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:: Volume 14, Issue 1 (Spring 2017) ::
Sci J Iran Blood Transfus Organ 2017, 14(1): 34-42 Back to browse issues page
The effect of methanolic extract of aerial parts of Artemisiaannua on proliferation and apoptosis of acute lymphoblasticleukemia cell lines, Nalm-6 and Reh
P. Mashati , S. Esmaeili , N. Dehghan Nayeri , M. Darvishi , A. Gharehbaghian
Abstract:   (5425 Views)

Abstract

Background and Objectives

Acute lymphoblastic leukemia (ALL) is the most common malignancy among children. The use of natural compounds has invoked a lot of interest in treatment of malignancies due to the adverse effects of chemotherapy treatment. Artemisia annua was reported to show cytotoxic effects on various cancer cell lines. In this study, cytotoxic effects of Artemisia annua extract were investigated on acute lymphoblastic leukemia cell lines.  

Materials and Methods

In this basic research, Nalm-6 and Reh cells were cultured and then treated with various concentrations of Artemisia annua extract; afterwards, the cell viability was evaluated using MTT assay for 48 and 72 h. The Caspase 3 activity assay and flow cytometry following Annexin V and Propidium iodide staining were used to assess apoptosis. Statistical analysis was evaluated by one-way ANOVA test.

Results

A. annua extract showed IC50 of 90 µg/ml on Nalm-6 and IC50 of 70 µg/ml on Reh cells after 48 h; the cytotoxic effect after 72 h was noticeable (p < 0.001). Flow cytometry results also show that A. annua extract concentration of 40 µg/ml increases the percentage of apoptotic cells compared with control groups (p < 0.05). Significant increase in Caspase 3 activity was observed after treatment with Artemisia annua compared with control groups (p < 0.01).

Conclusions 

Our results show that methanolic extract of aerial parts of A. annua exerting cytotoxic and inhibitory effects on Nalm-6 and Reh cells.

Key words: Acute Lymphoid Leukemia, Artemisia annua, Apoptosis

Keywords: Key words: Acute Lymphoid Leukemia, Artemisia annua, Apoptosis
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Type of Study: Research | Subject: Hematology
Published: 2017/03/13
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Mashati P, Esmaeili S, Dehghan Nayeri N, Darvishi M, Gharehbaghian A. The effect of methanolic extract of aerial parts of Artemisiaannua on proliferation and apoptosis of acute lymphoblasticleukemia cell lines, Nalm-6 and Reh. Sci J Iran Blood Transfus Organ 2017; 14 (1) :34-42
URL: http://bloodjournal.ir/article-1-1085-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 14, Issue 1 (Spring 2017) Back to browse issues page
فصلنامه پژوهشی خون Scientific Journal of Iran Blood Transfus Organ
The Scientific Journal of Iranian Blood Transfusion Organization - Copyright 2006 by IBTO
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