TY - JOUR T1 - Evaluation of the two FVIII assay types (clotting and chromogenic) for measuring FVIII activity in cryoprecipitate TT - فعالیت فاکتور VIII درکرایو به دو روش تشکیل لخته و رنگ‌زایی JF - Blood-Journal JO - Blood-Journal VL - 8 IS - 3 UR - http://bloodjournal.ir/article-1-520-en.html Y1 - 2011 SP - 229 EP - 233 KW - C ryoprecipitate Coagulum KW - Factor VIII KW - Iran KW - Methods KW - Hemophilia A N2 -   Abstract  Background and Objectives In Iran, cryoprecipitate is an important plasma product for coagulation factors such as factor VIII (FVIII) to be ensured. As risk of bleeding is associated with FVIII activity, it is important to choose a suitable method for its measurement. In the present study we compared clotting and chromogenic assays on FVIII activity in cryoprecipitate.    Materials and Methods FVIII activity of cryoprecipitate from 48 donors with different blood groups was measured by clotting and chromogenic assays. Then the two assay types were compared with each other.   Results Although the absolute values showed a significant difference, a good correlation was seen between one-stage clot-based and chromogenic assays for measuring FVIII activity in cryo.    Conclusions Although the one-stage clotting assay is a suitable method for measuring FVIII activity, the chromogenic assay has a good correlation with clotting based assay. Also a significant difference was seen between the two methods.  M3 ER -