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Isolation and characterization of hematopoietic and mesenchymal stem cells derived from human placenta tissue
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A. Omidkhoda   , S. Kaviani , M. Soleimani , M. Nikougoftar Zarif , A. Atashi , N. Ahmadbeigi , M. Jamali , M. Nakhlestani |
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| Keywords: Placenta, Hematopoietic Stems Cells, Mesenchymal Stem Cells |
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Type of Study: Research |
Subject:
Stem cells
Published: 2014/06/22
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Full-Text: (4899 Views) |
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Sci J Iran Blood Transfus Organ 2014; 11(2): 93-102
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Isolation and characterization of hematopoietic and mesenchymal stem cells derived from human
placenta tissue
Omidkhoda A.1, Kaviani S.1, Soleimani M.1, Nikougoftar M.2, Atashi A.1, Ahmadbeigi N.3,
Jamali M.2, Nakhlestani M.2
1Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3Digestive Disease Research Institute, Tehran University of Medical Science, Tehran, Iran
Abstract
Background and Objectives
The low frequency of hematopoietic stem cells in umbilical cord blood is the main factor of its application to be restricted for transplantation. If hematopoietic and mesenchymal stem cells of placenta can be isolated and transplanted with cord blood cells, the problem of cell dose limitation will be solved due to increase in the number of primary hematopoietic stem cells and homing of cells through mesenchymal stem cells. Thus in this study, we tried to isolate the maximum number of hematopoietic and mesenchymal stem cells from placenta by enzymatic methods and evaluate their properties.
Materials and Methods
Five different placenta tissues were treated with collagenase and their red blood cells were omitted with the lysis buffer; then, the remaining cells were studied for the presence of hematopoietic and mesenchymal stem cells.
Results
The result showed that 6.02 ± 0.6 and 4.78 ± 0.8 percent of isolated cells were CD34 + and CD34 + together with CD38 -, respectively. These cells having generated all hematopoietic lineages and adherent stromal cells with the property of mesenchymal stem cells were obtained from the culture of placenta cells.
Conclusions
The presence of a remarkable number of CD34 + cells and mesenchymal stem cells in placenta tissue showed that transplantation of these cells with umbilical cord blood cells can increase the quality of transplantation.
Key words: Placenta, Hematopoietic Stems Cells, Mesenchymal Stem Cells
Received: 13 Jul 2013
Accepted: 5 Nov 2013
Correspondence: Kaviani S., PhD of Hematology. Associate Professor of Faculty of Medical Sciences, Tarbiat
Modares University.
P.O.Box: 14115-331, Tehran, Iran. Tel: (+9821) 82883832; Fax: (+9821) 82884507.
E-mail:
kavianis@modares.ac.ir
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Omidkhoda A, Kaviani S, Soleimani M, Nikougoftar Zarif M, Atashi A, Ahmadbeigi N, et al . Isolation and characterization of hematopoietic and mesenchymal stem cells derived from human placenta tissue. Sci J Iran Blood Transfus Organ 2014; 11 (2) :93-102 URL: http://bloodjournal.ir/article-1-876-en.html
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