[Home ] [Archive]   [ فارسی ]  
:: Main :: About us :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Subscription::
News& Events::
Contact us::
Site Facilities::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
Indexing
                        
..
:: Volume 10, Issue 2 (Summer 2013) ::
Sci J Iran Blood Transfus Organ 2013, 10(2): 103-111 Back to browse issues page
Proliferation promoting and apoptosis inhibiting effect of aminated PS nanofibers on cord blood hematopoietic stem cells
F. Sabaghi , K. Shams Asanjan , A.A. Movasaghpour , N. Amirizadeh , M. Nikougoftar , N. Bagheri , P. Abbasi
Abstract:   (11305 Views)

 

  Abstract

 Background and Objectives

 Umbilical cord blood is a promising alternative source of hematopoietic stem/progenitor cells (HSPCs) for allogeneic hematopoietic stem cell transplantation. Although the use of UCB for transplantation has plentiful advantages in comparison with other cell sources, the low number of HSPCs led to cell delayed recovery. Therefore, ex vivo expansion of HSPC before transfusion could induce faster cell recovery.

  

 Materials and Methods

 CD34 positive cells were positively enriched using MidiMACS system and cultured on aminated PS nanofibers, aligned PCLs, and random PCLs. Cell expansion and quantification of cell apoptosis were carried out in expanded cell populations in different conditions by the flowcytometric method.

  

 Results

 This study indicated significantly increased expansion of CD34+ cells and declined apopotosis in PS-cultured cells compared to the same cells on 2-D control culture system (p< 0.05). There were no significantly differences in expansion of CD34+ cells on aligned and random PCL scaffolds than conventional system.

  

 Conclusions

 This study demonstrated that aminated PS nanofibers has positive effects on the expansion of CD34+ cells compared to 2D culture system by increasing cell expansion and cell apopotosis inhibition. However, aligned and random PCL nanofibers did not show supportive effects on cell proliferation.

 

Keywords: Key words: Cord Blood, Hematopoietic Stem Cells, CD34 Antigens
Full-Text [PDF 498 kb]   (1865 Downloads) |   |   Full-Text (HTML)  (1141 Views)  
Type of Study: Research | Subject: Imunology
Published: 2014/04/19
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sabaghi F, Shams Asanjan K, Movasaghpour A, Amirizadeh N, Nikougoftar M, Bagheri N et al . Proliferation promoting and apoptosis inhibiting effect of aminated PS nanofibers on cord blood hematopoietic stem cells. Sci J Iran Blood Transfus Organ 2013; 10 (2) :103-111
URL: http://bloodjournal.ir/article-1-783-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 10, Issue 2 (Summer 2013) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
Persian site map - English site map - Created in 0.05 seconds with 39 queries by YEKTAWEB 4645