Abstract

Background and Objectives

Recurrent spontaneous abortion is defined as pregnancy loss before the 20^th week of gestation. In some women with history of consecutive miscarriage, thrombophilic gene mutations lead to blood clots in vessels in placenta, decrease oxygen delivery, and end to fetal loss. This study aimed to determine the association of 807C/T (GPIa gene) and PLA₁/PLA₂ (GPIIIa gene) polymorphisms in RSA.

Materials and Methods

In this case-control study, 50 women with the background of two or more consecutive unexplained abortions and 50 women with at least two live births without a miscarriage were selected in Tehran province and they were examined in Biology Research Center of Islamic Azad University، Zanjan (Iran) in 2014. The 807C/T and PLA₁/PLA₂ polymorphisms were analyzed by Tetra-Primer ARMS-PCR and PCR-RFLP methods, respectively. The results achieved from estimating the genotype of these polymorphisms were analyzed by the SPSS16 and Chi Square.

Results

Compared to the control population, there was an increase in the prevalence of the homozygous 807TT genotype in the case group (p = 0.045), but the prevalence of homozygous PLA₂/PLA₂ genotype decreased in two groups (p = 0.467). We reported the prevalence of 807T/T and PLA₂/PLA₂ genotypes as (0.34 , 0.16) and (0.06 , 0.02) for patients and controls, respectively.

Conclusions

The results of the present study showed that 807C/T polymorphism has a correlation with RPA but there is no significant difference between patient and normal groups in PLA₁/PLA₂.