:: Volume 5, Issue 3 (Autumn 2008) ::
Sci J Iran Blood Transfus Organ 2008, 5(3): 157-166 Back to browse issues page
Molecular diagnosis of B cell Non-Hodgkin Lymphoma by evaluation of immunoglobulin heavy chain gene rearrangement
Abolfazl Yosefian, Behzad Poopak , Hassan Abolghasemi, Azita Azarkeivan, Mohammad Farhadilangerodi, Alireza Sadeghipour, Mojgan Jeyhonian, Mohammad Javadpourkhayat, Khandan Zare, Kobra Farahani, Mitra Salahmand
Abstract:   (24965 Views)
Molecular diagnosis of B cell Non-Hodgkin Lymphoma by evaluation of immunoglobulin heavy chain gene rearrangement Yousefian A.1(MS ), Poopak B.2(PhD), Abolghasemi H.1,3(MD), Azarkeivan A.1(MD), Farhadi Langroudi M.1(MD), Sadeghipour A.4(MD), Jeyhonian M.4(MD),Pourkhayat M.J.4(MD), Zare K.5(MD), Farahani K.2(BS), Salahmand M.6(BS) 1Iranian Blood Transfusion Organization-Research Center-Iran 2Islamic Azad University-Medical Branch. Tehran- Iran 3Baghiyatalah University of Medical Sciences-Molecular Biology Research Center, Tehran-Iran 4IranUniversity of Medical Sciences, Iran 5Shahid Beheshti University of Medical Sciences,Iran 6Payvand Medical Laboratory, Iran Abstract Background and Objectives Rearrangement of V, D, and J segments of immunoglobulin heavy chain gene with inserted or deleted nucleotides within rearranged segments makes unique hypervariable regions (CDR-3). These regions can be used for evaluation of B cell clonality for the purpose of molecular diagnosis of Non-Hodgkin Lymphoma (NHL) and for confirmatory diagnosis in suspicious cases. Materials and Methods In this study, samples of 42 patients were collected from Taleghani, Baqhiyatalah, and Aliasghar hospitals out of this number, there were 22 patients with diagnosis of B cell NHL, 10 with reactive hyperplasia, and 10 with malignant lymphoma. After DNA extraction from formalin fixed paraffin embedded tissues, PCR was done using consensus primers for amplification of CDR-3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis and silver stain. Results Clonal patterns in group 1 (B cell NHL), 2 (reactive and follicular hyperplasia), and 3 (morphological diagnosis without immunohistochemistry) were observed in 77.2%, 0%, and 70% of patients, respectively. Conclusions Our findings are compatible with other international studies with minor differences. The diagnosis of B-cell lymphoid malignancy can frequently be substantiated by detecting clonal immunoglobulin heavy chain (IGH ) gene rearrangement. Key words: Gene rearrangement, Non-Hodgkin Lymphoma, Immunoglobulin SJIBTO 2008 5(3): 157-166 Received: 31 Aug 2007 Accepted: 8 Oct 2008 Correspondence: Poopak B., PhD of Hematology. Islamic Azad University- Tehran Medical Branch. P.O.Box: 19295-1495 , Tehran, Iran, Tel: (+9821) 22006660 Fax : (+9821) 22264145 E-mail: bpoopak@yahoo.com
Keywords: Gene rearrangement, Non-Hodgkin Lymphoma, Immunoglobulin
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Type of Study: Research | Subject: Hematology
Published: 2014/08/17


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Volume 5, Issue 3 (Autumn 2008) Back to browse issues page