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Isolation and expansion of Mesenchymal Stem Cells from placenta
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F. Oubari   , M. Nikougoftar Zarif , N. Amirizadeh , M. Shaiegan , K. Atarodi , M. Nakhlestani , M. Mirzamoradi , R. Fadai , Kh. Golzade |
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| Keywords: Mesenchymal Stem Cells, Placenta, Cell Therapy |
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Type of Study: Research |
Subject:
Stem cells
Published: 2013/10/7
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Full-Text: (3296 Views) |
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Sci J Iran Blood Transfus Organ 2013; 10(3): 222-230
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Isolation and expansion of Mesenchymal Stem
Cells from placenta
Oubari F.1, Nikougoftar Zarif M.1, Amirizadeh N.1, Shaiegan M.1, Atarodi K.1, Nakhlestani M.1,
Mirzamoradi M.2,3, Fadai R.1, Golzade Kh.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Shahid Beheshti University of Medical Sciences, Tehran, Iran
3Mahdieh Educational Hospital, Tehran, Iran
Abstract
Background and Objectives
Mesenchymal stem cells are fibroblasts with the capacity of proliferation, colonogenesis, and differentiation to the mesodermal cells that are known as the source for cell therapy purposes. These cells are present in some adult and embryonic tissues in which the bone marrow serves as the main source of human mesenchymal stem cells. Because of some limitations in using the bone marrow, in this study we established a method for isolation of mesenchymal stem cells from placenta and characterized their properties.
Materials and Methods
In this experimental study, we obtained 3 placenta tissues from informed consent mothers after normal vaginal delivery. Mesenchymal stem cells were isolated from the placenta, cultured, and were then examined for their proliferation, colonogenesis, immunophenotyping and differentiation capacities from passages 3 and 7.
Results
Our results showed the high capacity of proliferation and colonogenesis of placental derived mesenchymal stem cells. Immunophenotyping confirmed more than 95% purity of isolated cells; their surface antigen expression showed the phenotypical properties like those of bone marrow derived mesenchymal stem cells. The cells had the osteocytic and adipocytic differentiation capacity.
Conclusions
In this study we successfully isolated mesenchymal stem cells from placenta and cultured them without any alteration in their capacities; consequently, we found out that we can isolate and expand these cells as an alternative source for cell therapies.
Key words: Mesenchymal Stem Cells, Placenta, Cell Therapy
Received: 29 May 2012
Accepted:30 Dec 2012
Correspondence: Nikougoftar Zarif M., PhD of Hematology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601575; Fax: (+9821) 88601576
E-mail: Nikougoftar@ibto.ir
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Oubari F, Nikougoftar Zarif M, Amirizadeh N, Shaiegan M, Atarodi K, Nakhlestani M, et al . Isolation and expansion of Mesenchymal Stem Cells from placenta. Sci J Iran Blood Transfus Organ 2013; 10 (3) :222-230 URL: http://bloodjournal.ir/article-1-791-en.html
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