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The effect of methanolic extract of aerial parts of Artemisiaannua on proliferation and apoptosis of acute lymphoblasticleukemia cell lines, Nalm-6 and Reh
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P. Mashati   , S. Esmaeili , N. Dehghan Nayeri , M. Darvishi , A. Gharehbaghian |
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| Keywords: Key words: Acute Lymphoid Leukemia, Artemisia annua, Apoptosis |
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Type of Study: Research |
Subject:
Hematology
Published: 2017/03/13
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Full-Text: (2470 Views) |
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Sci J Iran Blood Transfus Organ 2017; 13(4): 304-313
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The effect of extract from aerial parts of Juniperus excelsa
plant on proliferation and apoptosis of acute lymphoblastic leukemia cell lines, Nalm-6 and Reh
Darvishi M.1, Esmaeili S.2, Dehghan-Nayeri N.1, Mashati P.1, Gharehbaghian A.1,3
1School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Traditional Medicine and Materia Medica Research Center, School of traditional medicines, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
3Pediatric congenital hematologic disorders research center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Background and Objectives
Acute lymphoblastic leukemia (ALL) is the most common childhood malignancy. The side effects of chemotherapeutic drugs have primarily lead to the increased use of natural products for cancer treatment. Juniperus excelsa, a medicinal herb, was reported to show antiproliferative effects on various cancer cells. In this study, cytotoxic and apoptotic effects of extract from aerial parts of Juniperus excelsa plant were investigated on acute lymphoblastic leukemia cell lines, Nalm-6 and Reh.
Materials and Methods
In this basic research, Nalm-6 and Reh were cultured and then they were treated with various concentrations of J. excelsa extract for 48 and 72 h and then the cell viability was evaluated by using MTT assay. Apoptosis also was assessed by caspase 3 activity assay and flow cytometry following Annexin V and Propidium iodide staining. Statistical analysis was assessed by one-way ANOVA test and SPSS 23.
Results
MTT assay results show that J. excelsa extract concentrations of 3, 4 and 5 µg/ml significantly reduce percentage of alive cells (p < 0.001). Flow cytometry results also show that J. excelsa extract significantly increase percentage of apoptotic cells compared with control groups (p < 0.001). Caspase 3 activity assay results show that caspase 3 activity was significantly increased in treated cells (p < 0.05).
Conclusions
Our study shows that extract from aerial parts of J. excelsa has cytotoxic and apoptotic effects on Nalm-6 and Reh cells.
Key words: Acute lymphoblastic leukemia, Juniperus excelsa, cytotoxicity, Apoptosis
Received: 5 Oct 2016
Accepted: 9 Nov 2016
Correspondence: Gharehbaghian A., PhD in Clinical Immunohematology. School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences and Pediatric congenital hematologic disorders research center, Shahid Beheshti University of Medical Sciences.
P.O.Box: 15468-15514, Tehran, Iran. Tel: (+9821) 22721150; Fax: (+9821) 22731999
E-mail: gharehbaghian@sbmu.ac.ir
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Mashati P, Esmaeili S, Dehghan Nayeri N, Darvishi M, Gharehbaghian A. The effect of methanolic extract of aerial parts of Artemisiaannua on proliferation and apoptosis of acute lymphoblasticleukemia cell lines, Nalm-6 and Reh. Sci J Iran Blood Transfus Organ 2017; 14 (1) :34-42 URL: http://bloodjournal.ir/article-1-1085-en.html
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