RT - Journal Article T1 - In vitro evaluation of P-Selectin expression of platelet and platelet aggregation in stored platelet concentrates JF - Blood-Journal YR - 2007 JO - Blood-Journal VO - 4 IS - 3 UR - http://bloodjournal.ir/article-1-175-en.html SP - 205 EP - 213 K1 - Platelet K1 - P-Selectin K1 - Flowcytometery K1 - Platelet aggregation AB -   Abstract  Background and Objectives Platelets have a major role in haemostasis. Platelet concentrates are used in thrombocytopeny and platelet dysfunction disorders. Due to recent studies reporting loss of in vivo function of platelets in platelet concentrate units, we determined the level of surface expression of P-Selectin, conducted platelet aggregation tests and pH measurment to evaluate platelet activation during storage.   Materials and Methods In a cross-sectional study, 100 platelet concentrate units prepared by PRP method were evaluated. 34, 33 and 33 units were taken from the first, second, and third day of storage respectively. For each platelet concentrate unit, pH, CD62P-Selectin and platelet aggregation tests with Arashidonic acid and Ristocetin agonists were conducted.   Results Expression of P-Selectin in third day was significantly higher than first day (p LA eng UL http://bloodjournal.ir/article-1-175-en.html M3 ER -