Wiwanitkit V. Phylogenetic tree of hemoglobin Q disorders. The Internet Journal of Hematology 2005; 2(1). Available from: http://ispub.com/IJHE/2/1/4252.
Lorkin PA, Charlesworth D, Lehmann H, Rahbar S, Tuchinda S, Eng LI. Two haemoglobins Q, Alpha-74(EF3) and alpha-75 (EF4) aspartic acid to histidine. Br J Haematol 1970;19(1): 117-25.
Abraham R, Thomas M, Britt R, Fisher C, Old J. Hb Q-India: an uncommon variant diagnosed in three Punjabi patients with diabetes is identified by a novel DNA analysis test. J Clin Pathol 2003; 56(4): 296-9.
Dash S, Huisman TH. Hemoglobin-Q- India (64(E13) Asp-His) and beta thalasemia: a case report from Punjab (North India). Eur J Haematol 1988; 40(3): 281.
Desai D, Parmar C, Dhanani H, Patel RZ, Master DC. A rare case of co-existent Hb Q India-Beta thalassemia trait. The Internet Journal of Hematology 2007; 3(1). Available from: http:// archive. ispub. com/ journal/ the-internet – journal -f-hematology/volume-3-number -1/ a- rare-case-of-co-existent-hb-q-india-beta-thalassemia-trait.html.
Qin WB, Baysal E, Wong KF, Molchanova TP, Pobedimskaya DD, Sharma S, et al. Quantities of alpha Q chain variants in heterozygotes with and without a concomitant beta-thalassemia trait. Am J Hematol 1994; 45(1): 91-3.
Felice AE, Webber BB, Uuisman TH. Alpha-
thalassemia and the production of different alpha chain variants in heterozygotes. Biochem Genet 1981; 19(5-6): 487-98.
Khatami S, Dehboneh SR, Sadeghi S, Mirzazadeh R, Saeedi P, Bayat P, et al. Globin chain synthesis is a useful complementry tool in the differential diagnosis of thalassemias. Hemoglobin 2007; 31(3): 333-41.
International Committee for Standardization in Hematology. Simple electrophoretic system for presumptive identification of abnormal hemoglobins. By the International Committee for Standardization in Hematology. Blood 1978; 52(5): 1058-64.
Rahimi Z, Rezaei M, L Nagel R, Muniz A. Molecular and hematologic analysis of hemoglobin Q-Iran and hemoglobin Setif in Iranian families. Arch Iran Med 2008; 11(4): 382-6.
Rahimi Z, Akramipour R, Vaisi-Raygani A, Nagel RL, Muniz A. An Iranian child with hemoglobin Q-Iran [alpha75 (EF4) Asp-->His]/-alpha3.7 kb/IV SII.1 G-->A: first report. J Pediatr Hematol Oncol 2007; 29(9): 649-51.
Rahimi Z, Muniz A, Mozafari H. Abnormal hemoglobins among Kurdish population of Western Iran: hematological and molecular features. Mol Biol Rep 2010; 37(1): 51-7.
Yadav AK. Comparative analysis of protein structure of common Hb Q variants. Indian J Pathol Microbiol 2010; 53(4): 696-8.
Case Report
Sci J Iran Blood Transfus Organ 2014; 10(4): 411-418
Hemoglobin Q-Iran and the importance of using suitable laboratory screening methods-Case report
Khatami Sh.1, Rouhi Dehnabeh S.1, Najmabadi H.2
1Pasteur Institute of Iran, Tehran, Iran 2Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran
Abstract Background and Objectives
Using suitable laboratory methods for the diagnosis of hemoglobinopathies in laboratories not equipped with automatic systems is essential. The correct diagnosis of hemoglobin Q-Iran (α75Asp → His) is important.
Case
A 33-year-old woman was referred to the national reference globin chain biosynthesis laboratory. The patient's globin chains chromatogram indicated an unknown peak after α globin chain peak. Direct conventional sequencing revealed single G to C missense mutation in the a-globin gene. The genetic analysis led to the identification of a rare hemoglobin variant.
Conclusions
Medical diagnosis laboratories not equipped with modern automatic systems must run solubility test and hemoglobin electrophoresis on citrate agar and cellulose acetate for definite detection of Hb Q-Iran; otherwise, it can be regarded as a cause of misdiagnosis with hemoglobin S.
Key words: hemoglobin Q, Hemoglobinopathies, screening, Iran
Received: 16 Jan 2013 Accepted: 15 Jul 2013
Correspondence: Rouhi Dehnabeh S. DMT. Pasteur Institute of Iran. No. 358, 12th Farwardin Ave, Jomhhoori St., Pasteur St.
Postal Code: 1316943551, Tehran, Iran. Tel: (+9821) 66402770; Fax: (+9821) 66402770
E-mail: rouhisoudabeh@yahoo.com