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Effects of caspase 3 inhibitor on the survival of platelet concentrate during storage
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R. Shiri   , F. Yari , N. Amirizadeh , A. Gharehbaghian , M. Ahmadinejad , M.R. Tabatabaie |
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| Keywords: Key words: Platelets, Caspase 3, Caspase Inhibitors |
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Full-Text [PDF 332 kb]
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| Abstract (HTML) (10332 Views)
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Type of Study: Research |
Subject:
Hematology
Published: 2013/12/29
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Full-Text: (1239 Views) |
References:
- Leytin V, Freedman J. Platelet apoptosis in stored platelet concentrates and other models. Transfus Apher Sci 2003; 28(3): 285-95.
- Brown SB, Clarke MC, Magowan L, Sanderson H, Savill J. Constitutive death of platelets leading to scavenger receptor-mediated phagocytosis. A caspase-independent cell clearance program. J Biol Chem 2000; 275(8): 5987-96.
- Bertino AM, Qi XQ, Li J, Xia Y, Kuter DJ. Apoptotic markers are increased in platelets stored at 37 degrees C. Transfusion 2003; 43(7): 857-66.
- Leytin V, Allen DJ, Mykhaylov S, Mis L, Lyubimov EV, Garvey B, et al. Pathologic high shear stress induces apoptosis events in human platelets. Biochem Biophys Res Commun 2004; 320(2): 303-10.
- Köhler C, Orrenius S, Zhivotovsky B. Evaluation of caspase activity in apoptotic cells. J Immunol Methods 2002; 265(1-2): 97-110.
- Salvioli S, Ardizzoni A, Franceschi C, Cossarizza A. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett 1997; 411(1): 77-82.
- Shrivastava M. The platelet storage lesion. Transfus Apher Sci 2009; 41(2): 105-13.
- Shcherbina A, Remold-O'Donnell E. Role of caspase in a subset of human platelet activation responses. Blood 1999; 93(12): 4222-31.
- Li J, Xia Y, Bertino AM, Coburn JP, Kuter DJ. The mechanism of apoptosis in human platelets during storage. Transfusion 2000; 40(11): 1320-9.
- Plenchette S, Moutet M, Benguella M, N'Gondara JP, Guigner F, Coffe C, et al. Early increase in DcR2 expression and late activation of caspases in the platelet storage lesion. Leukemia 2001; 15(10): 1572-81.
- Piguet PF, Kan CD, Vesin C. Thrombocytopenia in an animal model of malaria is associated with an increased caspase-mediated death of thrombocytes. Apoptosis 2002; 7(2): 91-8.
- Cohen Z, Wilson J, Ritter L, McDonagh P. Caspase
inhibition decreases both platelet phosphatidylserine exposure and aggregation: caspase inhibition of platelets. Thromb Res 2004; 113(6): 387-93.
- Cohen Z, Gonzales RF, Davis-Gorman GF, Copeland JG, McDonagh PF. Thrombin activity and platelet microparticle formation are increased in type 2 diabetic platelets: a potential correlation with caspase activation. Thromb Res 2002; 107(5): 217-21.
- Maekawa Y, Yagi K, Nonomura A, Kuraoku R, Nishiura E, Uchibori E, et al. A tetrazolium-based colorimetric assay for metabolic activity of stored blood platelets. Thromb Res 2003; 109(5-6): 307-14.
- Ohto H, Nollet KE. Overview on platelet preservation: better controls over storage lesion. Transfus Apher Sci 2011; 44(3): 321-5.
- Piguet PF, Vesin C, Da Kan C. Activation of platelet caspases by TNF and its consequences for kinetics. Cytokine 2002; 18(4): 222-30.
- Wadhawan V, Karim ZA, Mukhopadhyay S, Gupta R, Dikshit M, Dash D. Platelet storage under in vitro condition is associated with calcium-dependent apoptosis-like lesions and novel reorganization in platelet cytoskeleton. Arch Biochem Biophys 2004; 422(2): 183-90.
- Perrotta PL, Perrotta CL, Snyder EL. Apoptotic activity in stored human platelets. Transfusion 2003; 43(4): 526-35.
- Skripchenko A, Myrup A, Thompson-Montgomery D, Awatefe H, Moroff G, Wagner SJ. Periods without agitation diminish platelet mitochondrial function during storage. Transfusion 2010; 50(2): 390-9.
- Leytin V. Apoptosis in the anucleate platelet. Blood Rev 2012; 26(2): 51-63.
- Cohen Z, Davis-Gorman G, McDonagh PF, Ritter L. Caspase inhibition of platelet activation. Blood Coagul Fibrinolysis 2008; 19(4): 305-9.
- Lai M, Rumi C, D'Onofrio G, Puggioni PL, Menichella G, Candido A, et al. Phosphatidylserine exposure in platelet concentrates during the storage period: differences between the platelets collected with different cell separators. Transfus Apher Sci 2002; 27(3): 239-45.
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Sci J Iran Blood Transfus Organ 2014; 10(4): 319-325
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Effects of caspase 3 inhibitor on the survival of platelet concentrate during storage
Shiri R.1, Yari F.1, Amirizadeh N.1, Gharehbaghian A.2,3, Ahmadinejad M.1, Tabatabaie M.R.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Paramedical Faculty, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Pediatric Congenital Hematologic Disorders Research Center of Shahid Beheshti University of Medical Sciences,Tehran, Iran
Abstract
Background and Objectives
The time for platelet concentrate storage is 3 to 5 days. One of the limitations for platelet storage was storage lesion in which they lose their viability and undergo apoptosis. Studies show that apoptosis occurs in some anucleated cells such as platelets. In this study, the caspase 3 inhibitor was employed in platelet concentrates to obtain platelets with higher survival.
Materials and Methods
After preparation of platelet concentrates, caspase3 inhibitor (16 μM) was injected to the test bags. These bags along with control bags which lacked the inhibitor were stored at 22 in shaker incubator for 7 days. Samples from these bags were obtained and the activity of caspase3 and the survival levels of platelets were analyzed.
Results
In the presence of caspase3 inhibitor, the activity of caspase3 in platelets decreased compared to the control group. Additionally, this study showed that the survival of platelets decreased during storage but in the presence of caspase3 inhibitor the survival of platelet concentrate was significantly higher than that of the control group (p<0.05).
Conclusions
Injection of caspase3 inhibitor to the platelet concentrate can reduce apoptosis and therefore increase their survival.
Key words: Platelets, Caspase 3, Caspase Inhibitors
Received: 18 Aug 2012
Accepted: 14 Aug 2013
Correspondence: Yari F., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052237; Fax: (+9821) 88601555
E-mail: f.yari@ibto.ir
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Shiri R, Yari F, Amirizadeh N, Gharehbaghian A, Ahmadinejad M, Tabatabaie M. Effects of caspase 3 inhibitor on the survival of platelet concentrate during storage . Sci J Iran Blood Transfus Organ 2014; 10 (4) :319-325 URL: http://bloodjournal.ir/article-1-837-en.html
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