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Induction of cell death and decreased cell proliferation in acute promyelocytic leukemia cells (NB4) by caffeine
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M. Safa   , D. Bashash , M. Hamidpoor |
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| Keywords: Key words: Acute Promyelocytic Leukemia, Apoptosis, Caffeine, Caspases |
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Full-Text [PDF 409 kb]
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Type of Study: Research |
Subject:
Hematology and Oncology
Published: 2016/01/5
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Full-Text: (3317 Views) |
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Sci J Iran Blood Transfus Organ 2016; 12(4): 331-339
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Induction of cell death and decreased cell
proliferation in acute promyelocytic leukemia
cells (NB4) by caffeine
Safa M.1, Bashash D.2, Hamidpour M.3
1Hematology Department, Cellular and Molecular Research Center, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
2Hematology Department, Faculty of Allied Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Faculty of Allied Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
Prevention, control and treatment of cancer using natural materials currently have been considered as the promising strategy. In this regard, caffeine as a purine alkaloid found in various plants, including coffee, cocoa, cola and tea has recently attracted great interest because of its remarkable multifunctional inhibitory effects on cancers such as leukemia. Regarding the anti-cancer effects of caffeine, we sought to investigate the effectiveness of this methylxanthine alkaloid against NB4 acute promyelocytic leukemia cells.
Materials and Methods
In order to examine the effects of caffeine in APL, NB4 cells were cultured in the presence of different concentrations of caffeine; subsequently, MTT, Caspase 3 activity assay, BrdU cell proliferation assay and quantitative real-time PCR were performed to assess the effect of caffeine on cell viability and the possible mechanisms of inducing cell death.
Results
Evaluation of DNA synthesis and cell survival using BrdU and MTT methods indicated that caffeine reduces proliferation and survival of NB4 cells in a dose and time dependent manner. The results of this study showed that elevation in the dose of caffeine induces caspase-3 activation and increases Bax and p21 gene mRNA expression levels.
Conclusions
Overall, our data illustrate that caffeine inhibits cell proliferation and induces apoptosis in acute promyelocytic leukemia NB4 cells. Thus, it can be concluded that caffeine as a substance in tea may be a useful agent for induction of cell death in malignant cells of patients with acute promyelocytic leukemia.
Key words: Acute Promyelocytic Leukemia, Apoptosis, Caffeine, Caspases
Received: 27 Jul 2014
Accepted: 9 Jun 2015
Correspondence: Safa M., PhD of Hematology & Blood Banking. Assiatant Professor of Hematology Department, Cellular and Molecular Research Center, Faculty of Allied Medicine, Iran University of Medical Sciences.
Postal Code: 1449614535, Tehran, Iran. Tel: (+9821) 86704711; Fax: (+9821) 88622576
E-mail: safa.m@iums.ac.ir
Correspondence: Bashash D., PhD of Hematology & Blood Banking. Assiatant Professor of Hematology Department, Faculty of Allied Medicine, Shahid Beheshti University of Medical Sciences.
Postal Code: 1971653312, Tehran, Iran. Tel: (+9821) 22721150; Fax: (+9821) 22721150
E-mail: david_5980@yahoo.com
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Safa M, Bashash D, Hamidpoor M. Induction of cell death and decreased cell proliferation in acute promyelocytic leukemia cells (NB4) by caffeine. Sci J Iran Blood Transfus Organ 2016; 12 (4) :331-339 URL: http://bloodjournal.ir/article-1-881-en.html
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