|
The influence of Low Density Lipoprotein on vein thrombus formation
|
M. Hamidpour   , A.A. Kadem maboodi , F. Sigarchin taghizadeh , M. Janmaleki , S.B. Seyed Alikhani |
|
|
| Keywords: Key words: Atherosclerosis, Thrombus, Low Density Lipoprotein Cholesterol |
|
|
Full-Text [PDF 441 kb]
(1800 Downloads)
| Abstract (HTML) (6047 Views)
|
Type of Study: Research |
Subject:
Hematology
Published: 2014/09/28
|
|
|
|
|
|
|
Full-Text: (1757 Views) |
References:
- Ross R. The pathogenesis of atherosclerosis: a perspective for the 1990s. Nature 1993; 362(6423): 801-9.
- Rauch U, Osende JI, Fuster V, Badimon JJ, Fayad Z, Chesebro JH. Thrombus formation on atherosclerotic plaques: Pathogenesis and clinical consequences. Ann Intern Med 2001; 134(3): 224-38.
- Fuster V. Mechanisms of arterial thrombosis: foundation for therapy. Am Heart J 1998; 135(6 Pt 3 Su): S361-6.
- Ananyeva NM, Kouiavskaia DV, Shima M, Saenko EL. Intrinsic pathway of blood coagulation contributes to therbogenicity of atherosclerotic plaque. Blood 2002; 99(12): 4475-85.
- Libby P. Changing concepts of atherogenesis. J Intern Med 2000; 247(3): 349-58.
- Delafontanine P. Growth factors and vascular smooth muscle cell growth responses. Eru Heart J 1998; 19 Suppl G: G18-22.
- Haserück N, Erl W, Pandey D, Tigyi G, Ohlmann P, Ravanat C, et al. The plaque lipid lysophosphatidic acid stimulates platelet activation and platelet-monocyte aggregate formation in whole blood: involvement of P2Y1 and P2Y12 receptors. Blood 2004; 103(7): 2585-92.
- Váradi K, Elödi S. Formation and functioning of the factor IXa-VIII complex on the surface of endothelial cells. Blood 1987; 69(2): 442-5.
- Björkerud S, Björkerud B. Apoptosis is abundant in human atherosclerotic lesion; especially in inflammatory cells ( macrophages and T cells), and may contribute to the accumulation gruel and plaque instability. Am J Pathol 1996; 149(2): 367-80.
- Block LH, Knorr M, Vogt E, Locher R, Vetter W, Groscurth P, et al. Low density lipoprotein causes general cellular activation with increased phosphatidylinositol turnover and lipoprotein catabolism. Proc Natl Acad Sci U S A 1988 ; 85(3): 885-9.
- Ardlie NG, Selley ML, Simons LA. Platelet activation by oxidatively modified low density lipoproteins. Atherosclerosis 1989; 76(2-3): 117-24.
- Havel RJ, Eder HA, Bragdon JH. The distribution and chemical composition of ultra centrifugally separated lipoproteins in humans serum. J Clin Invest 1955; 34(9): 1345-53.
- Holvoet P, Mertens A, Verhamme P, Bogaerts K, Beyens G, Verhaeghe R, et al. Circulating oxidized LDL is a useful marker for identifying patients with coronary artery disease. Arterioscler Thromb Vasc Biol
2001; 21(5): 844-8.
- Weidtmann A, Scheithe R, Hrboticky N, Pietsch A, Lorenz R, Siess W. Mildy oxidized LDL induces platelet aggregation through activation of phospholipase A2. Arterioscler Thromb Vasc Biol 1995; 15(8): 1131-8.
- Hackeng CM, Relou IA, Pladet MW, Gorter G, van Rijn HJ, Akkerman JW. Early platelet activation by low density lipoprotein via p38MAP kinase. Thromb Haemost 1999; 82(6): 1749-56.
- van Willigen G, Gorter G, Akkerman JW. LDLs increase the exposure of fibrinogen binding sites on platelets and secretion of dense granula. Arterioscler Thromb 1994; 14(1): 41-6.
- Koller E, Volf I, Gurvitz A, Koller F. Modified low-density lipoproteins and high-density lipoproteins. From investigation tools to real in vivo players. Pathophysiol Haemost Thromb 2006; 35(3-4): 322-45
- Hackeng CM, Huigsloot M, Pladet MW, Nieuwenhuis HK, van Rijn HJ, Akkerman JW. Low-density lipoprotein enhances platelet secretion via integrin-alphaIIbbeta3-mediated signaling. Arterioscler Thromb Vasc Biol 1999; 19(2): 239-47.
- Schmid W, Assinger A, Lee A, Bielek E, Koller E, Volf I. Platelet-stimulating effects of oxidized LDL are not attributable to toxic properties of the lipoproteins. Thromb Res 2008; 122(5): 630-9.
- Chen R, Chen X, Salomon RG, McIntyre TM. Platelet activation by low concentrations of intact oxidized LDL particles involves the PAF receptor. Arterioscler Thromb Vasc Biol 2009; 29(3): 363-71.
- Wang H, Wang ZH, Kong J, Yang MY, Jiang GH, Wang XP, et al. Oxidized low-density lipoprotein-dependent platelet-derived microvesicles trigger procoagulant effects and amplify oxidative stress. Mol Med 2012; 18: 159-66.
- Chen K, Febbraio M, Li W, Silverstein RL. A specific CD36-dependent signaling pathway is required for platelet activation by oxidized low-density lipoprotein. Circ Res 2008; 102(12): 1512-9.
- Leroyer AS, Tedgui A, Boulanger CM. Role of microparticles in atherothrombosis. J Intern Med 2008; 263(5): 528-37.
- Stellos K, Sauter R, Fahrleiner M, Grimm J, Stakos D, Emschermann F, et al. Binding of oxidized low-density lipoprotein on circulating platelets is increased in patients with acute coronary syndromes and induces platelet adhesion to vascular wall in vivo--brief report. Arterioscler Thromb Vasc Biol 2012; 32(8): 2017-20.
|
Sci J Iran Blood Transfus Organ 2014; 11(3): 221-229
|
The influence of Low Density
Lipoprotein on vein thrombus formation
Hamidpour M.1, Khadem Maboudi A.A.1, Sigarchian Taghizadeh F.2,
Janmaleki M.3, Seyed Alikhani S.B.1
1Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Council of Dental Ministry of Health and Medical Education, Tehran, Iran
3Medical Nano-Technology & Tissue Engineering Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
There are several evidences that show lipoproteins especially Low Density Lipoprotein (LDL) and platelets play an important role in the pathogenesis of atherosclerotic plaque and thrombosis. The important issue is how lipids induce primary clot formation and detect primary thrombosis.
Materials and Methods
The present cross-sectional study evaluated 42 of hyper-cholesterolmias who had serum cholesterol levels above the normal range (200 mg/dL; based on the Iranian serum cholesterol reference) and 14 healthy volunteers as the normal control. The serum LDLs of the samples were isolated and oxidized; then, the function of platelet after being affected by natural LDL (N-LDL) and oxidized LDL (OX-LDL) was observed. Finally, the data were statistically analyzed using SPSS version 16 software and the student T-test.
Results
The serum ox-LDL was detected in 234 nm, the absorbance from 0.45 in LDL turned to 0.98 in OX-LDL, (p < 0.0008). Results in our study indicated that, in contrary to N-LDL, OX-LDL induced increased platelet aggregation in aggregometry method, respectly 11% platelet agggragation with N-LDL and 45% with OX-LDL. Flowcytometry analysis of platelet function indicated that; in the vicinity of N-LDL confirm the only 8% of platelets were binding with fibrinogen, whereas 82% of platelets binding with 20 mg/mL of OX-LDL.
Conclusions
The study shows that LDLs modified by serum lipooxidants and exposed to OX-LDL may induce platelet activity and aggregation followed by the increase risk of thrombosis and cardiovascular diseases.
Key words: Atherosclerosis, Thrombus, Low Density Lipoprotein Cholesterol
Received: 18 Jun 2013
Accepted: 21 Jan 2014
Correspondence: Hamidpour M., PhD of Hematology. Assistant Professor of Faculty of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences.
P.O.Box: 19716-53383, Tehran, Iran. Tel: (+9821) 2272504; Fax: (+9821) 22721150
E-mail: mohsenhp@sbmu.ac.ir
|
|
| Send email to the article author |
|
|
|
| Add your comments about this article |
|
|
|
|
Hamidpour M, Kadem maboodi A, Sigarchin taghizadeh F, Janmaleki M, Seyed Alikhani S. The influence of Low Density Lipoprotein on vein thrombus formation. Sci J Iran Blood Transfus Organ 2014; 11 (3) :221-229 URL: http://bloodjournal.ir/article-1-760-en.html
|
.jpg)
.png)
