AU - Khoshnaghsh, F. AU - Kheirandish, M. AU - Deyhim, M.R. TI - Quantification of fetomaternal hemorrhage: a compariative study of the HbF dual staining and RhD single staining by flow cytometry PT - JOURNAL ARTICLE TA - Blood-Journal JN - Blood-Journal VO - 8 VI - 2 IP - 2 4099 - http://bloodjournal.ir/article-1-484-en.html 4100 - http://bloodjournal.ir/article-1-484-en.pdf SO - Blood-Journal 2 AB  -   Abstract  Background and Objectives The quantification of fetal cells in the maternal circulation is an important goal to determine the amount of anti-D for prevention of active immunization of a D-negative mother giving birth to a D-positive baby. The aim of this study was to evaluate two flowcytometric staining technics for determination of fetal erythrocytes in maternal blood and to recognize the best.   Materials and Methods In this experimental study, 34 adult D-negative blood samples were spiked with six serial dilutions of D-positive cord blood (0.125, 0.25, 0.5, 1, 5 and 10%) which was representative of 99.9% of the clinical fetomaternal hemorrhage (FMH) they were stained for flow cytometric analysis. The Fetal Cell Count Kit was used for HbF dual staining and monoclonal anti-D for RhD single staining.   Results A comparison between RhD and HbF percentages and FMH volume in spiking samples was performed. A significant correlation between two different parameters in flowcytometric percentages was observed (anti-HbF versus anti-D, r= 0.897, p CP - IRAN IN - LG - eng PB - Blood-Journal PG - 104 PT - Research YR - 2011