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The allele frequencies of human Neutrophil Antigens 5 (HNA-5) in Tabriz city
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Atieh Khosravi   , Karim Shams Asanja , Mojgan Shaiegan , Shahram Samiei , Zahra Ataee , Maryam Abdollahi , Mahnaz Kavari , Mahboobeh Shafiei almasian , Parisa Hosseinzadeh |
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| Keywords: Key words: Neutrophils, PCR, Blood Donors |
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Type of Study: Research |
Subject:
Imunology
Published: 2017/06/17
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Full-Text: (1342 Views) |
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Sci J Iran Blood Transfus Organ 2016; 14(2): 84-91
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The allele frequencies of human Neutrophil Antigens
5 (HNA-5) in Tabriz city
Khosravi A.1, Shams Asanjan K.2,3, Shaiegan M.3, Samiee Sh.3, Ataee Z.3, Abdollahi M.3,
Kavari M.3, Shafiei Almasian M.3,4, Hosseinzadeh P.3,4
1Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
4Tabriz Educational Regional Blood Transfusion Center, Tabriz, Iran
Abstract
Background and Objectives
Human neutrophil antigens include 5 antigen systems. These antigens are on the plasma membrane of white blood cells. The aim of the study is to know about HNA allele frequency in the population in order to estimate the prevalence of the diseases related to HNA-5.
Materials and Methods
In this descriptive study, blood samples were taken from 190 Azari unrelated blood donors having referred to Tabriz Blood Center. DNA extraction was performed using commercial kit Pars Tous. HNA-5a and HNA-5b molecular were then evaluated by PCR-RFLP. PCR products were digested using enzymes Sdu1 Bsp1286I. HNA-5a + and HNA-5a - (or HNA-5b) antigen gene frequencies were calculated using the Hardy-Weinberg equation.
Results
HNA-5a (+/+), HNA-5a (+/-) and HNA-5a (-/-) phenotypes were observed in 93, 10 and 87 patients, respectively. The observed frequencies were consistent with Hardy-Weinberg equilibrium; allele frequencies of HNA-5a + and HNA-5a - (or HNA-5b) were equal to 0.51 and 0.49, respectively.
Conclusions
HNA-5 alleles frequency rates were in the same range reported for the other populations.
Key words: Neutrophils, PCR, Blood Donors
Received: 4 Feb 2017
Accepted: 24 Apr 2017
Correspondence: Shaiegan M., PhD of Immunology. Associate Professor of Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine.
P.O.Box: 14665-1157, Tehran, Iran. Tel: (+9821) 88601564; Fax: (+9821) 88601599
E-mail:
M.Shaiegan@ibto.ir
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khosravi A, Shams Asanja K, Shaiegan M, Samiei S, Ataee Z, Abdollahi M, et al . The allele frequencies of human Neutrophil Antigens 5 (HNA-5) in Tabriz city. Sci J Iran Blood Transfus Organ 2017; 14 (2) :84-91 URL: http://bloodjournal.ir/article-1-1106-en.html
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