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:: Volume 16, Issue 2 (Summer 2019) ::
Sci J Iran Blood Transfus Organ 2019, 16(2): 91-102 Back to browse issues page
Evaluation of platelet antigens and antibodies in patients with platelet refractoriness
M. Mostakhdemin Hosseini , Sh. Samiee , M. Shaiegan , S. Mohammadi , H. Jalaiekhoo , P. Tabatabiepanah , S. Modarresi
Keywords: Key words: Platelets, Flow Cytometry, Antibodies, Antigens
Full-Text [PDF 630 kb]   (1299 Downloads)     |   Abstract (HTML)  (3511 Views)
Type of Study: Research | Subject: Imunology
Published: 2019/07/15
Full-Text:   (4822 Views)
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Sci J Iran Blood Transfus Organ 2019; 16(2): 91-102
Original Article
 

 

Evaluation of platelet antigens and antibodies in patients
with platelet refractoriness
 
Mostakhdemin Hosseini M.1, Samiee Sh.1, Shaiegan M.1, Mohammadi S.2,
Jalaei-Kho H.3, Tabatabai Panah P.4, Modarresi S.1,5
 
1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
2Hematology, Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Shariati Hospital, Tehran, Iran
3School of Medicine, Aja University of Medical Sciences, Tehran, Iran
4Islamic Azad University, South Tehran Branch, Tehran, Iran
5Tehran Blood Transfusion Center, Tehran, Iran
 
 
 
 
Abstract
Background and Objectives
Immune platelet refractoriness is a complication of blood transfusion in patients with multiple platelet transfusions. Antibodies against surface antigens, including human platelet antigens (HPAs) and human leukocyte antigens (HLAs), can be produced which can cause the degradation of transfused platelets by macrophages. In this study, the frequency of platelet antigens and antibodies against both HPAs and HLAs is evaluated in patients with platelet refractoriness.
 
Materials and Methods
In this descriptive study, the simultaneous analyses of antigens of HPA-1/ -2/ -3/ -4/ -5/ -15 by molecular method (PCR-SSP), screening of platelet antibodies by flow cytometry, and anti-HLA-I antibodies by panel reactive antibody (PRA) assay were evaluated in 49 patients with platelet refractoriness.
 
Results
Out of 49 patients (including 18 females and 31 males) with platelet refractoriness, the platelet count in women was 6400 ± 3202 (range  of 1000-10000 cells/μl) and in men 7283 ± 2518 cell/μl (range of 3000-10000) showing no significance (p = 0.055). No case of HPA-4b, HPA-5b was found in this study. Anti-HLA antibodies and anti-HPA antibodies were detected in 61.2% and 4% of the patients, respectively.
 
Conclusions 
The data from our study showed that the frequencies of the allele a for HPA-1 / -3 / -4 / -5 and the allele b for HPA-2 / -15 were higher in the studied population. The frequencies of alleles and genotypes between the studied group and Iranian blood donors were different and can explain the occurrence of platelet resistance among the patients.
 
Key words: Platelets, Flow Cytometry, Antibodies, Antigens
 
Received: 23 Oct  2018
Accepted:12 May 2019
 

Correspondence: Shaiegan M., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 1157-14665 Tehran, Iran. Tel: (+9821) 88601599 ; Fax: (+9821) 88601599
E-mail:
mojganshaiegan@yahoo.com
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Mostakhdemin Hosseini M, Samiee S, Shaiegan M, Mohammadi S, Jalaiekhoo H, Tabatabiepanah P et al . Evaluation of platelet antigens and antibodies in patients with platelet refractoriness. Sci J Iran Blood Transfus Organ 2019; 16 (2) :91-102
URL: http://bloodjournal.ir/article-1-1233-en.html
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Volume 16, Issue 2 (Summer 2019) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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