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Platelet antigens and antibodies in multitransfused patients
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E. Ebrahinzadeh   , M. Alaei  , Sh. Samiee , M. Shaiegan |
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| Keywords: Key words: Platelets, Antigens, Antibodies, PCR |
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Full-Text [PDF 605 kb]
(639 Downloads)
| Abstract (HTML) (1748 Views)
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Type of Study: Research |
Subject:
Blood transfusion medicine
Published: 2020/12/30
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Full-Text: (1467 Views) |
References:
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Sci J Iran Blood Transfus Organ 2020;17 (4): 294-307
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Platelet antigens and antibodies in multitransfused patients
Ebrahimzadeh E.1, Alaei M.1, Samiee Sh.1, Shaiegan M.1
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract
Background and Objectives
Following incompatible blood transfusions, anti-HLA and anti- HPA antibodies may develop and cause various disorders such as post-transfusion purpura, platelet refractoriness, and thrombocytopenia leading to bleeding. The aim of this study was to investigate platelet antigens and antibodies in multi-transfused patients.
Materials and Methods
In this descriptive study, platelet antigens and presence of platelet antibodies were tested by PCR-SSP and PIFT flowcytometry in 30 Thalassemia major and 30 oncohematologic patients with one hour post transfusion platelet counts in the range of 150000-450000/µl. χ2 test was employed for comparing the results of the study.
Results
Molecular genotyping of HPA-1in thalassemia major patients and patients with oncohematologic disorder in this study revealed that HPA-1a/1a was the most and HPA-1a/1b and HPA-3b/3b were the least frequent genotypes. No homozygous cases of 1b/1b, 2b/2b and 5b/5b were detected. HPA-4b allele was not detected in any patient. Flow PIFT results revealed platelet antibodies in 10(30%) patients with hematologic disorders and 1(3.3%) thalassemia major patients.
Conclusions
According to %100 frequency of HPA-4a and total absence of HPA-4b allele and absence of homozygous b/b genotype for HPA-1/-2/-5 alleles in the patient polulation of this study, the prevalence of platelet alloimmunization due to antibodies against these antigens seems to be low. Although further studies in the field are nessessary.
Key words: Platelets, Antigens, Antibodies, PCR
Received: 7 Oct 2020
Accepted:15 Dec 2020
Correspondence: Alaei M., MD. Pathologist. Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 82052234; Fax: (+9821) 88628741
E-mail: alaeimastaneh@hotmail.com |
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