RT - Journal Article T1 - In vitro differentiation of human umbilical vein mesenchymal stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green JF - Blood-Journal YR - 2010 JO - Blood-Journal VO - 7 IS - 1 UR - http://bloodjournal.ir/article-1-381-en.html SP - 34 EP - 40 K1 - Cell Differentiation K1 - Umbilical Veins K1 - Mesenchymal Stem Cells K1 - Hepatocytes K1 - Indocyanine Green AB -   Abstract  Background and Objectives Umbilical cord is one of the major sources of mesenchymal stem cells. In this study, the differentiative potential of human umbilical vein mesenchymal stem cells into hepatocyte-like cells has been investigated by cellular uptake of indocyanine green.    Materials and Methods Human umbilical vein mesenchymal stem cells ( UVMSCs) were incubated for 2 weeks in the medium containing hepatocyte growth factor they were also treated with the medium containing oncostatin M for another 2 weeks. The differentiated cells were analyzed by uptake of indocyanine green (ICG), immunofluorescence analysis, and periodic acid-Schiff (PAS) staining.   Results The differentiating cells showed transition from bipolar fibroblast-like morphology to round or oval shape. Cellular uptake of ICG was detected in differentiated cells. Glycogen storage was determined by PAS staining in differentiated cells which were immunoreactive to albumin.   Conclusions Based on these observations, we can conclude that UVMSCs are able to differentiate into hepatocyte-like cells in vitro and ICG-staining is a useful marker to identify differentiated hepatocytes from human umbilical vein mesenchymal stem cells. � Keywords : Cell Differentiation, Umbilical Veins, Mesenchymal Stem Cells, Hepatocytes, Indocyanine Green�  LA eng UL http://bloodjournal.ir/article-1-381-en.html M3 ER -