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:: Volume 13, Issue 4 (Winter 2016) ::
Sci J Iran Blood Transfus Organ 2016, 13(4): 291-303 Back to browse issues page
Evaluation of the repair effects of MSCs cultivated with secretome of Nrf2 engineered MSCs on acute kidney injury in rats
F. Jaleh , F. Amiri , M. Dehghan Harati , M. Habibi Roudkenar , M.A. Jalili
Keywords: Key words: Mesenchymal Stem Cells, Acute Kidney Injury, Conditioned Medium
Full-Text [PDF 654 kb]   (1254 Downloads)     |   Abstract (HTML)  (5064 Views)
Type of Study: Research | Subject: Stem cells
Published: 2016/12/24
Full-Text:   (2047 Views)
    References :  
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Original  Article
 

 
 
 
Sci J Iran Blood Transfus Organ 2017; 13(4): 291-303
 
Evaluation of the repair effects of MSCs cultivated with
secretome of Nrf2 engineered MSCs on acute kidney
injury in rats
 
Jaleh F.1, Amiri F.1, Dehghan Harati M.1, Habibi Roudkenar M.1, Jalili M.A.1
 
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine,Tehran, Iran
 
 
Abstract
Background and Objectives
The decrease in mesenchymal stem cells (MSCs) survival rate after transplantation is a major challenge in MSC-cell-therapy. Hence, it is promising to employ some proper strategies for addressing this problem. This study was conducted to assay the therapeutic effects of MSCs treated with Nrf2-manipulated-MSC conditioned medium in acute kidney injury (AKI)-induced animal models. 
 
Materials and Methods
In an experimental study, recombinant plasmid pcDNA3.1-Nrf2 was transfected into bone marrow-derived MSCs and the resulted conditioned medium was harvested. The MSCs was cultivated with Nrf2-manipulated-derived conditioned medium. The conditioned medium-treated-MSCs were transplanted to AKI-induced rats (each group containing 10 rats) and the therapeutic potentialities of these MSCs were evaluated using biochemical and pathological methods.
Results
Fourteen days after MSCs transplantation, there was a significant difference in BUN decreasing in rat groups injected with conditioned medium-treated-MSCs (48 ± 3.5 mg/dL) in comparison with those injected with normal MSCs (100 ± 9.9 mg/dL) (p < 0.001). The number of observed casts (0.26) in kidney tissue sections of these rat groups were also less than (0.51) the groups transplanted with normal MSCS.
 
Conclusions
Cultivation of MSCs in the presence of Nrf2-manipulated-derived conditioned medium increase the therapeutic effects of these cells in AKI-induced models.
 
Key words: Mesenchymal Stem Cells, Acute Kidney Injury, Conditioned Medium
 
 
 
 
 
 
Received: 26 Jun 2015
Accepted:  5 Oct 2016
 
 
 

Correspondence: Jalili MA., PhD of Medicinal Chemistry. Assistant Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052155; Fax: (+9821) 88601599
E-mail: m.jalili@ibto.ir
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Jaleh F, Amiri F, Dehghan Harati M, Habibi Roudkenar M, Jalili M. Evaluation of the repair effects of MSCs cultivated with secretome of Nrf2 engineered MSCs on acute kidney injury in rats. Sci J Iran Blood Transfus Organ 2016; 13 (4) :291-303
URL: http://bloodjournal.ir/article-1-967-en.html
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Volume 13, Issue 4 (Winter 2016) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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