screened for hepatitis B and C viruses by highly sensitive diagnostic methods.<br> &nbsp;<br> &nbsp;</div> کلمات کلیدی: عفونت هم زمان, هپاتیت B, هپاتیت C, HIV, تشخیص مولکولی Key words: Coinfection, Hepatitis B, Hepatitis C, HIV, Molecular Diagnostics 301 309 http://bloodjournal.ir/browse.php?a_code=A-10-1003-1&slc_lang=fa&sid=1 Farzaneh Taheri Ghahfarkhi فرزانه طاهری farzaneh42@ymail.com 9100319475328460023932 9100319475328460023932 No مرکز تحقیقات انتقال خون مؤسسه عالی آموزشی و پژوهشی طب انتقال خون و پایگاه منطقه ای انتقال خون شهرکرد E. Tajbakhsh الهه تاج بخش ee_tajbakhsh@yahoo.com 9100319475328460023933 9100319475328460023933 Yes دانشیار دانشکده علوم پایه دانشگاه آزاد اسلامی واحد شهرکرد E. Heidari Soureshjani احسان حیدری سورشجانی ehsanheidari2012@yahoo.com@yahoo.com 9100319475328460023934 9100319475328460023934 No مرکز تحقیقات انتقال خون ـ مؤسسه عالی آموزشی و پژوهشی طب انتقال خون و پایگاه منطقه ای انتقال خون شهرکرد