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:: Volume 6, Issue 3 (Autumn 2009) ::
Sci J Iran Blood Transfus Organ 2009, 6(3): 181-189 Back to browse issues page
Study of in vitro properties of fibrin sealant prepared from single donor plasma
H. Abolghasemi , M. Amani , A. Jabari , R. Ranjbaran , M.H. Mohammadi , M. Habibi Roudkenar , A. Ali Balazadeh , A. Hashemi Teir , N. Amirizadeh , P. Eshghi
Abstract:   (16012 Views)

  Abstract

 Background and Objectives

 Fibrin sealant (FS) is a plasma derived product and has hemostatic, sealing and healing properties and is frequently used to reduce blood loss during and after surgery. Blood bank autologous fibrin sealants have no risk of transfusion transmitted diseases. The aim of this study was to obtain of thrombin and fibrinogen from FFP and study their in vitro properties for preparation of FS.

 Materials and Methods

 Fibrinogen was precipitated by use of protamin sulfate. Fibrinogen concentration was assayed with an enzyme-linked immunosorbent assay and clotting clauss method the effect of temperature on fibrinogen precipitation was also evaluated. Thrombin was prepared by manual method and TPD and its activity was then determined using specific chromogenic substrate spectrophotometric assay. Thrombin stability at different temperature degrees was evaluated and clotting time was measured. Tensile strength and adhesion strength were evaluated with the tensiometry device. Clot lysis time was determined by a clot solubility test in 5M urea.

 

 Results

 Fibrinogen concentration precipitated with protamin sulfate was measured as being 73 ± 8 mg/ml. The recovery of fibrinogen in cryoprecipitate was 93%. Thrombin mixed with fibrinogen had clot time of less than 5 seconds. Tensile strength and adhesion strength of fibrin sealant were 60 ± 8.9 g/cm2 and 55 ± 9 g, respectively. The average activity of the thrombin produced manually was 59.6 ± 6.2. Adding anti-fibrinolttic agent to fibrinogen concentrate has no effect on clotting time and tensile strength but it causes the stability improvement of fibrin clot.

 

 Conclusions

 Fibrinogen and thrombin prepared in this experiment have appropriate properties for production of fibrin sealants.

 

 Key words : Fibrin sealant, Fibrinogen, Thrombin, Plasma

 

Keywords: Fibrin sealant, Fibrinogen, Thrombin, Plasma
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Type of Study: Research | Subject: Hematology
Published: 2014/08/17
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Abolghasemi H, Amani M, Jabari A, Ranjbaran R, Mohammadi M, Habibi Roudkenar M, et al . Study of in vitro properties of fibrin sealant prepared from single donor plasma. Sci J Iran Blood Transfus Organ 2009; 6 (3) :181-189
URL: http://bloodjournal.ir/article-1-346-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 6, Issue 3 (Autumn 2009) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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