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Effect of 8-week resistance training on HIF-1α gene expression and Endothelial Progenitor Cells recall of blood after one session of resistance activity in elderly men
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S. Karami    , F. Sdahidi , H. Rajabi , F. Golab |
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| Keywords: Key words: Resistance Training, Hypoxia-Inducible Factor 1, Endothelial Progenitor Cells |
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Full-Text [PDF 755 kb]
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| Abstract (HTML) (3076 Views)
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Type of Study: Research |
Subject:
Stem cells
Published: 2019/07/15
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Full-Text: (2261 Views) |
References:
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Sci J Iran Blood Transfus Organ 2019;16(2): 124-132
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Effect of 8-week resistance training on HIF-1α gene
expression and Endothelial Progenitor Cells recall of blood
after one session of resistance activity in elderly men
Karami S.1, Shahidi F.2, Rajabi H.3, Golab F.4
1Faculty of Physical Education and Sport Science, Shahid Rajaee Teacher Training University, Tehran, Iran
2Shahid Rajaee Teacher Training University, Tehran, Iran
3Kharazmi University, Tehran, Iran
4Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
Dysfunction of endothelial cells is associated with the development of atherosclerosis and cardiovascular disease. Adult endothelial progenitor cells are derived from hematopoietic stem cells capable of forming new blood vessels through a process of angiogenesis. This study was to investigate the effect of resistance training on HIF-1α gene expression and recall of Endothelial Progenitor Cells following an exercise session in the elderly.
Materials and Methods
In this quasi-experimental study, 24 elderly men were selected from among 100 elderly people and were randomly divided into two control and training groups. Blood samples were taken once before and after the protocol of one session of resistance training and once more before and after 8 weeks of resistance exercise. The flowcytometric and the RT-PCR methods were used for the enumaration and gene expression levels, respectively. The analysis of variance with repeated measurements of design was used.
Results
The values of HIF-1α expression in the training group were significant at the initial post-test (p = 0.048), secondary pre-test (p = 0.038) and post-test (p = 0.034). Also, the number of VEGFR2+ in the training group in the pre-test (p = 0.024) and secondary post-test (p = 0.002) and CD34+ in the pre-test (p = 0.019) and secondary post-test (p = 0.006) showed an increase.
Conclusions
Eight weeks of resistance training in the elderly can increase both the call of EPCs and expression of HIF-1α gene in the blood tissue leading to repair and maintenance of the layer of Endothelial cells and angiogenesis.
Key words: Resistance Training, Hypoxia-Inducible Factor 1, Endothelial Progenitor Cells
Received: 24 Feb 2019
Accepted: 8 May 2019
Correspondence: Karami S., Ph.D Student in Sport Physiology. Faculty of Physical Education and Sport Science, Shahid Rajaee Teacher Training University.
Postal Code: 1678815811, Tehran, Iran. Tel: (+9826) 34316349; Fax : (+9821) 34316349
E-mail: karami.sp@gmail.com
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Karami S, Sdahidi F, Rajabi H, Golab F. Effect of 8-week resistance training on HIF-1α gene expression and Endothelial Progenitor Cells recall of blood after one session of resistance activity in elderly men. Sci J Iran Blood Transfus Organ 2019; 16 (2) :124-132 URL: http://bloodjournal.ir/article-1-1249-en.html
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