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:: Volume 7, Issue 2 (Summer 2010) ::
Sci J Iran Blood Transfus Organ 2010, 7(2): 94-100 Back to browse issues page
Phylogenetic analysis of HIV-1 pol gene (RT sequences) among Iranian HIV infected patients
K. Baesi , M. Ravanshad , S.Y. Hosseini , M. Haji Abdolbaghi , K. Shahzamani
Abstract:   (14705 Views)

  Abstract

 Background and Objectives

 Human immunodeficiency virus (HIV) is the retrovirus that causes the disease known as AIDS. In the current study, we examined the genetic diversity of HIV-1 strains circulating in Iran.

 

 Materials and Methods

 Sequencing and phylogenetic analysis of human immunodeficiency virus type 1 (HIV-1) pol gene is a useful method for subtyping strains of HIV-1. HIV-1 RNA was derived from plasma of 24 infected patients in Imam Khomeini Hospital in Tehran. RT Nested-PCR was developed and the product was extracted from agarose gel. The products were sequenced and phylogenetic analysis by Mega 4 software was constructed.

 

 Results

 The developed PCR method successfully amplified the specific sequence. The sequence and phylogenetic analysis showed subtypes A1 and B in 14 and 10 patients, respectively.

 

 Conclusions

 The results indicated intravenous drug users infected with HIV-1 subtype A1, and hemophiliacs infected with HIV-1 subtype B. The results also showed that the subtype A1 is the most prevalent in HIV infected patients. Such information can be useful in intervention strategies and control programs.

  

 Key words: HIV-1, Iran , Genetic Diversity

 

Keywords: HIV-1, Iran, Genetic Diversity
Full-Text [PDF 269 kb]   (2563 Downloads)    
Type of Study: Research | Subject: Infectious disease
Published: 2014/07/23
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Baesi K, Ravanshad M, Hosseini S, Haji Abdolbaghi M, Shahzamani K. Phylogenetic analysis of HIV-1 pol gene (RT sequences) among Iranian HIV infected patients. Sci J Iran Blood Transfus Organ 2010; 7 (2) :94-100
URL: http://bloodjournal.ir/article-1-396-en.html


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Volume 7, Issue 2 (Summer 2010) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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