Prevalence of hepatitis B virus genotypes to be determined by sequencing in blood donors in Kerman, Esfahan and Yazd
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Z. Sharifi , A. Gharehbaghian , M. Noroozi |
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Abstract: (17972 Views) |
Abstract Background and Objectives Hepatitis B is one of the major diseases that has the potential to be transmitted by blood transfusion. Specific genotypes of hepatitis B virus are increasingly recognized for their clinical significance and association with particular viral mutations. The aim of this study was to investigate the prevalence of hepatitis B virus genotypes in blood donors of Kerman, Yazd and Isfehan Blood Centers. Materials and Methods Sera of 120 blood donors, who were positive for hepatitis B surface antigen (HBsAg) using ELISA method, were selected. HBV DNA was extracted from sera using commercial kits and the p gene sequences were amplified by nested-PCR. HBV genotypes were determined by direct sequencing of the polymerase gene of HBV. The obtained sequences were aligned with Clustal W programme. Phylogenetic trees were constructed by the Neighbor-joining (NJ) method. The data were analyzed with Fisher test by SPSS 11.5 Results Out of 120 HBsAg positive volunteer blood donors, 69 (57.5% , CI 95% = 48.65-66.34)) were positive for HBV DNA sequence. Sequence analysis was done on the positive samples. Genotype D was detected among 100% of HBV infected volunteer blood donors. Conclusions The prevalence rate of genotype D was found to be 100%. These findings may have an impact on the immunological and genetic diagnosis of HBV as well as on the selection of diagnostic kits and viral quality control panels. Key words : Hepatitis B virus, Blood Donors, Genotype |
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Keywords: Hepatitis B virus, Blood Donors, Genotype |
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Full-Text [PDF 248 kb]
(3996 Downloads)
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Type of Study: Research |
Subject:
Infectious disease Published: 2014/08/17
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